Malate Decarboxylation by <i>Kalanchoë daigremontiana</i> Mitochondria and Its Role in Crassulacean Acid Metabolism

Day, David A.

doi:10.1104/pp.65.4.675

Cited by 42 publications

(37 citation statements)

References 25 publications

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“…The contributions of the two enzyme systems to malate oxidation strongly depend on the mitochondrial environment: pH (2,13,23), added NAD+ (4,20,22,23), concentration of malate (24), and level of NADH in the matrix space (18). The effects of so many factors can easily account for the variability observed in the responses of malate oxidation to rotenone (22,25,26).…”

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Interaction of Benzylaminopurine with Electron Transport in Plant Mitochondria during Malate Oxidation

Chauveau¹,

Dizengremel²,

Roussaux³

1983

Plant Physiol.

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The effect of 6-benzylaminopurine (BA) was assayed on malate oxidation in mitochondria isolated from fresh and aged potato (Solanum tuberosum L.) slices. Depending on the experimental pH, two pathways for malate oxidation were selected. A pH of 7.7 favored the activity of malate dehydrogenase, which is connected with a rotenone-sensitive NADH dehydrogenase, whereas at pH 6.5 malic enzyme, linked to a rotenone-resistant NADH dehydrogenase, was more active.Experimental results indicate the existence of two sites of inhibition for BA. The first site is common with the site of inhibition of rotenone. The second site is on the classical cyanide-resistant alternative pathway, but is different from the site of salicylhydroxamic acid (SHAM) inhibition, as in succinate oxidation.Moreover, a distinct cyanide-resistant pathway, sensitive to SHAM but resistant to BA, is found to coexist with the well-known alternative pathway which is sensitive to SHAM and BA. This outlet of electrons can accommodate 10% of the total electron flow in mitochondria from fresh slices, and up to 30% in mitochondria from aged slices.

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Interaction of Benzylaminopurine with Electron Transport in Plant Mitochondria during Malate Oxidation

Chauveau¹,

Dizengremel²,

Roussaux³

1983

Plant Physiol.

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“…The enzyme required an activator such as CoA, acetyl-Co A, fructose-1,6-bP, sulfate, or a reductant for full activity (2-4, 9, 11), although these compounds do not exert appreciable effects on the activity of intact mitochondria (2)(3)(4). The role of malic enzyme in mitochondrial substrate oxidation and respiration has been widely investigated (3,5,6,12,14,18). Recent work (2,5,6) indicates a localization in the mitochondrial matrix, similar to the homologous mammalian protein (13) and at variance with other views (3,14).…”

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confidence: 99%

“…The role of malic enzyme in mitochondrial substrate oxidation and respiration has been widely investigated (3,5,6,12,14,18). Recent work (2,5,6) indicates a localization in the mitochondrial matrix, similar to the homologous mammalian protein (13) and at variance with other views (3,14). Davies and Patil (4) have interpreted the regulation of NADmalic enzyme as connected to the control of intracellular proton activity (pH-stat), involving also PEP carboxylase.…”

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“…Davies and Patil (4) have interpreted the regulation of NADmalic enzyme as connected to the control of intracellular proton activity (pH-stat), involving also PEP carboxylase. This possibility has recently been discussed (5,17). The enzyme activity is particularly high in several plants possessing either the C4 or the CAM pathway of photosynthesis (2,5,7,9).…”

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“…This possibility has recently been discussed (5,17). The enzyme activity is particularly high in several plants possessing either the C4 or the CAM pathway of photosynthesis (2,5,7,9). In these species, malate decarboxylation represents a major source of CO2 for photosynthetic carbon assimilation.…”

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confidence: 99%

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Activation Kinetics of NAD-Dependent Malic Enzyme of Cauliflower Bud Mitochondria

Valenti¹,

Pupillo²

1981

Plant Physiol.

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NAD-dependent malic enzyme (EC 1.1.1.39) was obtained from isolated mitochondria of cauliflower buds (Brassica okracca L., var. botrytis). The NAD-linked activty is accompanied by a minor NADP-linked activity. Some contaminant NADP-malc enzyme from the supernatant and the plasma membrane is usually present in crude mitochondrial preparations.NAD-dependent malic enzyme has been purified 38-fold by ammonium sulfate fractionation and gel permeation chromatography, to a specific activity up to 2 micromoles per minute per millgram.

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Influence of Drought on Mitochondrial Activity, Photosynthesis, Nocturnal Acid Accumulation and Water Relations in the CAM Plants Prenia sladeniana(ME-type) and Crassula lycopodioides(PEPCK-type)

Herppich¹

2000

Annals of Botany

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Malate Decarboxylation by Kalanchoë daigremontiana Mitochondria and Its Role in Crassulacean Acid Metabolism

Cited by 42 publications

References 25 publications

Interaction of Benzylaminopurine with Electron Transport in Plant Mitochondria during Malate Oxidation

Interaction of Benzylaminopurine with Electron Transport in Plant Mitochondria during Malate Oxidation

Activation Kinetics of NAD-Dependent Malic Enzyme of Cauliflower Bud Mitochondria

Influence of Drought on Mitochondrial Activity, Photosynthesis, Nocturnal Acid Accumulation and Water Relations in the CAM Plants Prenia sladeniana(ME-type) and Crassula lycopodioides(PEPCK-type)

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