Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species

Fouts, Derrick E.; Mongodin, Emmanuel F.; Mandrell, Robert E.; Miller, William G.; Rasko, David A.; Ravel, Jacques; Brinkac, Lauren; DeBoy, Robert T.; Parker, Craig T.; Daugherty, Sean C.; Dodson, Robert J.; Durkin, A. Scott; Madupu, Ramana; Sullivan, Steven A.; Shetty, Jyoti; Ayodeji, Mobolanle; Shvartsbeyn, Alla; Schatz, Michael C.; Badger, Jonathan H.; Fraser, Claire M.; Nelson, Karen E.

doi:10.1371/journal.pbio.0030015

Cited by 485 publications

(448 citation statements)

References 99 publications

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“…Of course, future biochemical and genetic studies are needed to fully define the function of C. rectus 314 ciaB. CiaB genes have been identified from several species of campylobacters that do not typically reside in the oral cavity of humans including C. jejuni, C. lari, C. coli, C. fetus, and C. upsaliensis [21,22]. However, during the course of characterizing the ciaB from C. rectus 314, potential ciaB genes were identified among campylobacters that do reside within the oral cavity of humans (Table 3) including C. rectus 33238 (ZP03610758.1, annotated at NCBI as a lipoprotein signal peptidase), C. curvus (YP001407631.1, annotated as a hypothetical protein), and C. concisus (EAT98433.1, 4 Agarose gel of C. rectus DNA amplified using primers designed to amplify the complete ciaB gene ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…C. curvus, like C. rectus, is an oral bacteria associated with periodontitis [36]. BLASTx analysis of the amplicon from strain 314 revealed a gene fragment sharing sequence identity and similarity (53 and 70 %, respectively) to the [21] ciaB gene from C. jejuni (NCBI, AAD38497.1). The sequence of the 448 base pair amplicon was used as a template for PCR-based gene walking.…”

Section: Identification Of C Rectus 314 Ciabmentioning

confidence: 99%

“…Among the best studied C. jejuni virulence factors is the ciaB gene, which has been shown to play a critical role in host cell invasion; and therefore pathogenesis [18][19][20]. In addition, ciaB genes have been identified from additional campylobacters including Campylobacter lari, Campylobacter coli, and Campylobacter upsaliensis [21,22]. Given the conservation of ciaB, and a recent study showing C. rectus 314 can invade host cells [23], we hypothesized that the C. rectus 314 contains a ciaB gene.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

LaGier

Threadgill

2013

Indian J Microbiol

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The oral bacterium, Campylobacter rectus, is an etiological agent of periodontitis. The virulence genes of C. rectus are largely unknown. The aim of this study was to query C. rectus for the presence of an invasion antigen B (ciaB) gene, which is needed for cell invasion by the related species Campylobacter jejuni. PCR and PCRwalking identified a ciaB from C. rectus. In silico analyses of C. rectus 314 ciaB (Cr-ciaB) revealed an ORF of 1,830 base pairs. The Cr-CiaB protein shared significant sequence identity (BLASTx and phylogeny) with CiaB from related campylobacters. Cr-CiaB is predicted to lack membrane helices, signal peptides, and localizes to the cytoplasm; which are consistent with CiaB proteins. Expression of Cr-ciaB was confirmed with RT-PCR; and potential ciaB genes were detected in eight additional strains of C. rectus. Cr-ciaB is the first CiaB identified from the oral campylobacters.

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Section: Discussionmentioning

confidence: 99%

Section: Identification Of C Rectus 314 Ciabmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

LaGier

Threadgill

2013

Indian J Microbiol

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“…IVSs are absent from all three 23S rRNA gene copies of C. jejuni NCTC 11168 (32) but were detected in other strains of C. jejuni and C. coli (29,33,41) and were also identified in all three copies of the 23S rRNA gene in the recently sequenced genome of C. coli RM2228 (12). When present, the IVS is located only 891 nucleotides (nt) upstream from the site in the 23S rRNA gene where a point mutation (A2075G) has been associated with high-level resistance to erythromycin in Campylobacter (12,14,20). Surveys of C. jejuni from broilers showed that only a minority of strains (approximately 10%) contained IVSs (29,33).…”

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confidence: 99%

The Absence of Intervening Sequences in 23S rRNA Genes of Campylobacter coli Isolates from Turkeys Is a Unique Attribute of a Cluster of Related Strains Which Also Lack Resistance to Erythromycin

