‘MAK-10’: A Long Shelf-life Charentais Breeding Line Developed by Introgression of a Genomic Region from Makuwa Melon

Perpiñá, Gorka; Cebolla-Cornejo, Jaime; Esteras, Cristina; Monforte, Antonio J.; Picó, Belén

doi:10.21273/hortsci12068-17

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…The ethylene pathway has also been studied in melon [ 8 – 12 ], which is considered a distinctive model for the study of fruit ripening behavior, as the full spectrum of non-climacteric to climacteric behavior is represented across its natural variation [ 13 ]. As a result, genotypes may display different combinations of these behaviors as recently documented–aromatic individuals that do not abscise or do not change external color and flesh softening that happens in both climacteric and non-climacteric backgrounds [ 14 , 15 ]. Populations originating from the non-climacteric inodorus group and climacteric (e.g.…”

Section: Introductionmentioning

confidence: 99%

QTL mapping and genomic analyses of earliness and fruit ripening traits in a melon recombinant inbred lines population supported byde novoassembly of their parental genomes

Oren

Tzuri

Dafna

et al. 2022

Horticulture Research

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Earliness and ripening behavior are important attributes of fruits on and off the vine, and affect quality and preference of both growers and consumers. Fruit ripening is a complex physiological process that involves metabolic shifts affecting fruit color, firmness, and aroma production. Melon is a promising model crop for the study of fruit ripening, as the full spectrum of climacteric behavior is represented across the natural variation. Using Recombinant Inbred Lines (RILs) population derived from the parental lines “Dulce” (reticulatus, climacteric) and “Tam Dew” (inodorus, non-climacteric) that vary in earliness and ripening traits, we mapped QTLs for ethylene emission, fruit firmness and days to flowering and maturity. To further annotate the main QTL intervals and identify candidate genes, we used Oxford Nanopore long-read sequencing in combination with Illumina short-read resequencing, to assemble the parental genomes de-novo. In addition to 2.5 million genome-wide SNPs and short InDels detected between the parents, we also highlight here the structural variation between these lines and the reference melon genome. Through systematic multi-layered prioritization process, we identified 18 potential polymorphisms in candidate genes within multi-trait QTLs. The associations of selected SNPs with earliness and ripening traits were further validated across a panel of 177 diverse melon accessions and across a diallel population of 190 F1 hybrids derived from a core subset of 20 diverse parents. The combination of advanced genomic tools with diverse germplasm and targeted mapping populations is demonstrated as a way to leverage forward genetics strategies to dissect complex horticulturally important traits.

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Section: Introductionmentioning

confidence: 99%

QTL mapping and genomic analyses of earliness and fruit ripening traits in a melon recombinant inbred lines population supported byde novoassembly of their parental genomes

Oren

Tzuri

Dafna

et al. 2022

Horticulture Research

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“…Enhancing the shelf life of melons is a priority in melon breeding (Burger et al 2010;Nunez-Palenius et al 2008). Nondessert melons such as makua, conomon and acidulus melons are the genetic treasure for enhancing shelf life of dessert melons (Perpiná et al 2017; Sripongprapai and Tira-Umphon 2014). Acidulus melons of South India have exhibited the longest shelf life up to 300 days (Table 1).…”

Section: Diversity In the Underexploited Mangalore Melonmentioning

confidence: 99%

“…Crossability of Mangalore melon with cantaloupe melon offers an opportunity to enhance the shelf life of cantaloupe (Subramanian 2008), either through development of introgression lines similar to MAK-10 Charentais breeding line developed by introgression of a genomic region from Makuwa melon (Perpiná et al 2017) or through marker assisted backcrossing (Behera et al 2011). In either case, identi cation of genes/markers linked to will hasten precise transfer of shelf life genes.…”

