MALDI-TOF MS identification of Malassezia species isolated from patients with pityriasis versicolor at the seafarers’ medical service in Dakar, Senegal

Diongue, Khadim; Kébé, O.; Faye, M.D.; Samb, D.; Diallo, Moussa; Ndiaye, Mouhamadou; Seck, Mame Cheikh; Badiane, Alpha; Ranque, Stéphane; Ndiaye, Daouda

doi:10.1016/j.mycmed.2018.09.007

Cited by 17 publications

(12 citation statements)

References 9 publications

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“…For this reason during the last five decades molecular based methods (Arendrup et al, 2013) as well as methods that identify the chemical imprint of the different species e.g., different Polymerase Chain Reaction (PCR) techniques, Matrix Assisted Laser Desorption/Ionization-Time Of Flight (MALDI-TOF) mass spectrometry (Kolecka et al, 2014;Diongue et al, 2018;Honnavar et al, 2018;Saunte et al, 2018) and or Raman spectroscopy (Petrokilidou et al, 2019) have been applied to achieve fast and accurate fungal identification.…”

Section: Diagnosticsmentioning

confidence: 99%

Malassezia-Associated Skin Diseases, the Use of Diagnostics and Treatment

Saunte

Gaitanis

Hay

2020

Front. Cell. Infect. Microbiol.

140

110

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Yeasts of the genus, Malassezia, formerly known as Pityrosporum, are lipophilic yeasts, which are a part of the normal skin flora (microbiome). Malassezia colonize the human skin after birth and must therefore, as commensals, be normally tolerated by the human immune system. The Malassezia yeasts also have a pathogenic potential where they can, under appropriate conditions, invade the stratum corneum and interact with the host immune system, both directly but also through chemical mediators. The species distribution on the skin and the pathogenetic potential of the yeast varies between different Malassezia related diseases such as head and neck dermatitis, seborrheic dermatitis, pityriasis versicolor, and Malassezia folliculitis. The diagnostic methods used to confirm the presence of Malassezia yeasts include direct microcopy, culture based methods (often a combination of morphological features of the isolate combined with biochemical test), molecular based methods such as Polymerase Chain Reaction techniques, and Matrix Assisted Laser Desorption/Ionization-Time Of Flight mass spectrometry and the chemical imprint method Raman spectroscopy. Skin diseases caused by Malassezia are usually treated with antifungal therapy and if there are associated inflammatory skin mechanisms this is often supplemented by anti-inflammatory therapy. The aim of this paper is to provide an overview of Malassezia related skin disease, diagnostic methods and treatment options.

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Section: Diagnosticsmentioning

confidence: 99%

Malassezia-Associated Skin Diseases, the Use of Diagnostics and Treatment

Saunte

Gaitanis

Hay

2020

Front. Cell. Infect. Microbiol.

140

110

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“…Pityriasis versicolor, sometimes called tinea versicolor, is a common disease worldwide, with a prevalence of up to 50% in hot and humid regions. There is no gender or ethnic predisposition [206,207]. The disease is most commonly seen in young adult to adult patients, correlating with increased sebaceous gland activity and altered lipid…”

Section: Pityriasis Versicolormentioning

confidence: 99%

“…Most patients have multiple affected areas characterized by well-demarcated, oval, hyper- or hypopigmented macules with a fine scaly surface. These lesions are variably pruritic and the neck, trunk and proximal extremities are commonly involved [ 207 , 208 , 209 ] ( Figure 3 ). Diagnosis is usually made clinically, but in individual cases, Wood’s lamp examination (coppery-orange fluorescence) or microscopic examination of fungal elements may be needed [ 6 , 210 , 211 ].…”

Section: Superficial Malassezia -Associated Diseas...mentioning

confidence: 99%

Malassezia: Zoonotic Implications, Parallels and Differences in Colonization and Disease in Humans and Animals

