MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages

Wolters, Manuel; Rohde, Holger; Maier, Τ.; Belmar-Campos, Cristina; Franke, Gefion; Scherpe, Stefanie; Aepfelbacher, Martin; Martin, Coralie

doi:10.1016/j.ijmm.2010.06.002

Cited by 219 publications

(155 citation statements)

References 35 publications

(42 reference statements)

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“…These fresh isolates were grown on a trypticase soy agar with 5 % sheep's blood for 24 h, at which time a sample was prepared for examination by MALDI-TOF mass spectrometry using a standard procedure (Mellmann et al, 2008;Wolters et al, 2011). Briefly, a 1 ml loop of bacteria was added to 300 ml HPLC-grade water and 900 ml absolute ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…Wolters et al (2011) were able to discriminate 15 MALDI-TOF profiles from 60 MRSA clinical isolates that showed a high concordance with genetic clonal complexes based on the presence or absence of 13 mass/charge (m/z) peaks. A recent report (Schlebusch et al, 2010) from an MRSA hospital outbreak in Australia compared single nucleotide polymorphism plus binary gene typing with a composite correlation index calculated from MALDI-TOF spectra; both methods found similar inter-strain relatedness.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Staphylococcus aureus USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Boggs

Cazares

Drake

2012

Journal of Medical Microbiology

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Staphylococcus aureus strain type USA300 is an important human pathogen. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has been used successfully for rapid identification of S. aureus at the genus and species levels. Here, it was hypothesized that MALDI-TOF could be used to identify USA300 organisms at the strain level. A genetic algorithm model using ClinProTools software (Bruker Daltonics) was built using 47 isolates of USA300 S. aureus and 77 non-USA300 S. aureus isolates. Three mass/charge peaks (5932, 6423 and 6592) were found to be discriminators between the groups of isolates. The model was validated using 224 test isolates: 197 of 224 test isolates were correctly classified as 'USA300 family' or 'non-USA300'. The sensitivity of the model was 0.87, with a specificity of 0.89, positive likelihood ratio of 8.19 and negative likelihood ratio of 0.15. The three-peak intensity MALDI-TOF model designed and tested in the current study can be used to rapidly identify USA300 family S. aureus isolates with reasonable sensitivity and specificity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of a Staphylococcus aureus USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Boggs

Cazares

Drake

2012

Journal of Medical Microbiology

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“…Three recent studies have demonstrated that by applying subtle minor changes in the set-up generally used in routine diagnostics, MALDI-TOF MS allowed a reproducible discrimination of major MRSA lineages (Wolters et al, 2011), an identification of Salmonella enterica subsp. enterica serovars (Dieckmann & Malorny, 2011) and a differentiation between cfiA-negative and cfiA-positive Bacteroidis fragilis isolates (Wybo et al, 2011).…”

Section: Taxonomy and Microbial Typing Taxonomic Usefulness Of Maldi-mentioning

confidence: 99%

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Opota

Prod’hom

Greub

2017

MALDI‐TOF and Tandem MS for Clinical Microbiology

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Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.

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“…Furthermore, Shiga toxin E. coli, Salmonella serotypes, C. difficile ribotypes and Vibrio cholera phenotypes have also been identified [51][52][53][54][55].…”

Section: Species Identification In 3 H Culturementioning

confidence: 99%

Recent Technology of Clinical Microbiology; Whole Genome Sequencing (WGS) and Matrix-Assisted, Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Jeongsook¹

2017

J Microb Biochem Technol

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MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages

Cited by 219 publications

References 35 publications

Characterization of a Staphylococcus aureus USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Characterization of a Staphylococcus aureus USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Recent Technology of Clinical Microbiology; Whole Genome Sequencing (WGS) and Matrix-Assisted, Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

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