Male and female sex determination in hair roots

François, J; Leuven, M. Th. Matton-Van; Acosta, Juan Antonio Torres

doi:10.1111/j.1399-0004.1971.tb00258.x

Cited by 12 publications

(2 citation statements)

References 5 publications

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“…Previous studies also used to identify the presence of Y bodies in identifying the male gender, where Y bodies are predominantly seen in males. However, Klinefelter's men have both Barr and Y bodies [21]. The current study has determined the gender using samples of both contact lenses and Buccal smears purely based on Barr bodies seen during the cytological assessment.…”

Section: Discussionmentioning

confidence: 99%

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Koduri¹,

Vataparthi²,

Bolleda³

et al. 2023

Act Sci Oph

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Introduction:Identifying Barr bodies' presence has a significant diagnostic value in multifaceted science disciplines. Testing Barr bodies was important in diagnosing infertility, a syndromic association such as Klinefelter and psychopathic disorders, and disorders of sex development (DSD). It also plays a role in cancer detection in the uterine cervix, identifying transplanted retinal pigment epithelium in porcine models. Identifying the gender of victims or criminals becomes a fundamental requirement in any forensic analysis of a crime scene. The current study hypothesizes identifying the gender using Barr body detection from collected contact lens samples and tries to establish disposed soft contact lenses to consider as evidence found at the crime scene.Methods: A total of 120 (60 males and 60 females) were included in the study; from each subject, contact lens and Buccal samples were collected using sterile wooden toothpicks and soft contact lenses after insertion and removal. Both the buccal and contact lens samples were built into two smears staining with Saffranine and Methylene blue stains. The smears underwent cytological assessment by two examiners using a binocular microscope at 40X. The details of findings were graded on a scale of 1 to 5 based on visualization of Barr bodies seen. Results:The mean rank and median grading scores for higher using saffranine among females across both the samples. The sensitivity is higher at 100% for both the stains among contact lens samples, and specificity is higher among buccal samples, 93% for saffranine and 90% for methylene blue. Conclusion:Overall, it is conspicuous that contact lenses can be considered as evidence found at the crime scene in identifying the gender using Barr body detection.

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Section: Discussionmentioning

confidence: 99%

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Koduri¹,

Vataparthi²,

Bolleda³

et al. 2023

Act Sci Oph

View full text Add to dashboard Cite

show abstract

“…The general bony structure and modelling are normal for age. blood films and hair roots (Franqois et al 1971) for sex chromatin and Y fluorescent bodies (staining with quinacrine dihydrochloride, according to the method of Pearson et al (1970)). Metaphase studies were performed on the propositus, his parents, and his normal brother and sister.…”

Section: Cytogenetic Stirdiesmentioning

confidence: 99%