Male Gametophyte Development

Twell, David

doi:10.1007/978-3-642-02301-9_12

Cited by 15 publications

(14 citation statements)

References 78 publications

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“…In flowering plants, the pollen grains produced during the reproductive growth stage harbor haploid male germ cells, which are vital for sexual reproduction. Pollen development takes place in the anther locules and can be divided into two major phases: microsporogenesis and microgametogenesis [ 54 ]. During microsporogenesis, the primary sporogenous cells produce the microsporocytes and then undergo meiosis to give rise to tetrads of haploid microspores enclosed and separated by thick callosal walls, which are subsequently released as individual microspores by callose enzyme [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

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BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

Xiong

Liu

et al. 2020

IJMS

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Chinese cabbage (Brassica campestris) is an economically important leaf vegetable crop worldwide. Mounting studies have shown that cysteine-cysteine-cysteine-histidine (CCCH) zinc-finger protein genes are involved in various plant growth and development processes. However, research on the involvement of these genes in male reproductive development is still in its infancy. Here, we identified 11 male fertility-related CCCH genes in Chinese cabbage. Among them, a pair of paralogs encoding novel non-tandem CCCH zinc-finger proteins, Brassica campestris Male Fertility 30a (BcMF30a) and BcMF30c, were further characterized. They were highly expressed in pollen during microgametogenesis and continued to express in germinated pollen. Further analyses demonstrated that both BcMF30a and BcMF30c may play a dual role as transcription factors and RNA-binding proteins in plant cells. Functional analysis showed that partial bcmf30a bcmf30c pollen grains were aborted due to the degradation of pollen inclusion at the microgametogenesis phase, and the germination rate of viable pollen was also greatly reduced, indicating that BcMF30a and BcMF30c are required for both pollen development and pollen germination. This research provided insights into the function of CCCH proteins in regulating male reproductive development and laid a theoretical basis for hybrid breeding of Chinese cabbage.

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Section: Discussionmentioning

confidence: 99%

“…To date, it is expected that near 100 genes affecting pollen development and cellular functions have been isolated in Arabidopsis by several genetic screening strategies [ 54 , 57 ]. The phenotypic analyses of certain gametophytic mutants indicate that some genes are specifically involved in cell division and patterning.…”

Section: Discussionmentioning

confidence: 99%

BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

Xiong

Liu

et al. 2020

IJMS

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“…In addition to these comprehensive gene expression profiles, the functions of at least 37 genes expressed in male gametophyte involved in pollen development in Arabidopsis have been identified by mutant analysis (Twell et al, 2006;Twell, 2010). For example, loss of function of the GLC-6-P TRANSLOCATOR1, the reversibly glycosylated polypeptides RPG1 and RPG2 for polysaccharide biosynthesis, the cellulose synthases CESA1 to CESA3, CESA6, and CESA9, the long-chain bases LCB1, LCB2A, and LCB2C for sphingolipid synthesis, or the glucan synthases GSL8 and GSL10 cause male gametophytic lethality during pollen development (Niewiadomski et al, 2005;Drakakaki et al, 2006;Persson et al, 2007;Teng et al, 2008;Töller et al, 2008).…”

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confidence: 99%

COLLAPSED ABNORMAL POLLEN1Gene Encoding the Arabinokinase-Like Protein Is Involved in Pollen Development in Rice

