Male Genital Tract Inflammation Associated with Increased Numbers of Potential Human Immunodeficiency Virus Host Cells in Semen*/Entzündung im Bereich des männlichen Genitaltraktes assoziiert mit erhöhten Zahlen von potentiellen HIV-Wirtszellen im Sperma

Wolff, Hans; Anderson, Deborah J.

doi:10.1111/j.1439-0272.1988.tb00712.x

Cited by 48 publications

(3 citation statements)

References 14 publications

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“…Olivier et al (17), Linge et al (18) and Politch et al (19) reported consistently higher concentrations of inflammatory cytokines in the semen than the blood, irrespective of HIV-infection status (20), reflecting a persistent and primed state of immune activation conducive to HIV infection. Studies confirmed that the cytokine and chemokine seminal plasma milieu supports active viral replication through ongoing activation of target CD4 T cells in situ (13, 14, 20). Higher levels of inflammatory cytokines were associated with increased HIV shedding in the genital tract, increasing the risk of transmission to sexual partners (21).…”

Section: Introductionmentioning

confidence: 80%

“…Genital inflammation, defined as a specific profile of inflammatory cytokines (10–12), increased T-cell activation and HIV target cell recruitment (13, 14), has been identified as a significant risk factor for HIV acquisition in women (10, 15, 16). Whether the definition of genital tract inflammation is gender biased, remains less well-described.…”

Section: Introductionmentioning

confidence: 99%

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Semen IgM, IgG1, and IgG3 Differentially Associate With Pro-Inflammatory Cytokines in HIV-Infected Men

et al. 2019

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Genital inflammation significantly increases the risk for HIV infection. The seminal environment is enriched in pro-inflammatory cytokines and chemokines. Here, we investigated the interplay between semen cytokines and humoral immunity to understand whether the characteristics of semen antibodies are associated with genital inflammation. In 36 HIV-infected and 40 HIV-uninfected mens' semen, HIV-specific antibodies (gp120, gp41, p66, and p24), immunoglobulin (Ig) subclasses, isotypes and cytokines, using multiplex assays, were measured. Semen IgG1, IgG3, and IgM were significantly higher in HIV-infected compared to HIV-uninfected men (p < 0.05). In HIV-uninfected men, pro-inflammatory cytokines IL-6, IL-8, and MCP-1 significantly correlated with IgG1 and total IgG (IgG1+IgG2+IgG3+IgG4) (both r≥0.55; p≤0.001). Total IgG in HIV-infected men correlated to HIV-specific antibodies in the semen irrespective of antiretroviral (ARV) use. In HIV-infected, ARV-treated men, p66 and gp41-specific antibodies were inversely correlated with IL-6 and MIP-1α (both r≥−0.65, p≤0.03). In HIV-infected, ARV-naïve men, p24 and gp120-specific antibodies correlated significantly with pro-inflammatory TNF-α (r≥0.44, p≤0.03), while p24 antibodies correlated significantly with chemokine MIP-1β (r = 0.45; p = 0.02). Local cytokines/chemokines were associated with the mucosal-specific Ig subclasses which likely effect specific antibody functions. Together, these data inform on mucosal-specific immunity that may be elicited in the male genital tract (MGT) in future vaccines and/or combination HIV prevention strategies.

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Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Semen IgM, IgG1, and IgG3 Differentially Associate With Pro-Inflammatory Cytokines in HIV-Infected Men

et al. 2019

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“…To establish an infection via the genital mucosa, HIV-1 in semen must overcome substantial immunological and physiological barriers to cross the epithelium and infect underlying target cells. Several factors are known to affect semen infectivity and enhance the risk of HIV-1 transmission to a partner, such as concomitant genital infections and inflammation, natural antimicrobial molecules (β-defensins, secretory leukocytes peptidase inhibitor -SLPI-, lysozyme, lactoferrin), semen-derived amyloid fibrils (10,11), cytokines (IL-7) (12), and male circumcision (13)(14)(15)(16)(17). However, very little is known about the actors of the innate and HIV-specific adaptive immune response present in semen that may limit or enhance viral transmission.…”

Section: Introductionmentioning

confidence: 99%

Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission

et al. 2020

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HIV-1 infection is transmitted primarily by sexual exposure, with semen being the principal contaminated fluid. However, HIV-specific immune response in semen has been understudied. We investigated specific parameters of the innate, cellular, and humoral immune response that may affect semen infectivity in macaques infected with SIVmac251. Serial semen levels of cytokines and chemokines, SIV-specific antibodies, neutralization, and FcγR-mediated functions and SIV-specific T-cell responses were assessed and compared to systemic responses across 53 cynomolgus macaques. SIV infection induced an overall inflammatory state in the semen. Several pro-inflammatory molecules correlated with SIV virus levels. Effector CD8 + T cells were expanded in semen upon infection. SIV-specific CD8 + T-cells that expressed multiple effector molecules (IFN-γ + MIP-1β + TNF +/−) were induced in the semen of a subset of SIV-infected macaques, but this did not correlate with local viral control. SIV-specific IgG, commonly capable of engaging the FcγRIIIa receptor, was detected in most semen samples although this positively correlated with seminal viral load. Several inflammatory immune responses in semen develop in the context of higher levels of SIV seminal plasma viremia. These inflammatory immune responses could play a role in viral transmission and should be considered in the development of preventive and prophylactic vaccines.

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Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: A report from the HIV heterosexual transmission study

Denny¹,

Skurnick²,

Garcia³

et al. 1996

Cytometry

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The purpose of this study was to determine the types and distribution of immune subsets present in semen from human immunodeficiency virus (HIV)‐infected (HIV+) individuals and to compare these values with those measures in semen from HIV‐negative (HIV−) individuals. To accomplish this, a direct three‐color monoclonal antibody labeling technique was employed to identify immune cells in fresh ejaculates. Once labeled, the percent of each immune subset present in the ejaculate was determined by flow cytometric analysis. The percent of CD3+ cells present in the semen of the HIV+ group showed no significant difference when compared with semen from the HIV− group. Analysis of the CD4+ subset yielded a significantly lower percent in the HIV+ group than in the HIV− group. The analysis of the CD8+ subset yielded a higher percent of cells present in semen from HIV+ individuals. The CD8 higher value along with lower CD4 value results in a lower CD4/CD8 ratio in the HIV+ group. Further subset studies showed that the percent of cells expressing naive (CD4+CD45RA+) and memory (CD4+CD45RO+) markers was lower in the HIV+ group. This study provides additional data supporting the utility of flow cytometry and monoclonal antibodies to immunophenotypic cells present in semen ejaculates. It is also the first reported application of the technique to a disease‐based model and may be useful to better understand issues of mucosal immunity and transmission of sexually transmitted diseases such as HIV. © 1996 Wiley‐Liss, Inc.

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Male Genital Tract Inflammation Associated with Increased Numbers of Potential Human Immunodeficiency Virus Host Cells in Semen*/Entzündung im Bereich des männlichen Genitaltraktes assoziiert mit erhöhten Zahlen von potentiellen HIV-Wirtszellen im Sperma

Cited by 48 publications

References 14 publications

Semen IgM, IgG1, and IgG3 Differentially Associate With Pro-Inflammatory Cytokines in HIV-Infected Men

Semen IgM, IgG1, and IgG3 Differentially Associate With Pro-Inflammatory Cytokines in HIV-Infected Men

Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission

Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: A report from the HIV heterosexual transmission study

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