Section: Mangalore Melons Exhibit Non-climacteric Fruit Ripeningmentioning

confidence: 99%

Genetic diversity assessment and gene expression analysis of prolonged shelf-life genes in Mangalore melon (Cucumis melo ssp. agrestis var. acidulus)

et al. 2021

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Mangalore melon (Cucumis melo ssp. agrestis var. acidulus) is a non-dessert melon, extensively grown in the coastal districts of South India, but hardly known to the rest of the World. Immature or mature fruits of Mangalore melon are used in preparation of delicious dishes such as vegetable stew, chutneys and curries. They are appreciated for nutritional values, long shelf life and biotic stress resistance. Seventy-nine accessions of Mangalore melon were collected from ve states of South India and their genetic diversity was assessed using inter simple sequence repeat (ISSR) markers. Putative candidate genes of extended shelf life in Mangalore melon were studied by quantitative reverse transcription polymerase chain reaction in comparison with cantaloupe (Cucumis melo L.). Shelf life varied from 65 days to 300 days at room temperature. Six ISSR primers ampli ed 142 fragments ranging from 80 bp to 2380 bp with an average of 23.66 bands per marker on a high-resolution capillary electrophoresis system. Neighbor joining phylogenetic tree construction from the ISSR allele similarity based genetic distance revealed two major clusters with 46 and 33 accessions in each cluster. Expression of fruit ripening related genes of ethylene biosynthesis (1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase) and cell wall metabolism (polygalacturonase, xyloglucan endotransglucosylase/hydrolase and expansin) in Mangalore melons was signi cantly lower than the cantaloupe melon at 180 days after harvest. Mangalore melon is a promising genetic resource for enhancing the shelf life of melons and the putative candidate genes are useful in enhancing shelf life of cantaloupe following validation and conformation.

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“…To better understand the effect of introgressions in LG X and the transcriptional regulation of metabolic genes during fruit ripening, other specific development and textural transcriptome analyses are necessary, probably using NILs with shorter introgressions such as SC10-1, SC10-3 or SC10-4, or the development of NILs with shorter introgressions that are more useful for melon breeding. 109,110 Recently, Perpiñá et al 111 developed a breeding line with a short introgression (between markers CMPSNP172 and CMPSNP65) in LG X from the cultivar Ginsen Makuwa (closely related to SC) 112 that also extended fruit shelf life and maintained flesh firmness, but in the Charentais genetic background (a climacteric type). This would confirm the presence of genes involved in fruit ripening in LG X from different germplasm sources the effects of which are stable in different genetic backgrounds and, therefore, with a potential for breeding.…”

Section: General Processes Deduced From Transcriptome Analysismentioning

confidence: 99%

Transcriptomic analysis of a near‐isogenic line of melon with high fruit flesh firmness during ripening

et al. 2020

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BACKGROUND: A near-isogenic line (NIL) of melon (SC10-2) with introgression in linkage group X was studied from harvest (at firm-ripe stage of maturity) until day 18 of postharvest storage at 20.5°C together with its parental control ('Piel de Sapo', PS). RESULTS: SC10-2 showed higher flesh firmness and whole fruit hardness but lower juiciness than its parental. SC10-2 showed a decrease in respiration rate accompanied by a decrease in ethylene production during ripening, both of which fell to a greater extent than in PS. The introgression affected 11 volatile organic compounds (VOCs), the levels of which during ripening were generally higher in SC10-2 than in PS. Transcriptomic analysis from RNA-Seq revealed differentially expressed genes (DEGs) associated with the effects studied. For example, 909 DEGs were exclusive to the introgression, and only 23 DEGs were exclusive to postharvest ripening time. Major functions of the DEGs associated with introgression or ripening time were identified by cluster analysis. About 37 genes directly and/or indirectly affected the delay in ripening of SC10-2 compared with PS in general and, more particularly, the physiological and quality traits measured and, probably, the differential non-climacteric response. Of the former genes, we studied in more detail at least five that mapped in the introgression in linkage group (LG) X, and 32 outside it. CONCLUSION: There is an apparent control of textural changes, VOCs and fruit ripening by an expression quantitative trait locus located in LG X together with a direct control on them due to genes presented in the introgression (CmTrpD, CmNADH1, CmTCP15, CmGDSL esterase/lipase, and CmHK4-like) and CmNAC18.

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‘MAK-10’: A Long Shelf-life Charentais Breeding Line Developed by Introgression of a Genomic Region from Makuwa Melon

Cited by 17 publications

References 22 publications

QTL mapping and genomic analyses of earliness and fruit ripening traits in a melon recombinant inbred lines population supported byde novoassembly of their parental genomes

QTL mapping and genomic analyses of earliness and fruit ripening traits in a melon recombinant inbred lines population supported byde novoassembly of their parental genomes

Genetic diversity assessment and gene expression analysis of prolonged shelf-life genes in Mangalore melon (Cucumis melo ssp. agrestis var. acidulus)

Transcriptomic analysis of a near‐isogenic line of melon with high fruit flesh firmness during ripening

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