et al. 2013

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We isolated a pollen-defective mutant, collapsed abnormal pollen1 (cap1), from Tos17 insertional mutant lines of rice (Oryza sativa). The cap1 heterozygous plant produced equal numbers of normal and collapsed abnormal grains. The abnormal pollen grains lacked almost all cytoplasmic materials, nuclei, and intine cell walls and did not germinate. Genetic analysis of crosses revealed that the cap1 mutation did not affect female reproduction or vegetative growth. CAP1 encodes a protein consisting of 996 amino acids that showed high similarity to Arabidopsis (Arabidopsis thaliana) L-arabinokinase, which catalyzes the conversion of L-arabinose to L-arabinose 1-phosphate. A wild-type genomic DNA segment containing CAP1 restored mutants to normal pollen grains. During rice pollen development, CAP1 was preferentially expressed in anthers at the bicellular pollen stage, and the effects of the cap1 mutation were mainly detected at this stage. Based on the metabolic pathway of L-arabinose, cap1 pollen phenotype may have been caused by toxic accumulation of L-arabinose or by inhibition of cell wall metabolism due to the lack of UDP-L-arabinose derived from L-arabinose 1-phosphate. The expression pattern of CAP1 was very similar to that of another Arabidopsis homolog that showed 71% amino acid identity with CAP1. Our results suggested that CAP1 and related genes are critical for pollen development in both monocotyledonous and dicotyledonous plants.

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“…Male gametophyte gene expression can be divided into two major phases: early and late (Mascarenhas, 1990). The majority of highly expressed genes in the late phase show pollen-specific expression patterns, among which numerous genes have been proved to contribute to microspore development (Twell, 2010). Our previous study showed that BcMF18 was highly abundant in the fertile flower buds but silenced in sterile ones of B. campestris GMS line 'Bcajh97-01A/B' (Huang et al, 2008a).…”

Section: Discussionmentioning

confidence: 99%

“…A considerable number of cell-wall metabolism-related genes from both the microspore and the sporophytic tissues, including the tapetum, have been identified to be implicated in the extremely intricate and precise process of pollen wall development (Twell, 2010;Ariizumi and Toriyama, 2011;Dong et al, 2013;Hsiao et al, 2015;Shi et al, 2015;Sumiyoshi et al, 2015;Hafidh et al, 2016;Xu et al, 2016). Arabinogalactan proteins (AGPs) are extensively glycosylated hydroxyproline-rich glycoproteins, ubiquitous in the plant kingdom (Showalter, 2001;Dong et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The distinct functions of two classical arabinogalactan proteins BcMF8 and BcMF18 during pollen wall development in Brassica campestris

et al. 2018

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Arabinogalactan proteins (AGPs) are extensively glycosylated hydroxyproline-rich glycoproteins ubiquitous in all plant tissues and cells. AtAGP6 and AtAGP11, the only two functionally known pollen-specific classical AGP encoding genes in Arabidopsis, are reported to have redundant functions in microspore development. BcMF18 and BcMF8 isolated from Brassica campestris are the orthologues of AtAGP6 and AtAGP11, respectively. In contrast to the functional redundancy of AtAGP6 and AtAGP11, single-gene disruption of BcMF8 led to deformed pollen grains with abnormal intine development and ectopic aperture formation in B. campestris. Here, we further explored the action of BcMF18 and its relationship with BcMF8. BcMF18 was specifically expressed in pollen during the late stages of microspore development. Antisense RNA transgenic lines with BcMF18 reduction resulted in aberrant pollen grains with abnormal cellulose distribution, lacking intine, cytoplasm and nuclei. Transgenic plants with repressive expression of both BcMF8 and BcMF18 showed a hybrid phenotype, expressing a mixture of the phenotypes of the single gene knockdown plant lines. In addition, we identified functional diversity between BcMF18/BcMF8 and AtAGP6/AtAGP11, mainly reflected by the specific contribution of BcMF18 and BcMF8 to pollen wall formation. These results suggest that, unlike the orthologous genes AtAGP6 and AtAGP11 in Arabidopsis, BcMF18 and BcMF8 are both integral to pollen biogenesis in B. campestris, acting through independent pathways during microspore development.

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Male Gametophyte Development

Cited by 15 publications

References 78 publications

BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

COLLAPSED ABNORMAL POLLEN1Gene Encoding the Arabinokinase-Like Protein Is Involved in Pollen Development in Rice

The distinct functions of two classical arabinogalactan proteins BcMF8 and BcMF18 during pollen wall development in Brassica campestris

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