“…Among the various synchrotron data-collection strategies that have been proposed to optimize native-SAD ( Olieric et al., 2016 ) - including sulfur-SAD - phasing, the use of a long-wavelength (2.7 Å) over 1.9 Å ( Cianci et al., 2016 ; Banerjee et al., 2016 ; Basu et al., 2019b ) was shown to be advantageous for 100 μm or smaller crystals ( Basu et al., 2019b ; Liebschner et al., 2016 ). Moreover, successful native SAD phasing was achieved in several difficult cases using low X-ray dose, high redundancy and multi-orientations data collection of multiple crystals ( Liu et al., 2012 , 2014 ; Assmann et al., 2020 ; Banerjee et al., 2016 ; Akey et al., 2014 ; El Omari et al., 2014 ; Klinke et al., 2015 ), or even a single crystal ( Weinert et al., 2015 ; Basu et al., 2019 , Basu et al., 2019 ), which obviously removes the problem of non-isomorphism between various crystals. The latest technical advances include solution-free mounting systems to minimize X-ray absorption at long-wavelengths ( Yu et al., 2020 ) and assembly of numerous partial single-crystal data sets from microcrystals with sizes less than 10 μm ( Cianci et al., 2019 ; Guo et al., 2019 ; Nass et al., 2020 ).…”