MALDI-TOF MS-Based Clustering and Antifungal Susceptibility Tests of Talaromyces marneffei Isolates from Fujian and Guangxi (China)

Fang, Lili; Liu, Minxue; Huang, Chen; Ma, Xiaoxuan; Zheng, Yanqing; Wu, Wenjuan; Guo, Jingjie; Huang, Jiangshan; Xu, Huanji

doi:10.2147/idr.s364439

Cited by 12 publications

(7 citation statements)

References 31 publications

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Section: Discussionsupporting

confidence: 94%

“…The overall finding of echinocandin in this study was comparatively high compared with other antifungals when tested against both forms of T. marneffei . This phenomenon is consistent with the finding of Fang et al 33 and Lei et al 34 The present findings supported the claim that echinocandins might have little to no effect on T. marneffei yeast as proposed by Fang et al 33 …”

Section: Discussionsupporting

confidence: 94%

“…The present findings supported the claim that echinocandins might have little to no effect on T. marneffei yeast as proposed by Fang et al 33 The variations in the pattern of susceptibility can be due to the unique mechanisms of action of each class of antifungal. For an instance, the polyene class of amphotericin B can disrupt fungal cell membranes via ergosterol binding, pore formation and leakage of cellular ions and eventually lead to fungal cell death.…”

supporting

confidence: 92%

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Comparison of in vitro Susceptibilities of Talaromyces marneffei in Mold and Yeast Forms in Malaysia

Tan¹,

Shuhairi²,

Ginsapu³

et al. 2023

IDR

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Purpose This study was aimed to determine minimum inhibitory concentration (MIC) differences between yeast and mold forms of T. marneffei in Malaysia. Patients and Methods Ninety-seven clinical strains of T. marneffei were received from various Malaysian hospitals from the year 2020 until 2022. Their identities were determined using microscopic, macroscopic and molecular methods. Next, the susceptibility of yeast and mold forms of each isolate against amphotericin B, itraconazole, voriconazole, posaconazole, ketoconazole, isavuconazole, terbinafine, caspofungin and micafungin were tested according to the broth microdilution according to the Clinical and Laboratory Standards Institute (CLSI) M38 and M27 guidelines. The geometric means of minimal inhibitory concentration (GM MIC), MIC 50 , and MIC 90 were determined for each antifungal. Additionally, Wilcoxon signed-rank test was used to compare the significant difference of GM MICs for each antifungal, GM MIC, MIC 50 and MIC 90 for the combined nine antifungals against different growth forms of T. marneffei . The significance was set at p <0.05. Results Micafungin had the highest GM MIC, MIC 50 and MIC 90 for mold form of T. marneffei . For yeast form, amphotericin B achieved the highest GM MIC and MIC 50 while micafungin achieved the highest MIC 90 . However, the GM MIC, MIC 50 and MIC 90 of terbinafine and azole antifungals on T. marneffei were similar to each other, namely between 0.03 and 0.60µg/mL. The difference of GM MIC of all tested antifungals except caspofungin and micafungin was insignificant. Overall, GM MIC, MIC 50 and MIC 90 of the combined nine antifungals against two growth forms were insignificant. Conclusion The findings suggested either yeast or mold form can be used in the susceptibility testing of T. marneffei against amphotericin B, itraconazole, voriconazole, posaconazole, ketoconazole, isavuconazole and terbinafine.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 94%

supporting

confidence: 92%

See 1 more Smart Citation

Comparison of in vitro Susceptibilities of Talaromyces marneffei in Mold and Yeast Forms in Malaysia

Tan¹,

Shuhairi²,

Ginsapu³

et al. 2023

IDR

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“…In 2018, Lei et al [78] observed that the YeastOne TM YO10 assay may be a viable option for susceptibility testing, particularly for the susceptibility profile of echinocandins, azoles, and amphotericin B against the yeast phase of T. marneffei. A recent publication by Fang et al [79] confirmed the observations of Lei et al [78], which observed that the yeast forms of the T. marneffei isolates are inhibited by amphotericin B and azoles (itraconazole and voriconazole with MIC 50 and MIC 90 ≤ 0.015 µg/mL). Furthermore, Fang et al [79] have shown the antifungal activity of triazoles to be superior to echinocandins and 5-fluorocytosine for the yeast phase of T. marneffei.…”

