MALDI-Qq-TOF-MS and transient gene expression analysis indicated co-enhancement of β-1,3-glucanase and endochitinase by tMEK2 and the involvement of divergent pathways

Xing, Tim; Rampitsch, Chris; Miki, Brian; Mauthe, Wayne; Stebbing, Jo‐Ann; Malik, Kamal; Jordan, Mark C.

doi:10.1016/s0885-5765(03)00062-6

Cited by 16 publications

(8 citation statements)

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“…Overexpression of tMEK2 MUT in tomato enhanced resistance to the bacterial pathogen Pseudomonas syringae pv. tomato and activated different pathways in biotic stress responses (Xing et al 2001(Xing et al , 2003. Transgenic wheat overexpressing tomato tMEK2 MUT also exhibited enhanced resistance to leaf rust (Puccinia triticina) Fan et al 2009).…”

Section: Introductionmentioning

confidence: 99%

TaFLRS, a novel mitogen-activated protein kinase in wheat defence responses

et al. 2011

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Plants respond to biotic and abiotic stresses through the activation and coordination of various signalling pathways. The activation often requires the phosphorylation of proteins. In this study, we have identified the wheat TaFLRS MAP kinase (Fusarium and Leaf Rust Sensitive) gene that was upregulated in a wheat EST (expressed sequence tag) array analysis following a wheat-leaf rust interactive challenge. Our results demonstrate that TaFLRS is transcriptionally upregulated in incompatible interactions involving wheat and leaf rust and Fusarium graminearum, suggesting that this MAPK maybe involved in defence responses to these wheat pathogens. RT-PCR revealed that TaFLRS transcript levels are not altered by salicylic acid (SA) treatment. However, immunoprecipitation and western blotting analysis show that phosphorylation of TaFLRS at the TEY motif was enhanced by SA in the Fusarium head blight (FHB) resistant cultivar Frontana following challenge with the FHB pathogen. The role of TaFLRS MAP kinase in defence responses in wheat is discussed.

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Section: Introductionmentioning

confidence: 99%

TaFLRS, a novel mitogen-activated protein kinase in wheat defence responses

et al. 2011

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“…tMEK2 (also named LeMKK2) is a known MAPK kinase in tomato and was previously shown to regulate the expression of antifungal factors including β-1,3-glucanase and endochitinase in response to pathogen attacks. 24 2DE was used to compare wild type tomatoes and transgenic tomato carrying tMEK2 MUT , in which the tMEK2 was constitutively actived by replacing amino acid Ser-221 and Thr-226 between sub-domains VII and VIII with glutamic acid. 24 LC-MS/MS analysis revealed a group of phosphoproteins in tMEK2…”

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 99%

“…24 2DE was used to compare wild type tomatoes and transgenic tomato carrying tMEK2 MUT , in which the tMEK2 was constitutively actived by replacing amino acid Ser-221 and Thr-226 between sub-domains VII and VIII with glutamic acid. 24 LC-MS/MS analysis revealed a group of phosphoproteins in tMEK2…”

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 99%

“…38,39 Mutation cloning for in vivo expression using amino acid analogs that cannot be phosphorylated or analogs mimicking phosphorylated amino acid may further illustrate the impact of site phosphorylation on cellular, biochemical and physiological function of the protein. 24,26 By defining signal filtration and amplification more specifically, it is believed that phosphoproteomic studies will continue to play a decisive role in our understanding of plant-pathogen interactions in the years to come. duplicated ex planta.…”

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 99%

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Revealing plant defense signaling

Xing¹,

Laroche²

2011

Plant Signaling & Behavior

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“…Proteomics has thus far mostly been used to seek out under-and over-expression of proteins separated by twodimensional electrophoresis, in experiments that are comparable to nucleic acid microarray experiments in genomics (e.g., Xing et al 2003). The two-dimensional gel is in fact a protein array with molecular weight and isoelectric point dimensions, and proteins from it can usually be identified successfully by peptide mass fingerprinting or de novo sequencing (Standing 2003), in either case using a matrixassisted laser desorption ionization time-of-flight mass spectrometer.…”

Section: Proteomicsmentioning

confidence: 99%

BeyondRgenes: dissecting disease-resistance pathways using genomics and proteomics

Jordan

Cloutier

Somers

et al. 2006

Canadian Journal of Plant Pathology

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Breeding for disease resistance in crops has mainly been accomplished by incorporating single resistance (R) genes. There are advantages to quantitative resistance in terms of durability but breeding for this type of resistance is difficult. New technologies in genomics and proteomics are providing insights into disease-resistance pathways. Structural genomics can identify genomic regions that carry genes controlling these pathways and provide a means for identifying and cloning the genes involved. High-throughput molecular breeding can be used to rapidly assess a large number of lines and select for multiple-resistance quantitative trait loci. This makes breeding for complex resistance types feasible. Functional genomics can identify genes involved in the resistance pathway. By merging structural and functional genomics it will be possible to correlate complex patterns of gene expression with genomic regions and identify key elements that control entire pathways. To fully understand the pathways it is necessary to look at posttranslational modification of proteins, as this is a fundamental mechanism involved in transducing the signal from the cell membrane to the nucleus to activate the resistance. Phosphoproteomics will help to discover proteins involved in the signalling pathways.Résumé : L'amélioration génétique des cultures pour la résistance aux maladies a été principalement accomplie par l'introduction de gènes de résistance (R) individuels. La résistance quantitative présente des avantages de durabilité, mais l'amélioration pour ce type de résistance est ardue. Les nouvelles technologies en génomique et en protéomique nous permettent de jeter un regard nouveau sur les voies métaboliques de résistance aux maladies. La génomique de structure permet d'identifier les régions du génome contenant des gènes qui contrôlent ces voies et nous offre la possibilité d'identifier et de cloner les gènes impliqués. L'amélioration moléculaire à haute capacité peut être utilisée pour rapidement évaluer de nombreuses lignées et pour sélectionner en fonction de plusieurs locus de caractère quantitatif. L'amélioration pour des types de résistance complexes est ainsi rendue possible. La génomique fonctionnelle permet d'identifier des gènes impliqués dans les voies métaboliques de résistance. En réunissant les génomiques de structure et fonctionnelle, il sera possible de faire le lien entre des schèmes complexes d'expression génétique et des régions du génome, et d'identifier les éléments clés qui contrôlent des voies entières. Pour bien comprendre les voies métaboliques, il est essentiel d'examiner les modifications post-traduction des protéines puisqu'il s'agit d'un mécanisme fondamental impliqué dans la transduction du signal de la membrane cellulaire au noyau afin d'activer la résistance. La phosphoprotéomique stimulera la découverte de protéines impliquées dans les voies de signalisation.

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MALDI-Qq-TOF-MS and transient gene expression analysis indicated co-enhancement of β-1,3-glucanase and endochitinase by tMEK2 and the involvement of divergent pathways

Cited by 16 publications

References 40 publications

TaFLRS, a novel mitogen-activated protein kinase in wheat defence responses

TaFLRS, a novel mitogen-activated protein kinase in wheat defence responses

Revealing plant defense signaling

BeyondRgenes: dissecting disease-resistance pathways using genomics and proteomics

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