Male age is more critical to sperm DNA integrity than routine semen parameters in Chinese infertile males

Guo, Lvhua; Zhou, Hua; Liu, Min; Li, Qing; Sun, Xiaofang

doi:10.1111/and.13449

Cited by 9 publications

(8 citation statements)

References 37 publications

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“…The main characteristics of the present systematic review included 19 studies as summarized in Table 1 . There were four studies that examined the impact of APA on DNA fragmentation between Normozoospermic and subfertile males [ 21 22 23 24 ]. Three of the four studies showed a significant difference (p<0.01) in favor of APA increasing DNA fragmentation, even among normozoospermic males [ 21 22 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…There were four studies that examined the impact of APA on DNA fragmentation between Normozoospermic and subfertile males [ 21 22 23 24 ]. Three of the four studies showed a significant difference (p<0.01) in favor of APA increasing DNA fragmentation, even among normozoospermic males [ 21 22 24 ]. All six studies examining DNA fragmentation between fertile and infertile males with proven primary or secondary infertility, which reported a significant difference in favor of increasing DNA fragmentation with APA [ 25 26 27 28 29 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…Two studies examined DNA fragmentation between normozoospermic and oligoasthenoteratozoospermic males [ 37 38 ] and one study examined teratozoospermic males [ 39 ]. Six of the nineteen studies showed a significant difference (p<0.05) in favor of APA decreasing sperm motility [ 22 24 27 29 34 35 ]. Moreover, in this review three studies examined and noted the incidence of varicoceles, which there was no demonstrated effect of varicocele presence on DNA fragmentation, irrespective of APA [ 25 31 33 ].…”

Section: Resultsmentioning

confidence: 99%

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Advanced Paternal Age and Sperm DNA Fragmentation: A Systematic Review

González

Ory

Blachman‐Braun

et al. 2022

World J Mens Health

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Purpose Male ageing is often associated with defective sperm DNA remodeling mechanisms that result in poorly packaged chromatin and a decreased ability to repair DNA strand breaks. However, the impact of advanced paternal age on DNA fragmentation remains inconclusive. The aim of the present systematic review was to investigate the impact of advancing paternal age (APA) on DNA fragmentation. Materials and Methods We conducted a thorough search of listed publications in Scopus, PubMed, and EMBASE, in accordance with the PRISMA guidelines. Results We identified 3,120 articles, of which nineteen were selected for qualitative analysis, resulting in a sample of 40,668 men. Of the 19 articles evaluating the impact of APA on DFI% (DNA fragmentation Index) included, 4 were on Normozoospermic and subfertile men, 3 on normozoospermic, Oligoasthenoteratozoospermic and Teratozoospermic, 6 on fertile and infertile men, 4 on just infertile men, and 2 evaluated a general population. Seventeen of the ninrnteen studies demonstrated APA's effect and impact on DFI%. Conclusions Although there was no universal definition for APA, the present review suggests that older age is associated with increased DFI. In elderly men with normal semen parameters, further studies should be performed to assess the clinical implications of DFI, as a conventional semen analysis can often fail to detect an etiology for infertility.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Advanced Paternal Age and Sperm DNA Fragmentation: A Systematic Review

González

Ory

Blachman‐Braun

et al. 2022

World J Mens Health

View full text Add to dashboard Cite

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“…However, previous research has shown SDF has great predictive value in the field of assisted reproductive technology. Many factors affect SDF, including age [3,4], reproductive system diseases [5,6], cancer treatment [7], and smoking [8,9]. Some research has found that with aging, sperm concentration level is decreased, and the odds ratio of SDF in older men is more than double that of younger men [3,10].…”

