Major Outer Membrane Proteins from Many
            <i>Campylobacter</i>
            Species Cross-React with Cholera Toxin

Albert, M. John; Haridas, Shilpa; Adler, Ben

doi:10.1128/cvi.00471-07

Cited by 4 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This possibility should be explored by immunization with CT-B and cholera vaccines. As all clinically important species of Campylobacter react with CT antibody (32), it is possible to control all Campylobacter species diarrheas with cholera vaccines. There will be significant economic benefits if we can use the existing cholera vaccines, as this will obviate the need for developing new vaccines against Campylobacter spp.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

Albert

Mustafa

Islam

et al. 2013

mBio

Self Cite

View full text Add to dashboard Cite

Immunity to Campylobacter jejuni, a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adler, Infect. Immun. 75:3070–3073, 2007), we demonstrated that antibody to cholera toxin (CT) cross-reacted with the major outer membrane proteins (MOMPs) of all Campylobacter jejuni strains tested. In the current study, we investigated whether immunization with CT protects against intestinal colonization by C. jejuni in an adult mouse model and whether the nontoxic subunit of CT (CT-B) is the portion mediating cross-reaction. Mice were orally immunized with CT and later challenged with C. jejuni strains (48, 75, and 111) of different serotypes. Control animals were immunized with phosphate-buffered saline. Fecal shedding of challenge organisms was studied daily for 9 days. Serum and fecal antibody responses were studied by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The cross-reactivity of rabbit CT-B antibody to MOMP was studied by immunoblotting. The reactivity of 21 overlapping 30-mer oligopeptides (based on MOMP’s sequence) against rabbit CT antibody was tested by ELISA. Test animals produced antibodies to CT and MMP in serum and feces and showed resistance to colonization, the vaccine efficacies being 49% (for strain 48), 37% (for strain 75), and 34% (for strain 111) (P, ≤0.05 to ≤0.001). One peptide corresponding to a variable region of MOMP showed significant reactivity. CT-B antibody cross-reacted with MOMP. Since CT-B is a component of oral cholera vaccines, it might be possible to control C. jejuni diarrhea with these vaccines.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Overlapping 30-mer peptides (Thermo, Fisher Scientific, Ulm, Germany) based on the complete MOMP (PorA) sequence of C. jejuni NCTC 11168 (36) were used for epitope mapping. The 21 peptides (Table 3) were dissolved in PBS (pH 7.2) to a concentration of 1 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

Albert

Mustafa

Islam

et al. 2013

mBio

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have shown that rabbit polyclonal antibodies to cholera toxin [CT] react with the major outer membrane protein (MOMP) of C . jejuni [ 9 ] and several other species of the Campylobacter genus [ 10 ]. Furthermore, oral immunization of adult mice with CT afforded significant protection against intestinal colonization with C .…”

Section: Introductionmentioning

confidence: 99%

Immunization with a Double-Mutant (R192G/L211A) of the Heat-Labile Enterotoxin of Escherichia coli Offers Partial Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

et al. 2015

Self Cite

View full text Add to dashboard Cite

We have previously shown that antibodies to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from Campylobacter jejuni strains on Western blot. Further, oral immunization with CT significantly protected against challenge with C. jejuni in an adult mouse colonization model of infection. CT and the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli are structurally and functionally related. LT and its mutants including the double-mutant LT (R192G/L211A) (dmLT), are powerful mucosal adjuvants. Unlike LT which is reactogenic, dmLT has been shown to be safe for human use. In the current study, we determined whether rabbit anti-dmLT antibodies reacted with MOMPs from C. jejuni strains and whether immunization with dmLT would afford protection against C. jejuni. On Western blot, the MOMPs from C. jejuni 48 (Penner serotype O:19), C. jejuni 75 (O:3) and C. jejuni 111 (O:1,44) were probed with rabbit antibodies to dmLT or LT-E112K (a non-toxic LT mutant), which showed a lack of reaction. Adult BALB/c mice were orally immunized with dmLT and orally challenged with C. jejuni 48 or 111. Protection from colonization with the challenge bacteria was studied by enumerating Campylobacter colonies in feces daily for 9 days. Vaccination produced robust serum and stool antibody responses to dmLT and no antibody responses to C. jejuni MOMP. Vaccinated mice showed reduced colonization and excretion of both challenge strains compared to control mice. However, the differences were not statistically significant. The protective efficacy of the dmLT vaccine varied from 9.1% to 54.5%. The lack of cross-reaction between the MOMP and dmLT suggests that protection is not mediated by cross-reacting antibodies, but may be due to activation of innate immunity. As dmLT is safe for humans, it could be incorporated into a C. jejuni vaccine to enhance its efficacy.

