Malaria vector Anopheles culicifacies sibling species differentiation using egg morphometry and morphology

Tyagi, Varun; Sharma, Akash; Dhiman, Sunil; Srivastava, AR; Yadav, Ruchi; Sukumaran, D.; Agrawal, Om P.; Veer, Vijay

doi:10.1186/s13071-016-1478-5

Cited by 13 publications

(12 citation statements)

References 34 publications

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“…2.8 DNA extraction of the silver nanoparticles-treated larvae DNA from the mosquito larvae was extracted using the reported method by Ballinger-Crabtree et al 31,32 Each adult female mosquito was ground with 200 mL lysis buffer (100 mM Tris-HCl, pH 8.0; 1% sodium dodecylsulphate; 50 mM NaCl; 50 mM EDTA). The mixture was kept immediately on ice for 15 min followed by heating at 65 C for 30 min.…”

Section: Histological Studies Of the Nanoparticle-treated Larvaementioning

confidence: 99%

Bio-nanoparticle assembly: a potent on-site biolarvicidal agent against mosquito vectors

et al. 2020

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Section: Histological Studies Of the Nanoparticle-treated Larvaementioning

confidence: 99%

Bio-nanoparticle assembly: a potent on-site biolarvicidal agent against mosquito vectors

et al. 2020

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“…Different PCR based methods have been used successfully in identification of mosquito complexes accurately (Walton et al, 1999;Chen et al, 2002;Phuc et al, 2003;Sharpe et al, 1999;Kengne et al, 2001;Singh et al, 2004;Tyagi et al, 2016). These methods are simple, precise and more accurately interpreted, hence cold be used in segregating the closely related sibling species from each other.…”

Section: Discussionmentioning

confidence: 99%

Potential malaria vectorAnopheles minimus(species A) still persisting in North East India

Tyagi

Goswami

Dhiman

et al. 2020

Preprint

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BackgroundVector borne infectious diseases affect two third of the world’s human population and cause mortality in millions each year. Malaria remains one of the major killers in the Indian sub-continent and transmitted uninterruptedly by many efficient vectors and their sibling species. In North East India (NE), Anopheles minimus has been recognized as an important vector which shares majority of malaria cases. This study primarily focuses on to recognize the presence and distribution of sibling species of An. minimus in certain endemic area of NE India.MethodsAnopheles species were collected and identified using available morphological keys. The genomic DNA was extracted from the mosquito specimen and used to perform species specific PCR (ss PCR) for molecular identification of major malaria vector An. minimus sibling speciesResultMorphological identification suggested the presence of An. minimus sl in low density in the study area. The specimen of An. minimus subjected to ss PCR confirmed the prevalence of only one sibling species namely, An. minimus A in Sialmari and Chandubi.ConclusionThough in low density, but malaria vector An. minimus is still present in certain endemic areas of NE India. The ss PCR assay employed presently suggested that An. minimus sibling species A is prevailing in the region. Presently used ss PCR assay was simpler, faster, cheaper and more readily interpreted than earlier assays. This information could be useful in understanding of current prevalence and distribution of An. minimus sibling species complex in NE region of India.

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“…; Faull & Williams ; Tyagi et al . ). There are species‐specific micropatterns formed on the surface of mosquito eggs (Hinton ; Sahlen ; Suman et al .…”

Section: Introductionmentioning

confidence: 97%

Structural characterization of the micropatterned egg plastron in the mosquito, Aedes albopictus

Kim

Sun

Bae

et al. 2020

Entomological Research

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Eggs of the Asian tiger mosquito Aedes albopictus are fully covered with an aircovering plastron network that enables them to stay below the surface of the water. Scanning electron microscopy revealed that the chorionic surface was covered with clusters of globular tubercles of different sizes. Histologically, the eggshell provides a typical water-repellent microarchitecture consisting of an outer exochorion, a membranous endochorion and an intermediate pillar layer. The eggshell has a distinct chorionic tubercle that increases the surface area for gas exchange to enhance respiration capacity. In particular, the chorionic wall gives rise to a hexagonal pattern with smooth and elevated boundaries. In A. albopictus, a set of micropatterns for each hexagon was specified, and each central tubercle was surrounded by 20 small peripheral tubercles. Our fine structural analysis revealed that the partitioning of the surface into numerous hexagonal chorionic sculptures is associated with the Voronoi partition (tessellation), based on the distance to a point in a specific area of the plane. Therefore, the micropatterned surface of the mosquito eggshell appears to not only resist wetting by hydrostatic pressure but also provide resistance to lysate deposition by the biofouling process.

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Malaria vector Anopheles culicifacies sibling species differentiation using egg morphometry and morphology

Cited by 13 publications

References 34 publications

Bio-nanoparticle assembly: a potent on-site biolarvicidal agent against mosquito vectors

Bio-nanoparticle assembly: a potent on-site biolarvicidal agent against mosquito vectors

Potential malaria vectorAnopheles minimus(species A) still persisting in North East India

Structural characterization of the micropatterned egg plastron in the mosquito, Aedes albopictus

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