Section: Introductionmentioning

confidence: 99%

Progress in Research on Sperm DNA Fragmentation

Qiu

et al. 2020

Med Sci Monit

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With the increasing incidence of male infertility, routine detection of semen is insufficient to accurately assess male fertility. Infertile men, who have lower odds of conceiving naturally, exhibit high levels of sperm DNA fragmentation (SDF). The mechanisms driving SDF include abnormal spermatogenesis, oxidative stress damage, and abnormal sperm apoptosis. As these factors can induce SDF and subsequent radical changes leading to male infertility, detection of the extent of SDF has become an efficient routine method for semen analysis. Although it is still debated, SDF detection has become a research hotspot in the field of reproductive medicine as a more accurate indicator for assessing sperm quality and male fertility. SDF may be involved in male infertility, reproductive assisted outcomes, and growth and development of offspring. The effective detection methods of SDF are sperm chromatin structure analysis (SCSA), terminal transferase-mediated dUTP end labeling (TUNEL) assay, single-cell gel electrophoresis (SCGE) assay, and sperm chromatin dispersion (SCD) test, and all of these methods are valuable for assisted reproductive techniques. Currently, the preferred method for detecting sperm DNA integrity is SCSA. However, the regulation network of SDF is very complex because the sperm DNA differs from the somatic cell DNA with its unique structure. A multitude of molecular factors, including coding genes, non-coding genes, or methylated DNA, participate in the complex physiological regulation activities associated with SDF. Studying SDF occurrence and the underlying mechanisms may effectively improve its clinical treatments. This review aimed to outline the research status of SDF mechanism and detection technology-related issues, as well as the effect of increased SDF rate, aiming to provide a basis for clinical male infertility diagnosis and treatment.

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“…Men older than 40 years have a higher rate of sperm fDNA (sperm fDNA index of >10%) compared to men aged <40 years (11). A retrospective study (2013-2016 years) evaluated the correlation between sperm DNA integrity and male age in 654 infertile patients: men aged ≥35 years have increased sperm fDNA rate independently of conventional sperm parameters (12). Another study indicates a statistically significant increase only after 45 years of age (13).…”

Section: Introductionmentioning

confidence: 99%

Bio-Functional Sperm Parameters: Does Age Matter?

et al. 2020

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The evaluation of biofunctional sperm parameters can explain some cases of idiopathic male infertility. Among these, sperm DNA fragmentation (fDNA) is the most studied biofunctional sperm parameter. Mitochondrial membrane potential (MMP) correlates positively with sperm motility, the evaluation of sperm apoptosis by flow cytometry allows us to identify a population of spermatozoa not recognizable at the optical microscopy and finally, lipid peroxidation (LP) and mitochondrial superoxide levels measurements are rational oxidative stress indices. Male age seems to affect sperm concentration and sperm fDNA. For these reasons, this study was undertaken to evaluate the correlation, if any, between male age and biofunctional sperm parameters evaluating their possible impact on fDNA. To accomplish this, MMP, degree of chromatin compactness, sperm apoptosis/vitality, fDNA, LP, and mitochondrial superoxide levels were evaluated by flow cytometry in a cohort of 874 men. A significant negative correlation was found between age and the percentage of alive spermatozoa (r = -0.75, p < 0.05). The percentage of spermatozoa with low MMP (L-MMP) correlated positively with the percentage of spermatozoa with abnormal chromatin compactness (r = 0.24, p < 0.05). Spermatozoa with abnormal chromatin compactness and L-MMP correlated negatively with the percentage of alive spermatozoa (r = 0.83, p < 0.05) and positively with spermatozoa with PS externalization (r = 0.13, p < 0.01). The percentage of alive spermatozoa correlated negatively with both the percentage of spermatozoa with PS externalization (r = 0.24, p < 0.01) and of the spermatozoa with fDNA (r = 0.10, p < 0.05). Spermatozoa with PS externalization correlated positively with the percentage of spermatozoa with fDNA (r = 0.09, p < 0.05). Spermatozoa with LP correlated positively with the percentage of spermatozoa with increased mitochondrial superoxide (r = 0.11, p < 0.01) In conclusion, these findings in a large number of men suggest that age, mitochondrial damage, and alteration of chromatin compactness could activate the apoptotic cascade which could result in an increased fDNA rate.

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Male age is more critical to sperm DNA integrity than routine semen parameters in Chinese infertile males

Abstract: This retrospective study evaluated the correlation between the sperm DNA integrity results and infertile male age or sperm motility in 654 infertile men undergoing infer-

Cited by 9 publications

References 37 publications

Advanced Paternal Age and Sperm DNA Fragmentation: A Systematic Review

Advanced Paternal Age and Sperm DNA Fragmentation: A Systematic Review

Progress in Research on Sperm DNA Fragmentation

Bio-Functional Sperm Parameters: Does Age Matter?

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