Male Factors Affecting the Success of Equine In Vitro Embryo Production by Ovum Pickup-Intracytoplasmic Sperm Injection in a Clinical Setting

Galli, Cesare; Colleoni, Silvia; Duchi, Roberto; Lazzari, Giovanna

doi:10.1016/j.jevs.2016.05.014

Cited by 26 publications

(11 citation statements)

References 16 publications

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“…The amount of semen is limited, is of poor quality, and has been obtained from only three bulls. This made ICSI, a routine procedure in horses, the only option that would likely lead to success 14 . We obtained samples of the sperm available from the males and tested their quality by measuring their ability to sustain pronuclei formation and initiate early cleavage in pig oocytes following ICSI.…”

Section: Resultsmentioning

confidence: 99%

Embryos and embryonic stem cells from the white rhinoceros

et al. 2018

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The northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal in the world with only two females surviving. Here we adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes with NWR spermatozoa. We show that rhinoceros oocytes can be repeatedly recovered from live SWR females by transrectal ovum pick-up, matured, fertilized by intracytoplasmic sperm injection and developed to the blastocyst stage in vitro. Next, we generate hybrid rhinoceros embryos in vitro using gametes of NWR and SWR. We also establish embryonic stem cell lines from the SWR blastocysts. Blastocysts are cryopreserved for later embryo transfer. Our results indicate that ART could be a viable strategy to rescue genes from the iconic, almost extinct, northern white rhinoceros and may also have broader impact if applied with similar success to other endangered large mammalian species.

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Section: Resultsmentioning

confidence: 99%

Embryos and embryonic stem cells from the white rhinoceros

et al. 2018

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“…In contrast, semen with an increased susceptibility to chromatin denaturation led to a decline in blastocyst development (Choi et al, 2006). Recently, an individual stallion effect on blastocyst percentage was reported (Choi et al, 2015;Galli, Colleoni, Duchi, & Lazzari, 2016); moreover, high in vivo sperm fertility does not guarantee high in vitro fertility (Galli et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Blastocyst production after intracytoplasmic sperm injection with semen from a stallion with testicular degeneration

Roels

Smits

Ververs

et al. 2018

Reprod Domestic Animals

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In horse breeding, intracytoplasmic sperm injection (ICSI) has gained interest to obtain offspring from subfertile individuals. This paper presents a case report of a stallion with severe testicular degeneration. Semen analysis showed very low motility and 83.5% of detached heads. Histology of a testicular biopsy showed severely decreased spermatogenesis, while transmission electron microscopy of the sperm cells revealed no significant abnormalities. A total of 39 oocytes were fertilized by ICSI with frozen-thawed spermatozoa of this stallion: 25 oocytes with intact spermatozoa and 24 with detached heads. When using intact sperm cells, 8 out of the 25 oocytes cleaved, and 1 developed to the blastocyst stage 9 days after ICSI. None of the oocytes injected with a detached sperm head cleaved. Studies on the paternal influence on ICSI outcome are limited in the horse and further research is needed to define which stallion factors may influence ICSI results. Here, we report the possibility to produce a blastocyst by ICSI of a stallion suffering from testicular degeneration with a poor spermiogram, as long as an intact sperm cell containing a centriole is selected.

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“…Due to the very limited access to horse oocytes, a porcine model was used for this experiment as a method to estimate the ability of zebra sperm to induce activation as it was reported previously for horses [ 26 ]. We compared the ability of zebra, horse and porcine sperm to activate and induce pronuclear formation when injected into porcine eggs (porcine-zebra, porcine-horse, and porcine-porcine ICSI embryos) and we evaluated SMARCA4 levels in pronuclei.…”

Section: Methodsmentioning

confidence: 99%