Major Sensing Proteins in Pathogenic Fungi: The Hybrid Histidine Kinase Family

Hérivaux, Anaïs; So, Yee Seul; Gastebois, Amandine; Latgé, Jean Paul; Bouchara, Jean Philippe; Bahn, Yong-Sun; Papon, Nicolas

doi:10.1371/journal.ppat.1005683

Cited by 52 publications

(49 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histidine kinases are known to transmit information about environmental stimuli through signal-transduction pathways, and the OS-1 R572H mutation is in the sensor domain of this protein, specifically in the semistructured connector between the two α-helices of the HAMP domain (present in histidine kinases, adenyl cyclases, methyl-accepting proteins, and phosphatases) (19,20).…”

Section: R572hmentioning

confidence: 99%

Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Huberman

Coradetti

Glass

2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Identifying nutrients available in the environment and utilizing them in the most efficient manner is a challenge common to all organisms. The model filamentous fungus Neurospora crassa is capable of utilizing a variety of carbohydrates, from simple sugars to the complex carbohydrates found in plant cell walls. The zinc binuclear cluster transcription factor CLR-1 is necessary for utilization of cellulose, a major, recalcitrant component of the plant cell wall; however, expression of clr-1 in the absence of an inducer is not sufficient to induce cellulase gene expression. We performed a screen for unidentified actors in the cellulose-response pathway and identified a gene encoding a hypothetical protein (clr-3) that is required for repression of CLR-1 activity in the absence of an inducer. Using clr-3 mutants, we implicated the hyperosmotic-response pathway in the tunable regulation of glycosyl hydrolase production in response to changes in osmolarity. The role of the hyperosmotic-response pathway in nutrient sensing may indicate that cells use osmolarity as a proxy for the presence of free sugar in their environment. These signaling pathways form a nutrient-sensing network that allows N. crassa cells to tightly regulate gene expression in response to environmental conditions. MAP kinase cascade | carbon metabolism | hyperosmotic response | carbon catabolite repression | plant biomass degradation

show abstract

Section: R572hmentioning

confidence: 99%

Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Huberman

Coradetti

Glass

2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Protein names are those of S. cerevisiae , if different those of N. crassa are indicated in brackets. Full arrows indicate positive regulations, hashed arrows indicate either positive or negative regulations (different among fungal species), or direct interactions that remain to be demonstrated (reviewed in Bahn, 2008; Tanaka and Izumitsu, 2010; Jung et al, 2012; Herivaux et al, 2016). (B) Protein structure of class III fungal HHKs.…”

Section: From Phenylpyrroles To Osmotic Signal Transductionmentioning

confidence: 99%

“…Fungal HHKs are composed of the variable N-terminal sensor domain and the C-terminal domain, including the catalytic HK and ATPase domains that autophosphorylate the conserved histidine residue, in addition to the receiver domain with the cognate aspartate residue (reviewed in Jung et al, 2012; Herivaux et al, 2016) ( Figure 2 ). A classification according to the structural components of the N-terminal domain and the peptide sequence around the conserved histidine residue attributed 16 classes of HHKs to fungi (Defosse et al, 2015).…”

Section: Fungal Histidine Kinases Linked To Phenylpyrrole Resistancementioning

confidence: 99%

“…Altogether these results are in agreement with the hypothesis that in most cases loss-of-function mutations are responsible for fludioxonil resistance in plant pathogenic fungi (mainly laboratory mutants; reviewed in Defosse et al, 2015), but mutations leading to modified function or even constitutively active HHK may exist as well, probably at very low frequencies. Due to its essential role in many biological processes including pathogenicity (Viaud et al, 2006; Herivaux et al, 2016), loosing a class III HHK might explain the absence of fludioxonil field resistance in most plant pathogenic fungi.…”

Section: Fungal Histidine Kinases Linked To Phenylpyrrole Resistancementioning

confidence: 99%

See 1 more Smart Citation

Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance

Kilani¹,

Fillinger²

2016

Front. Microbiol.

106

View full text Add to dashboard Cite

Phenylpyrroles are chemical analogs of the natural antifungal compound pyrrolnitrin. Fenpiclonil, but mainly fludioxonil are registered against multiple fungal crop diseases since over 25 years for seed or foliar treatment. They have severe physiological impacts on the pathogen, including membrane hyperpolarization, changes in carbon metabolism and the accumulation of metabolites leading to hyphal swelling and burst. The selection and characterization of mutants resistant to phenylpyrroles have revealed that these fungicides activate the fungal osmotic signal transduction pathway through their perception by a typical fungal hybrid histidine kinase (HHK). The HHK is prone to point mutations that confer fungicide resistance and affect its sensor domain, composed of tandem repeats of HAMP motifs. Fludioxonil resistant mutants have been selected in many fungal species under laboratory conditions. Generally they present severe impacts on fitness parameters. Since only few cases of field resistance specific to phenylpyrroles have been reported one may suspect that the fitness penalty of phenylpyrrole resistance is the reason for the lack of field resistance.

show abstract

“…Two-component signal transduction pathways play important roles in the biology of prokaryotic and eukaryotic microorganisms (Wuichet et al, 2010;Capra and Laub, 2012;H erivaux et al, 2016). Response-regulator systems occur in most fungi (Schaller et al, 2011), and compelling evidence indicates that they originate from multiple independent horizontal gene transfer events from bacteria to eukaryotes, that occurred after the divergence of Archaea, Eubacteria and Eukarya (Wuichet et al, 2010, Schaller et al, 2011.…”

Section: Conserved and Divergent Functions Of Skn7mentioning

confidence: 99%

The two‐component response regulator Skn7 belongs to a network of transcription factors regulating morphogenesis in Candida albicans and independently limits morphogenesis‐induced ROS accumulation

Basso

Znaidi

Lagage

et al. 2017

Molecular Microbiology

View full text Add to dashboard Cite

Skn7 is a conserved fungal heat shock factor-type transcriptional regulator. It participates in maintaining cell wall integrity and regulates the osmotic/oxidative stress response (OSR) in S. cerevisiae, where it is part of a two-component signal transduction system. Here, we comprehensively address the function of Skn7 in the human fungal pathogen Candida albicans. We provide evidence reinforcing functional divergence, with loss of the cell wall/osmotic stress-protective roles and acquisition of the ability to regulate morphogenesis on solid medium. Mapping of the Skn7 transcriptional circuitry, through combination of genome-wide expression and location technologies, pointed to a dual regulatory role encompassing OSR and filamentous growth. Genetic interaction analyses revealed close functional interactions between Skn7 and master regulators of morphogenesis, including Efg1, Cph1 and Ume6. Intracellular biochemical assays revealed that Skn7 is crucial for limiting the accumulation of reactive oxygen species (ROS) in filament-inducing conditions on solid medium. Interestingly, functional domain mapping using site-directed mutagenesis allowed decoupling of Skn7 function in morphogenesis from protection against intracellular ROS. Our work identifies Skn7 as an integral part of the transcriptional circuitry controlling C. albicans filamentous growth and illuminates how C. albicans relies on an evolutionarily-conserved regulator to protect itself from intracellular ROS during morphological development.

show abstract

Major Sensing Proteins in Pathogenic Fungi: The Hybrid Histidine Kinase Family

Cited by 52 publications

References 32 publications

Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance

The two‐component response regulator Skn7 belongs to a network of transcription factors regulating morphogenesis in Candida albicans and independently limits morphogenesis‐induced ROS accumulation

Contact Info

Product

Resources

About