MALDI mass spectrometric imaging of biological tissue sections

Processing MALDI mass spectra to improve mass spectral direct tissue analysis

“…Molecular profiling and imaging experiments performed directly on tissue using MALDI mass spectrometry are methods gaining considerable popularity [1][2][3][4][5][6][7][8][9][10]. The technique is characterized by a number of valuable and useful features including minimal sample preparation, ease of use, heightened throughput, and cellular specificity.…”

“…The technique is characterized by a number of valuable and useful features including minimal sample preparation, ease of use, heightened throughput, and cellular specificity. MALDI profiling and imaging has already been successfully applied to samples ranging from individually captured cells [11,12], to intact organs, and whole animal sagittal sections [1]. Although a relatively young method, tissue analysis by imaging mass spectrometry (IMS) has been used to address a number of clinical questions in cancer [13,14], neurodegenerative diseases [15][16][17], organ development [18] and the study of drug and metabolite distribution [19][20][21].…”

Imagerie MALDI

“…Dans le cas de coupes congelées ayant été conservées plus de 6 mois, des traitements par des solvants organiques peuvent être réalisés pour éliminer la couche de lipides qui saturent la coupe [23] et ainsi obtenir un meilleur signal. De plus la possibilité de faire de la digestion trypsique in situ sur coupes fraîches [24] par le biais ou non d'un transfert sur membrane [25] devra permettre l'accès à l'information moléculaire. Cependant, la plupart des échantillons conservés par les pathologistes au sein des hôpitaux sont des tissus fixés au formaldéhyde puis inclus dans la paraffine.…”

Proteome imaging: A closer look at life's organization

“…4. This technology is now in the author's lab beginning to be employed for the direct analysis of tissues combined with a direct molecular imaging technique [21]. …”

“…Diffraction limited absorption images show the presence of the proteinaceous material in the arterial wall with approximately 6-mm spatial resolution in a 4006400-mm field of view. Imaging the Amide-band at 1650 cm 21 clearly shows the distribution of protein rich material in arteriosclerotic plaques deposited on the arterial wall. The absence of staining chemicals in this non-destructive analytical technique allows further analysis of the tissue section surface with other molecular imaging techniques.…”