Making a hydrophoretic focuser tunable using a diaphragm

Yan, Sheng; Zhang, Jun; Chen, Huaying; Alici, Gürsel; Du, Haiping; Zhu, Yonggang; Li, Weihua

doi:10.1063/1.4903761

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…In contrast, sheathless focusing of particles relies on a force field to act directly on the suspended particles and move them laterally for alignment, which is often flexible in control and simple in operation. So far, a variety of forces have been demonstrated to focus particles in microfluidic devices, which can be either externally imposed like acoustic, 5 electric, 6 magnetic, 7 optical 8 forces, etc., or internally induced like inertial, 9 viscoelastic, [10][11][12][13] hydrodynamic, 14 and dielectrophoretic 15 forces. However, these methods often suffer from low effectiveness when working with small particles due to the strong sizedependence of nearly, if not all, every force field.…”

Section: Introductionmentioning

confidence: 99%

Viscoelastic effects on electrokinetic particle focusing in a constricted microchannel

DuBose

Joo

et al. 2015

Biomicrofluidics

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Focusing suspended particles in a fluid into a single file is often necessary prior to continuous-flow detection, analysis, and separation. Electrokinetic particle focusing has been demonstrated in constricted microchannels by the use of the constrictioninduced dielectrophoresis. However, previous studies on this subject have been limited to Newtonian fluids only. We report in this paper an experimental investigation of the viscoelastic effects on electrokinetic particle focusing in non-Newtonian polyethylene oxide solutions through a constricted microchannel. The width of the focused particle stream is found NOT to decrease with the increase in DC electric field, which is different from that in Newtonian fluids. Moreover, particle aggregations are observed at relatively high electric fields to first form inside the constriction. They can then either move forward and exit the constriction in an explosive mode or roll back to the constriction entrance for further accumulations. These unexpected phenomena are distinct from the findings in our earlier paper [Lu et al., Biomicrofluidics 8, 021802 (2014)], where particles are observed to oscillate inside the constriction and not to pass through until a chain of sufficient length is formed. They are speculated to be a consequence of the fluid viscoelasticity effects.

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Section: Introductionmentioning

confidence: 99%

Viscoelastic effects on electrokinetic particle focusing in a constricted microchannel

DuBose

Joo

et al. 2015

Biomicrofluidics

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“…Like the drawbacks existing in MP, a DEP buffer will have a similar issue because DEP‐based devices do not work very well with physiological media (conductivities >1 S/m), where cells become less polarisable than the medium , which is why they usually operate in a low conductive medium. Here, the sucrose solution typically acts as the base of a DEP buffer whose conductivity is adjusted by adding phosphate buffered saline to achieve a better separation performance .…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

Hybrid microfluidics combined with active and passive approaches for continuous cell separation

et al. 2016

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Microfluidics, which is classified as either active or passive, is capable of separating cells of interest from a complex and heterogeneous sample. Active methods utilise external fields such as electric, magnetic, acoustic, and optical to drive cells for separation, while passive methods utilise channel structures, intrinsic hydrodynamic forces, and steric hindrances to manipulate cells. However, when processing complex biological samples such as whole blood with rare cells, separation with a single module microfluidic device is difficult. Hybrid microfluidics is an emerging technique, which utilises active and passive methods whilst fulfilling higher requirements for stable performance, versatility, and convenience, including (i) the ability to process multi-target cells, (ii) enhanced ability for multiplexed separation, (iii) higher sensitivity, and (iv) tunability for a wider operational range. This review introduces the fundamental physics and typical formats for subclasses of hybrid microfluidic devices based on their different physical fields; presents current examples of cell sorting to highlight the advantage and usefulness of hybrid microfluidics on biomedicine, and then discusses the challenges and perspective of future development and the promising direction of research in this field.

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“…25 The local anisotropy of the grooves constructs rotational flows that are transverse to the main flow direction. If the groove gap satisfies the design criteria for particle ordering by hydrophoresis, 26,27 the cells can be displaced from the original streamline toward the channel wall by steric interactions. To ensure hydrophoretic ordering of the U937 cells ranged from 10 to 18 μm in diameter, we set h g = 18 μm and h t = 22 μm.…”

Section: Introductionmentioning

confidence: 99%

An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood

Lee

Jiang

et al. 2018

Biomicrofluidics

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Reliable separation and isolation of target single cells from bodily fluids with high purity is of great significance for an accurate and quantitative understanding of the cellular heterogeneity. Here, we describe a fully integrated single-blood-cell analysis platform capable of size-selective cell separation from a population containing a wide distribution of sizes such as diluted blood sample and highly efficient entrapment of single monocytes. The spiked single U937 cells (human monocyte cell line) are separated in sequence by two different-sized microfilters for removing large cell clumps, white blood cells, and red blood cells and then discriminated by dielectrophoretic force and isolated individually by downstream single-cell trapping arrays. When 2% hematocrit blood cells with a final ratio of 1:1000 U937 cells were introduced under the flow rate of 0.2 ml/h, 400 U937 cells were trapped sequentially and deterministically within 40 s with single-cell occupancy of up to 85%. As a proof-of-concept, we also demonstrated single monocyte isolation from diluted blood using the integrated microfluidic device. This size-selective, label-free, and live-cell enrichment microfluidic single blood-cell isolation platform for the processing of cancer and blood cells has a myriad of applications in areas such as singlecell genetic analysis, stem cell biology, point-of-care diagnostics, and cancer diagnostics.

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Making a hydrophoretic focuser tunable using a diaphragm

Cited by 10 publications

References 34 publications

Viscoelastic effects on electrokinetic particle focusing in a constricted microchannel

Viscoelastic effects on electrokinetic particle focusing in a constricted microchannel

Hybrid microfluidics combined with active and passive approaches for continuous cell separation

An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood

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