Male:Female Sex Ratio in Births Resulting from IVF According to Swim-Up Versus Percoll Preparation of Inseminated Sperm

Check, Jerome H.; Kwirenk, D.; Katsoff, D.; Press, M.; Breen, E.; Baker, A. G.

doi:10.3109/01485019408987804

Cited by 13 publications

(10 citation statements)

References 5 publications

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“…Because the swim-up technique selects motile sperm, we were interested to determine whether the isolation of motile sperm by this routine procedure alters the ratio of X-to Y-bearing sperm. However, significant clinical results by using some of these methods for skewing the sex ratio have been reported (Check et al, 1994;Check & Katsoff, 1993;Reubinoff & Schenker, 1996). In our swim-up technique without heparin the isolate fractions had a 1:1 ratio of X-to Y-bearing sperm.…”

Section: Discussionmentioning

confidence: 99%

Effect of ejaculate, bull, and a double swim-up sperm processing method on sperm sex ratio

Madrid-Bury

Fernández

Jimenéz

et al. 2003

Zygote

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Offspring gender preselection has applications of considerable economic, health and ecological interest. In this study we analysed modifications of the percentages of spermatozoa bearing Y and X chromosomes when semen samples are submitted to a double swim-up technique as a possible method for producing embryos of known sex with in vitro fertilisation protocols. As an initial experiment to provide accurate evaluation of the method we determined the possible incidence of natural deviations in the primary sex ratio between bulls or ejaculates, analysing the percentage of Y-chromosome DNA bearing spermatozoa (%Y-CDBS) with a polymerase chain reaction (PCR) amplification of X- and Y-specific fragments. Ejaculates were tested by direct semiquantitative PCR sexing and by sexing blastocysts produced in vitro with these spermatozoa. Bulls and ejaculates did not have any effect on the %Y-CDBS or on the sex ratio of embryos produced in vitro using these ejaculates. However, our double swim-up sperm preparation method produced differences in %Y-CDBS in some of the sperm fractions, suggesting that there are intrinsic differences in capacitation of X- and Y-bearing spermatozoa that might be used to produce embryos of the desired sex with in vitro fertilisation.

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Section: Discussionmentioning

confidence: 99%

Effect of ejaculate, bull, and a double swim-up sperm processing method on sperm sex ratio

Madrid-Bury

Fernández

Jimenéz

et al. 2003

Zygote

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“…Based on this affirmation, some authors described that supernatant of swim-up procedure contained more Y sperm (Check and Katsoff, 1993;Check et al, 1994). De Jonge et al (1997), MadridBury et al (2003), Yan et al (2006) and Cesari et al (2006) evaluated the swim-up supernatant and reported no difference between X-and Ybearing sperm proportion.…”

Section: Resultsmentioning

confidence: 99%

Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production

Lucio¹,

Resende

Dernowseck-Meirelles³

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y-specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P<0.05) in deviation of sex ratio when comparing the control group (45.2% females) with the other spermatozoa selection procedures (60.6% females) (P<0.05). The sperm selection methods are capable of selecting X-bearing spermatozoa without compromising the spermatozoa fertility (cleavage and blastocyst rates, 70% and 26%, respectively) and were considered relevant methods to be introduced in bovine in vitro produced embryo programs.Keywords: density gradient centrifugation, embryo sex ratio, Percoll™, swim-up RESUMO

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“…33 The sex ratio at birth is also affected by the different IVF laboratory techniques but overall it favors males. [34][35][36][37] It has been attributed to excess female embryo mortality ratio. 33 Similar observation was also reported from Japan.…”

Section: Discussionmentioning

confidence: 99%

Density of in-vitro fertilization centers and its correlation with socioeconomic factors in Maharashtra state, India

Doke¹,

Mangal²,

Gulati³

2018

Int J Community Med Public Health

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Background: The infertility due to various reasons is increasing and correspondingly the number in vitro fertilization (IVF) centers is also increasing. Some procedures in these centers can deliver desired sex of the unborn child. The gender discrimination in India has already resulted unfavorable child sex ratio for girls. Maharashtra is one of the badly affected states. It is feared that the child sex ratio may further decline due to IVF centers. The study was carried out to measure correlation between number of IVF centers with various indicators of sex ratio infertility, and some socio-economic factors.Methods: The study was conducted in 2014 using secondary data. District wise internet search by using words ‘In-Vitro Fertilization center’ and ‘infertility treatment center’ was carried out. Whenever IVF center/procedure was noticed on the internet, the center with its address was included in that district list.Results: There was positive correlation between IVF centers and urbanization, per capita income, female literacy and negative correlation with population sex ratio and no correlation with sex ratio at birth or child sex ratio. The best correlation was observed with urbanization, which explains the observed correlation with other variables.Conclusions: The number of IVF centers is not related with magnitude of infertility and not affecting sex ratio whereas IVF density seems to be an outcome of extent of urbanization.

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Male:Female Sex Ratio in Births Resulting from IVF According to Swim-Up Versus Percoll Preparation of Inseminated Sperm

Cited by 13 publications

References 5 publications

Effect of ejaculate, bull, and a double swim-up sperm processing method on sperm sex ratio

Effect of ejaculate, bull, and a double swim-up sperm processing method on sperm sex ratio

Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production

Density of in-vitro fertilization centers and its correlation with socioeconomic factors in Maharashtra state, India

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