Chan

Miller

Mandrell

et al. 2007

Appl Environ Microbiol

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Certain Campylobacter strains harbor a transcribed intervening sequence (IVS) in their 23S rRNA genes. Following transcription, the IVS is excised, leading to fragmentation of the 23S rRNA. The origin and possible functions of the IVS are unknown. Furthermore, the distribution of IVS-harboring strains within Campylobacter populations is poorly understood. In this study, 104 strains of Campylobacter coli from turkeys, representing 27 different multilocus sequence typing-based sequence types (STs), were characterized in terms of IVS content and erythromycin susceptibility. Sixty-nine strains harbored IVSs in all three 23S rRNA genes, whereas the other 35 strains lacked IVSs from at least one of the genes. The STs of the latter strains belonged to an unusual cluster of C. coli STs (cluster II), earlier found primarily in turkey strains and characterized by the presence of the C. jejuni aspA103 allele. The majority (66/69) of strains harboring IVSs in all three 23S rRNA genes were resistant to erythromycin, whereas none of the 35 strains with at least one IVS-free 23S rRNA gene were resistant. Cluster II strains could be transformed to erythromycin resistance with genomic DNA from C. coli that harbored IVS and the A2075G transition in the 23S rRNA gene, associated with resistance to erythromycin in Campylobacter. Erythromycin-resistant transformants harbored both the A2075 transition and IVS. The findings suggest that the absence of IVS in C. coli from turkeys is characteristic of a unique clonal group of erythromycin-susceptible strains and that IVS can be acquired by these strains via natural transformation to erythromycin resistance.

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“…A ligação desta toxina à superfície da célula hospedeira é mediada pelas proteínas CdtA e CdtC seguida da entrega de CdtB ao núcleo celular do hospedeiro (Pickett et al, 1996;Fouts et al, 2005;Asakura et al, 2008). Dessa maneira, a bactéria causa danos ao DNA da célula hospedeira e promove a interrupção do ciclo celular.…”

Section: Manifestações Clínicas E Patogêneseunclassified

Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Gomes¹

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Este espaço é dedicado àqueles que, de alguma forma, contribuíram para que esta dissertação fosse realizada. Não sendo viável nomeá-los a todos, há no entanto alguns a quem não posso deixar de manifestar o meu apreço e agradecimento sincero.Á Deus pela benção da vida, proteção e força para superar os obstáculos ao longo da minha jornada.À Profª Drª Juliana Pfrimer Falcão, pela orientação, pelo carinhoso apoio, partilha do saber e as valiosas contribuições para a realização desse trabalho. Acima de tudo, obrigada por continuar a acompanhar-me nesta jornada e por estimular o meu interesse pelo conhecimento. Agradeço pela confiança em mim depositada, pelo empenho, paciência e o exemplo profissional e moral durante todo esse período. Minha profunda admiração. Muito Obrigada por tudo! Á minha querida e amada Mãe pelo amor sem reservas, pelos momentos de carinho e por tornar a minha vida mais colorida. Ao meu Pai pela figura firme e segura que me empurra nos momentos mais difíceis, por tornar possível os meus sonhos e conquistas. Não tenho palavras para descrever esse Amor! Aos meus queridos irmãos Gabriela, Marcos, Carina e ao meu sobrinho João Vitor pelo incentivo, apoio e carinho. Vocês são parte de mim! Á aquele que não leva jeito com as palavras, mas tem um abraço que me acalma, um olhar que me alegra, um sorriso que conforta e um amor que me transforma. Alessandro, "Não me lembro mais qual foi nosso começo. Sei que não começamos pelo começo. Já era amor antes de ser"! strains; the flhA gene was detected in 11 (17.5%) strains; the dnaJ gene was detected in 10 (15.9%) strains; the pldA gene was detected in 7 (11.1%) strains ; the iamA gene was detected in three (4.8%) strains; the cdtC and docA genes were found in two (3.2%) strains; the cdtA and crsA were found in one (1.6%) strain and the ciaB, wlaN, virB11 and racR genes were not detected. Among the 63 strains studied, 42 were susceptible to all antimicrobials tested. Of the 21 resistant strains, 10 (15.9%) were resistante to tetracycline and doxaciclyne, six (9.5%) showed resistance to ciprofloxacin, and one (1.6%) was resistant to erythromycin. Only four (6.3%) strains were simultaneously resistant to at least two different classes of the antibiotics tested. The dendrogram of genetic similarity of Pulsed field gel electrophoresis (PFGE) grouped the 63 strains studied into two groups namely PFGE-A and PFGE-B with a genomic similarity of 44.9% among them. However, some strains isolated from humans, animals, the environment and food presented a high genotypic similarity above 80% and were subdivided into seven groups designated as PFGE-A1 to PFGE-A7. The dendrogram of genetic similarity of the SRV-flaA gene sequences grouped the strains studied into two groups namely SVR-A and SVR-B, with similarity above 83.1% among them. Besides, the deposit of the SVR sequences of the flaA gene in the online database showed that the alleles 30 and 1647 were the iv most frequently found and allowed the comparison between the strains studied with the alleles described in the d...

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Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species

Cited by 485 publications

References 99 publications

Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

The Absence of Intervening Sequences in 23S rRNA Genes of Campylobacter coli Isolates from Turkeys Is a Unique Attribute of a Cluster of Related Strains Which Also Lack Resistance to Erythromycin

Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

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