Male germ cell transplantation in livestock

Hill, Jonathan R.; Dobrinski, Ina

doi:10.1071/rd05123

Cited by 72 publications

(44 citation statements)

References 55 publications

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“…By contrast, in mammalian systems designed to transplant germ cells into seminiferous tubules in postnatal recipients, the donor cells c a n n o t s u r v i v e f o r l o n g p e r i o d s i n i m m u n o c om pe te n t x e n o g e n e i c r e c i p ie nt s . Although xenogeneic germ-cell transplantation has considerable advantages (as described above), especially for use in domestic animals, such a system has so far only been developed in rodents [6][7][8][9]. Moreover, the rodent studies showed that xenogeneic spermatogenesis could only be intense Gfp, but this activity is reduced as they differentiate.…”

Section: Germ-cell Transplantation Systems In Fish Versus Other Animalsmentioning

confidence: 99%

“…T h e spermatogonial transplantation approach has been widely employed, as it is the only reliable functional assay for identifying spermatogonial stem cells in mice; these can be distinguished from s p e r m a t o g o n i a t h a t a r e c o m m i t t e d t o differentiation only by their ability to self-renew, due to the lack of definitive markers for such cells [5]. These advantages have led to the technique being applied to various types of animal, including goats, pigs, cattle and primates [6][7][8][9], and most recently to chickens [10,11].…”

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confidence: 99%

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Manipulation of Fish Germ Cell: Visualization, Cryopreservation and Transplantation

et al. 2006

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Abstract. Germ-cell transplantation has many applications in biology and animal husbandry, including investigating the complex processes of germ-cell development and differentiation, producing transgenic animals by genetically modifying germline cells, and creating broodstock systems in which a target species can be produced from a surrogate parent. The germ-cell transplantation technique was initially established in chickens using primordial germ cells (PGCs), and was subsequently extended to mice using spermatogonial stem cells. Recently, we developed the first germ-cell transplantation system in lower vertebrates using fish PGCs and spermatogonia. During mammalian germ-cell transplantation, donor spermatogonial stem cells are introduced into the seminiferous tubules of the recipient testes. By contrast, in the fish germ-cell transplantation system, donor cells are microinjected into the peritoneal cavities of newly hatched embryos; this allows the donor germ cells to migrate towards, and subsequently colonize, the recipient genital ridges. The recipient embryos have immature immune systems, so the donor germ cells can survive and even differentiate into mature gametes in their allogeneic gonads, ultimately leading to the production of normal offspring. In addition, implanted spermatogonia can successfully differentiate into sperm and eggs, respectively, in male and female recipients. The results of transplantation studies in fish are improving our understanding of the development of germ-cell systems during vertebrate evolution.

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Section: Germ-cell Transplantation Systems In Fish Versus Other Animalsmentioning

confidence: 99%

mentioning

confidence: 99%

Manipulation of Fish Germ Cell: Visualization, Cryopreservation and Transplantation

et al. 2006

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“…Primordial germ cell (PGC) transplantation technology was first applied successfully in chickens [79], and has since been used in chickens, goats, and pigs [80][81][82]. In fish, Takeuchi et al [83] developed the first PGC transplantation system in 2003.…”

Section: Transplantation Of Germline Stem Cells and Germ Cellsmentioning

confidence: 99%

Development and application of biological technologies in fish genetic breeding

Duan

Xiao

et al. 2015

Sci. China Life Sci.

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Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties. For the purpose of genetic improvement, researchers can select for desirable genetic traits, integrate a suite of traits from different donors, or alter the innate genetic traits of a species. These improved varieties have, in many cases, facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield. In this review, we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies (containing traditional selective breeding, molecular marker-assisted breeding, genome-wide selective breeding and breeding by controlling single-sex groups), integration breeding technologies (containing cross breeding, nuclear transplantation, germline stem cells and germ cells transplantation, artificial gynogenesis, artificial androgenesis and polyploid breeding) and modification breeding technologies (represented by transgenic breeding) in fish genetic breeding. Additionally, we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish, including distant hybridization, gynogenesis, and androgenesis. Finally, we systematically summarize the research status and known problems associated with each technology. fish genetic breeding, genetic improvement, biological method, traits, new variety Citation:

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“…The large scale culture of the donor germ line stem cells would provide a renewable source of stem cells for transplantation. This system would create an alternative to artificial insemination (AI) for the use of elite sires in the cattle industry in areas where AI is not practicable (Hill & Dobrinski 2006). Although AI is the most commonly used breeding technique for this purpose, its use in extensive beef cattle grazing systems is limited by the logistics involved in handling cattle during the AI procedures.…”

Section: Introductionmentioning

confidence: 99%

Successful transplantation of bovine testicular cells to heterologous recipients

Herrid¹,

Vignarajan²,

Davey³

et al. 2006

Reproduction

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While heterologous germ cell transplantation was successful in pigs and goats, autologous transplantation alone has been reported to result in donor-derived spermatogenesis in cattle. The objective of this study was to investigate whether the transplantation of heterologous germ cells could result in colonization of recipient testes in cattle of different breeds. Testicular cells were isolated from 8 Bos taurus donor bull calves and then transferred into 15 Bos indicus-cross bull calves. All animals were pre-pubertal, donors were aged 5-7 months and recipients 5-11 months, and scrotal circumferences ranged from 15 to 22 cm. Single cell suspensions of donor testicular cells, prepared by enzymatic digestion, were labelled with fluorescent dyes PKH26 or CFDA-SE, before transfer into the rete testis of recipients under ultrasonographic guidance. To assess the longevity of colonization by donor cells, recipients were castrated 2-30 weeks after cell transfer. Donor cells were observed in 15/25 (60%) of the testes that received PKH26-labelled cells, whereas no CFDA-SE-positive cell was identified in any recipients. The maturity of the donors or recipients (measured by scrotal circumference) did not affect colonization potential. In freshly isolated tubules, clumps of PKH26-positive cells were observed, which indicated either cell division or extensive local colonization of specific areas of the tubules. In frozen sections, PKH26-positive cells were identified on the seminiferous tubule basement membrane, which indicated that these cells had successfully migrated from the tubule lumen and were likely to be spermatogonia. We conclude that PKH26 was more suitable for labelling donor testis cells and donor cells can be identified up to 6 months following transfer. These results indicate that allogeneic transplantation of testicular cells can occur between Bos taurus and Bos indicus cattle. Further studies will investigate functionality of transferred testicular cells.

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Male germ cell transplantation in livestock

Cited by 72 publications

References 55 publications

Manipulation of Fish Germ Cell: Visualization, Cryopreservation and Transplantation

Manipulation of Fish Germ Cell: Visualization, Cryopreservation and Transplantation

Development and application of biological technologies in fish genetic breeding

Successful transplantation of bovine testicular cells to heterologous recipients

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