show abstract

“…Molecular confirmation of species was carried out by PCR as described previously (1). The molecular fingerprint of each C. jejuni strain was identified by flaA-restriction fragment length polymorphism (RFLP) described elsewhere (2).…”

mentioning

confidence: 99%

Recombinant PorA, the Major Outer Membrane Protein ofCampylobacter jejuni, Provides Heterologous Protection in an Adult Mouse Intestinal Colonization Model

Islam

Albert

2010

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

Immunity against Campylobacter jejuni, a major food-borne pathogen causing diarrhea, is largely serotype specific. The major outer membrane protein (MOMP) of C. jejuni, PorA, is a common antigen with the potential to provide broad protection. Adult BALB/c mice were orally immunized with a recombinant glutathione S-transferase (GST) fused to PorA prepared from Campylobacter jejuni C31 (O:6,7) (GST-PorA) combined with a modified heat-labile enterotoxin of Escherichia coli as an adjuvant and later orally challenged with C31 strain or three heterologous strains: 48 (O:19), 75 (O:3), and 111 (O:1,44). Protection from colonization with the challenge organism was studied by fecal screening daily for 9 days. Serum and intestinal lavage fluid antibodies against the vaccine and Sarkosyl-purified MOMP from C31 were measured by using an enzyme-linked immunosorbent assay. The vaccine produced robust antibody responses against both antigens in serum and secretion. Since strain C31 was a poor colonizer, homologous protection could not be studied. The protective efficacies of heterologous strains were 43% (for strain 48, P < 0.001), 29% (for strain 75, P < 0.005), and 42% (for strain 111, P < 0.001) for the 9-day period compared to control mice given phosphate-buffered saline. Thus, PorA provided appreciable protection against colonization with heterologous serotypes.Campylobacter jejuni is a food-borne pathogen and a leading cause of diarrhea worldwide (19,33,34). Campylobacteriosis is associated with a number of important sequelae, including Guillain-Barre syndrome, Reiter's syndrome, reactive arthritis, and irritable bowel syndrome (10, 37). C. jejuni also contributes to significant mortality of children in developing countries (18). In view of significant morbidity, mortality, and economic burden associated with C. jejuni infection, the control and prevention of campylobacteriosis are a priority. One possible tool for the prevention of campylobacteriosis is vaccination. It has been observed that C. jejuni infection results in the acquisition of immunity. However, immunity in humans seems to be serotype (Penner serotype) specific (13, 36). There are numerous serotypes of C. jejuni according to the Penner serotyping scheme, which is based on the lipopolysaccharide capsule (25). Therefore, vaccines based on common antigens that are shared by all serotypes seem attractive for broad protection. One such antigen is the major outer membrane protein (MOMP) of C. jejuni, PorA, which is present in abundant quantity in the organism. There are three conformational forms of MOMP: folded monomer (35 kDa), denatured monomer (45 kDa), and the native trimer (120 to 140 kDa). Only the folded monomer and the native trimer have pore-forming activities (44). The MOMP is involved in ion transport across the bacterial cell wall and adhesion of the bacterium to the intestinal mucosa (30, 41). It consists of 18 ␤-strands connected by short periplasmic turns and nine external loops. The external loops are antigenically variable (44). This is evident...

show abstract

Major Outer Membrane Proteins from Many Campylobacter Species Cross-React with Cholera Toxin

Cited by 4 publications

References 38 publications

Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

Immunization with a Double-Mutant (R192G/L211A) of the Heat-Labile Enterotoxin of Escherichia coli Offers Partial Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

Recombinant PorA, the Major Outer Membrane Protein ofCampylobacter jejuni, Provides Heterologous Protection in an Adult Mouse Intestinal Colonization Model

Contact Info

Product

Resources

About