Section: Challenges and Perspectives For The Diagnosis Of Talaromycosissupporting

confidence: 61%

“…A recent publication by Fang et al [79] confirmed the observations of Lei et al [78], which observed that the yeast forms of the T. marneffei isolates are inhibited by amphotericin B and azoles (itraconazole and voriconazole with MIC 50 and MIC 90 ≤ 0.015 µg/mL). Furthermore, Fang et al [79] have shown the antifungal activity of triazoles to be superior to echinocandins and 5-fluorocytosine for the yeast phase of T. marneffei. This information may be useful to determine the presence of T. marneffei, and the evolution of the strains in terms of resistance to antifungal drugs.…”

Section: Challenges and Perspectives For The Diagnosis Of Talaromycosissupporting

confidence: 61%

An Overview of Diagnostic and Management Strategies for Talaromycosis, an Underrated Disease

Zaongo

Zhang

Chen

2023

JoF

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Underrated and neglected, talaromycosis is a life-threatening fungal disease endemic to the tropical and subtropical regions of Asia. In China, it has been reported that talaromycosis mortality doubles from 24 to 50% when the diagnosis is delayed, and reaches 100% when the diagnosis is missed. Thus, the accurate diagnosis of talaromycosis is of utmost importance. Herein, in the first part of this article, we provide an extensive review of the diagnostic tools used thus far by physicians in the management of cases of talaromycosis. The challenges encountered and the perspectives which may aid in the discovery of more accurate and reliable diagnostic approaches are also discussed. In the second part of this review, we discuss the drugs used to prevent and treat T. marneffei infection. Alternative therapeutic options and potential drug resistance reported in the contemporary literature are also discussed. We aim to guide researchers towards the discovery of novel approaches to prevent, diagnose, and treat talaromycosis, and therefore improve the prognosis for those afflicted by this important disease.

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Comparison of Zybio Kit and saponin in‐house method in rapid identification of bacteria from positive blood cultures by EXS2600 matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry system

Peras,

Mareković,

Kuliš

et al. 2024

J Mass Spectrom

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We evaluated the performance of Zybio EXS2600 matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) (Zybio Inc., Chongqing, China) for the identification of bacteria from positive blood culture (BC) bottles using Blood Culture Positive Sample Pretreatment Kit (Zybio Inc., Chongqing, China) in comparison to an in‐house saponin method. Following a positive signal by the BACTEC™ FX system, confirmation of identification was achieved using subcultured growing biomass used for MALDI‐TOF MS analysis. A total of 94 positive BC bottles with 97 bacterial isolates were analyzed. The overall identification rates at the genus and species levels for the saponin method were 89.7% (87/97) and 74.2% (72/97), respectively. With the Zybio Kit, 88.7% (86/97) and 80.4% (78/97) of microorganisms were correctly identified to the genus and species levels, respectively. The saponin method identified 65.3% (32/49) of Gram‐positive bacteria at the species level, whereas the Zybio Kit achieved a higher species‐level identification rate of 79.6% (39/49) (p = 0.1153). The saponin method with additional on‐plate formic acid extraction showed a significantly higher overall identification rate in comparison to the saponin method without that step for both genus (87.6% [85/97] vs. 70.1% [68/97], p = 0.0029) and species level (70.1% [68/97] vs. 46.4% [45/97], p = 0.0008). Identification rates of Gram‐negative bacteria showed a higher identification rate, however, not statistically significant with additional Zybio Kit protocol step on both genus (85.4% [41/48] vs. 81.3% [39/48], p = 0.5858) and species level (77.1% [37/48] vs. 75% [36/48], p = 0.8120). Zybio Kit could offer an advantage in species‐level identification, particularly for Gram‐positive bacteria. The inclusion of on‐plate formic acid extraction in the saponin method notably enhanced identification at both genus and species levels for Gram‐positive bacteria. The extended protocol provided by the Zybio Kit could potentially offer an advantage in the identification of Gram‐negative bacteria at both genus and species levels. Enhancements to the Zybio EXS2600 MALDI‐TOF instrument software database are necessary.

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MALDI-TOF MS-Based Clustering and Antifungal Susceptibility Tests of Talaromyces marneffei Isolates from Fujian and Guangxi (China)

Cited by 12 publications

References 31 publications

Comparison of in vitro Susceptibilities of Talaromyces marneffei in Mold and Yeast Forms in Malaysia

Comparison of in vitro Susceptibilities of Talaromyces marneffei in Mold and Yeast Forms in Malaysia

An Overview of Diagnostic and Management Strategies for Talaromycosis, an Underrated Disease

Comparison of Zybio Kit and saponin in‐house method in rapid identification of bacteria from positive blood cultures by EXS2600 matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry system

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