Hobi

Cafarchia

Romano

et al. 2022

JoF

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Malassezia spp. are commensals of the skin, oral/sinonasal cavity, lower respiratory and gastrointestinal tract. Eighteen species have been recovered from humans, other mammals and birds. They can also be isolated from diverse environments, suggesting an evolutionary trajectory of adaption from an ecological niche in plants and soil to the mucocutaneous ecosystem of warm-blooded vertebrates. In humans, dogs and cats, Malassezia-associated dermatological conditions share some commonalities. Otomycosis is common in companion animals but is rare in humans. Systemic infections, which are increasingly reported in humans, have yet to be recognized in animals. Malassezia species have also been identified as pathogenetic contributors to some chronic human diseases. While Malassezia species are host-adapted, some species are zoophilic and can cause fungemia, with outbreaks in neonatal intensive care wards associated with temporary colonization of healthcare worker’s hands from contact with their pets. Although standardization is lacking, susceptibility testing is usually performed using a modified broth microdilution method. Antifungal susceptibility can vary depending on Malassezia species, body location, infection type, disease duration, presence of co-morbidities and immunosuppression. Antifungal resistance mechanisms include biofilm formation, mutations or overexpression of ERG11, overexpression of efflux pumps and gene rearrangements or overexpression in chromosome 4.

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“…M. furfur, M. globosa and M. sympodialis are isolated most frequently from PV patients in Africa [5,28], Europe [29,30] and South America [31][32][33], respectively. In Asia, M. globosa and M. furfur are respectively predominant in Iran [34,35] and Indonesia [36], while M. globosa and M. sympodialis are predominant in India [37,38] [29,44,45], and M. globosa and M. restricta are predominant in Asia [40,[46][47][48].…”

Section: Epidemiology Of Malasseziz Infectionsmentioning

confidence: 99%

Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates

Zhang²,

Luo³

et al. 2020

Journal of Medical Microbiology

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Introduction. Malassezia folliculitis (MF) and pityriasis versicolor (PV) are common dermatoses caused by Malassezia species. Their molecular epidemiology, drug susceptibility and exoenzymes are rarely reported in China. Aim. To investigate the molecular epidemiology, drug susceptibility and enzymatic profile of Malassezia clinical isolates. Methodology. Malassezia strains were recovered from MF and PV patients and healthy subjects (HS) and identified by sequencing analysis. The minimum inhibitory concentrations (MICs) of nine antifungals (posaconazole, voriconazole, itraconazole, fluconazole, ketoconazole, miconazole, bifonazole, terbinafine and caspofungin) and tacrolimus, the interactions between three antifungals (itraconazole, ketoconazole and terbinafine) and tacrolimus, and the extracellular enzyme profile were evaluated using broth and checkerboard microdilution and the Api-Zym system, respectively. Results. Among 392 Malassezia isolates from 729 subjects (289 MF, 218 PV and 222 HS), Malassezia furfur and Malassezia globosa accounted for 67.86 and 18.88 %, respectively. M. furfur was the major species in MF and PV patients and HS. Among 60M. furfur and 50M. globosa strains, the MICs for itraconazole, posaconazole, voriconazole and ketoconazole were <1 μg ml−1. M. furfur was more susceptible to itraconazole, terbinafine and bifonazole but tolerant to miconazole compared with M. globosa (P<0.05). Synergistic effects between terbinafine and itraconazole or between tacrolimus and itraconazole, ketoconazole or terbinafine occurred in 6, 7, 6 and 9 out of 37 strains, respectively. Phosphatases, lipases and proteases were mainly secreted in 51 isolates. Conclusions. Itraconazole, posaconazole, voriconazole and ketoconazole are theagents against which there is greatest susceptibility. Synergistic effects between terbinafine and itraconazole or tacrolimas and antifungals may be irrelevant to clinical application. Overproduction of lipases could enhance the skin inhabitation of M. furfur.

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MALDI-TOF MS identification of Malassezia species isolated from patients with pityriasis versicolor at the seafarers’ medical service in Dakar, Senegal

Cited by 17 publications

References 9 publications

Malassezia-Associated Skin Diseases, the Use of Diagnostics and Treatment

Malassezia-Associated Skin Diseases, the Use of Diagnostics and Treatment

Malassezia: Zoonotic Implications, Parallels and Differences in Colonization and Disease in Humans and Animals

Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates

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