Making sense of luminescence from GRAS optical probes

Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

“…As mentioned before, AR contains an azo group that is presumably responsible for its molecular rotor behavior, i.e. low to undetectable emission intensity in low viscosity fluids and increased emission intensity in highly viscous environments [17].…”

“…Although there is a large Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared library with lifetime standards [6][7][8][9][10][11], only a limited number of standards with ultra-short and negligible fluorescence lifetimes are available, which can substitute the use of scatterer [12][13][14][15][16]. During our recent study on fluorescence properties of food/cosmetics azo dyes [17] we have realized that some of them have remarkable short lifetimes in low viscous solutions and can effectively be used as a substitute for scatterer.…”

Fisetin-Mediated Perturbations of Membrane Permeability and Intracellular pH in Candida albicans

“…The orange fluorescence was initially thought to be due to the pH difference, but it was found that the color was due to the autofluorescence of fisetin. It's worth noting that fisetin-treated cells exhibited a greenish appearance with distinct green cell boundaries, attributed to the green autofluorescence of fisetin, which is excited at 345 nm and emits light at 520 nm [ 30 ]. This autofluorescence of fisetin adhered to the surface of C. albicans cells may complicate data interpretation, making it challenging to distinguish between DMSO- and fisetin-treated cells in terms of fluorescence color and intensity.…”