Major lipid classes and their fatty acids in a parasitic nematode, Ascaridia galli

Abstract

The paper presents major lipid classes and their fatty acids investigated from Ascaridia galli, a nematode parasite of country fowl. Thin layer chromatography (TLC) reveals that the percent of total lipid, neutral lipid, phospholipids, and glycolipids are 1.94, 54.39, 26.95 and 18.66, respectively. Gas-liquid chromatography (GLC) analysis shows that the saturated fatty acids are the major components in all the lipid fractions followed by monoenes and dienes. Polyunsaturated fatty acids (PUFA) were present in low amount. Stearic acids (C 18 ) were the chief components among all the fatty acids in all the lipid fractions.

Variation in lipid and fatty acid uptake among nematode and cestode parasites and their host, domestic fowl: host–parasite interaction

“…NL content of A. galli is very high (64.26 %), whereas in R. (F.) echinobothrida, it is 53.23 %, but liver shows less amount (38.87 %). In an earlier study the percentage of NL in R. (F.) echinobothrida was 64.39 (Mondal et al 2009), whereas, in A. galli it was recorded as 54.39 (Ghosh et al 2010). Variation in NL content in parasites may be related with the feeding habit of the host.…”

“…Ascaris lumbricoides (8.9 %) and Nippostrongylus brasiliensis (11.9 %). However, 1.94 % of TL was found in A. galli (Ghosh et al 2010). These variations might occur due to the availability of food materials, which is influenced by the food habit of the host, in the different microhabitat of the parasites.…”

“…Jadhav (2008) commented that mostly all the parasites absorb most of the food materials from host and fulfilling its need for development. A preliminary study has been made on major lipid classes and their fatty acids of Raillietina (Fuhrmannetta) echinobothrida (Mondal et al 2009) and of Ascaridia galli (Ghosh et al 2010). The total analysis of major lipid classes and fatty acids composition has already been done on trematode parasite, Paramphistomum cervi, rumen fluid and liver of the mammalian host, Capra hircus (Ghosh and Misra 2011).…”

Lipid profiling of the filarial nematodes Onchocerca volvulus, Onchocerca ochengi and Litomosoides sigmodontis reveals the accumulation of nematode-specific ether phospholipids in the host

“…Methods such as TLC cannot provide information about molecular species composition, which could serve as putative biomarkers. In other reports, TLC and gas chromatography (GC) were combined to analyze the lipid classes and fatty acids of Brugia malayi ( Smith et al, 1996 , Longworth et al, 1987 ) and of the avian parasite Ascaridia galli ( Ghosh et al, 2010 ). However, no targeted analysis of phospholipid molecular species of O. volvulus and related nematodes by state-of-the-art LC-MS technology has, to our knowledge, been conducted to date.…”

Metabolite Variation between Nematode and Bacterial Seed Galls in Comparison to Healthy Seeds of Ryegrass Using Direct Immersion Solid-Phase Microextraction (DI-SPME) Coupled with GC-MS

“…In our study, the presence of the derivatives of methyl, ethyl and vinyl esters in the nematode galls could indicate the presence of pheromones associated with A. funesta . Nematodes are also known to contain significant quantities of lipids in their body cuticle [ 42 ], and fatty acids and fatty aldehydes are major components of the nematode cuticle [ 43 ]. Phospholipids play an important role in the formation of nematode cellular membranes and in signal transduction pathways [ 44 ].…”