“…High Performance Liquid Chromatography (HPLC) (Poonguzhali et al, 2007), Liquid Chromatography coupled with Mass Spectrometry (LC-MS) (Middleton et al, 2002), High Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-MS) (Liu et al, 2017), Liquid Chromatography coupled with tandem Mass Spectrometry (LC-MS/MS) (Ortori et al, 2011), Gas Chromatography coupled with Mass Spectrometry (GC-MS) (Cataldi et al, 2007), Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI-MS) (Kim et al, 2015), Nuclear Magnetic Resonance Spectroscopy (NMR) (Kumar et al, 2016) and Fourier Transform Infrared Spectroscopy (FTIR) (Taghadosi et al, 2015) have been explored for detection of AHLs from cell-free supernatants of gram negative bacterial cultures. Although, these detection techniques have achieved remarkable sensitivities in AHL quantification, they often require extensive sample pre-treatment and/or molecular derivatization steps as prerequisites for the analysis.…”