Male development of chromosomally female mice transgenic for Sry

Koopman, Peter; Gubbay, John; Vivian, Nigel; Goodfellow, Peter N.; Lovell-Badge, Robin

doi:10.1038/351117a0

Cited by 2,032 publications

(1,149 citation statements)

References 34 publications

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“…Since it was first isolated, SRY has rapidly been proven to be the unique Y-linked testis-determining gene through mutation studies performed in XY female patients and additive transgenic experiments in XX mice (Koopman et al, 1991). Despite its crucial role in testis differentiation, SRY is poorly conserved between mammalian species, apart from its DNA-binding HMG-box domain (Whitfield et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Montazer-Torbati

Kocer

Auguste

et al. 2010

Developmental Dynamics

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The testis-determining gene SRY is not well-conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital-ridges and not solely in pre-Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility. Developmental Dynamics 239:3324-3335,

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Section: Introductionmentioning

confidence: 99%

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Montazer-Torbati

Kocer

Auguste

et al. 2010

Developmental Dynamics

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“…Genes located on the Y chromosome, including the SRY gene in mammalian species [1][2][3] and DMY in some fish 4 , are critical for testis development. In contrast to gonadal development, most evidence suggests that the brain is masculinized during the foetal or neonatal period by the action of androgen hormones secreted from the testes, regardless of the presence of the SRY gene in brain tissue.…”

mentioning

confidence: 99%

A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras

et al. 2013

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Sexual differentiation leads to structural and behavioural differences between males and females. Here we investigate the intrinsic sex identity of the brain by constructing chicken chimeras in which the brain primordium is switched between male and female identities before gonadal development. We find that the female chimeras with male brains display delayed sexual maturation and irregular oviposition cycles, although their behaviour, plasma concentrations of sex steroids and luteinizing hormone levels are normal. The male chimeras with female brains show phenotypes similar to typical cocks. In the perinatal period, oestrogen concentrations in the genetically male brain are higher than those in the genetically female brain. Our study demonstrates that male brain cells retain male sex identity and do not differentiate into female cells to drive the normal oestrous cycle, even when situated in the female hormonal milieu. This is clear evidence for a sex-specific feature that develops independent of gonadal steroids.

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“…Sry, located on the mammalian Y chromosome, was the first sex determination gene identified in vertebrates 2, 3. Since then, several critical factors have been identified such as Sox9, Fgf9, and Dmrt1 4.…”

Section: Introductionmentioning

confidence: 99%

“…All sex determination genes examined thus far are expressed in the somatic (supporting) cells that directly surround the germ cells in the gonad 3, 4, 5, 6, 7, 8, 9, 10, 11, 12. Therefore, it is reasonable to speculate that the sexual fate of germ cells (in other words, the fate decision of germ cells to develop eggs or sperms) is triggered by the sex of the surrounding somatic cells during a normal sex determination process.…”

Section: Introductionmentioning

confidence: 99%

Germline stem cells are critical for sexual fate decision of germ cells

Tanaka

2016

BioEssays

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Egg or sperm? The mechanism of sexual fate decision in germ cells has been a long‐standing issue in biology. A recent analysis identified foxl3 as a gene that determines the sexual fate decision of germ cells in the teleost fish, medaka. foxl3/Foxl3 acts in female germline stem cells to repress commitment into male fate (spermatogenesis), indicating that the presence of mitotic germ cells in the female is critical for continuous sexual fate decision of germ cells in medaka gonads. Interestingly, foxl3 is found in most vertebrate genomes except for mammals. This provides the interesting possibility that the sexual fate of germ cells in mammals is determined in a different way compared to foxl3‐possessing vertebrates. Considering the fact that germline stem cells are the cells where foxl3 begins to express and sexual fate decision initiates and mammalian ovary does not have typical germline stem cells, the mechanism in mammals may have been co‐evolved with germline stem cell loss in mammalian ovary.

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Male development of chromosomally female mice transgenic for Sry

Cited by 2,032 publications

References 34 publications

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras

Germline stem cells are critical for sexual fate decision of germ cells

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