Making Thick Paraffin Sections Thinner by Slide Shaving

Innervation of the supranasal sac of the puff adder

“…Supranasal sacs of both specimens were dissected out, dehydrated and cleared in alcohols and xylene, embedded in paraffin, and sectioned at 20 and 30 µm. A few of the 20 µm sections were later shaved to produce two sections 8 µm thick (Silver, 1996). Sections were stained with Winkelman's (1960) method for nerve endings, Kiernan's (1990) physical developer method for axons, Ehrlich's acid hematoxylin (Lillie, 1954), toluidine blue (Lillie, 1954), azure A eosinate (Lillie, 1954), PAS (Lillie, 1954), colloidal iron (Mowry, 1958), paraldehyde fuchsin and alcian blue (Spicer and Meyer, 1960), and Cason's trichrome (Kiernan, 1990).…”