MALDI-TOF MS identification of Cimex lectularius and Cimex hemipterus bedbugs

Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia

“…A CCI match value of one represents complete correlation, whereas a CCI match value of zero represents a lack of correlation. 42 The spectra of all specimens, except those used to upgrade the database, were queried against the in-laboratory homemade MS database (Supplemental Figure 1A and B). The level of identification was determined using LSVs given by MALDI-Biotyper v. 3.0 software, corresponding to a matched degree of signal intensities of mass spectra of the query and the reference spectra.…”

MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes

“…The quality of MS spectra is impacted by several parameters such as the storage conditions, the adjustment of the extraction solution volume used for protein extraction, the grinding method, and the selected body part [ 38 ]. In the first step of our study, we tested two kinds of disrupters: both tungsten beads and glass beads have proven to be useful and effective grinding methods in previous MALDI-TOF MS studies [ 38 , 40 , 66 , 67 ]. However, glass beads were reportedly the most commonly used disrupter, as they have been used for various arthropods identification [ 39 , 40 , 66 ], for mycobacteria identification, to disrupt the mycobacterial cell wall [ 68 , 69 ], and for fungal identification, as they were most efficient than steel beads for fungal proteins extraction [ 70 ].…”

“…In the first step of our study, we tested two kinds of disrupters: both tungsten beads and glass beads have proven to be useful and effective grinding methods in previous MALDI-TOF MS studies [ 38 , 40 , 66 , 67 ]. However, glass beads were reportedly the most commonly used disrupter, as they have been used for various arthropods identification [ 39 , 40 , 66 ], for mycobacteria identification, to disrupt the mycobacterial cell wall [ 68 , 69 ], and for fungal identification, as they were most efficient than steel beads for fungal proteins extraction [ 70 ]. The glass beads also appeared to be the most suitable for snail sample homogenisation, as they provided reproducible and intense MS spectra for snail species.…”

“…The glass beads also appeared to be the most suitable for snail sample homogenisation, as they provided reproducible and intense MS spectra for snail species. As for the matrix preparation, we used alpha-cyano-4-hydroxycinnamic acid which has been successfully used for several arthropods identification [ 27 , 40 , 42 , 66 ]. As well, it has shown its performance in clinical microbiology.…”

“…This study presents a rapid, low-cost, and reliable approach for the routine identification of freshwater snail specimens including intermediate hosts of schistosomes by optimising the MALDI-TOF MS sample preparation protocol for both frozen and ethanol-preserved specimens. Recently, the MALDI-TOF MS technique has emerged in medical entomology and has proven its effectiveness in the context of identifying various species of arthropods such as fleas [29], ticks [27,[53][54][55][56], sand flies [57,58], mosquitoes [28,[59][60][61][62][63], culicoides [43], tsetse flies [64], triatomines [65] and bed bugs [66] using proteins extracted from different body parts that generate MS spectra of high quality. Furthermore, this proteomic approach has also been applied to malacology as Stephan et al were the first to report the MALDI-TOF MS use for identification of marine bivalve molluscs specifically the scallops [30].…”