Male labial gland secretions and mitochondrial DNA markers support species status of Bombus cryptarum and B. magnus (Hymenoptera, Apidae)

Bertsch, Andreas; Schweer, Horst; Titze, Andreas; Tanaka, Hiroyuki

doi:10.1007/s00040-004-0761-1

Cited by 69 publications

(111 citation statements)

References 14 publications

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“…B. magnus and B. cryptarum have been regarded as subspecies of B. lucorum and are often referred to collectively as the 'lucorum complex' or simply synonymized to B. lucorum (Benton, 2006;Edwards and Jenner, 2005). Recent studies using CO1 barcode analysis show discrete differences between the three species Murray et al, 2008;Williams et al, 2012), in accordance with studies of labial gland secretions (Bertsch et al, 2005). Diagnostic morphological characters have also been previously reported for queens, but some of these have now been demonstrated to overlap considerably, and vary along a continuum, thus making them unreliable and leading to a high potential for misidentification .…”

Section: Introductionsupporting

confidence: 81%

Revealing the hidden niches of cryptic bumblebees in Great Britain: Implications for conservation

Scriven

Woodall

Tinsley

et al. 2015

Biological Conservation

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a b s t r a c tBumblebees are ecologically and economically important, and some species have suffered dramatic population declines. The absence of morphological diagnostic characters for the identification of some species creates difficulties for basic ecological studies, and for conservation management. The widespread and commercially exploited bumblebee subgenus Bombus sensu stricto contains a cryptic species complex, known as the lucorum complex, which in Europe comprises B. lucorum, B. cryptarum and B. magnus. Little is known about these species and much of what has been reported is likely to have suffered from incorrect identification. Although the lucorum complex as a whole is common in Great Britain, we aimed to determine whether the populations of the individual species are vulnerable and require conservation action. Using genetic methods to distinguish them, we determined the geographic distribution and abundance of the lucorum complex species in Great Britain, and assessed the extent of niche differentiation between these species. We detected major differences in the geographic range, forage use and sensitivity to summer temperatures of the three species. Bombus lucorum was found to have the broadest distribution and diet, being present throughout mainland Great Britain, whereas B. cryptarum and B. magnus were absent from large areas of central and southern England. Bombus cryptarum and B. magnus were more likely to be found at sites with lower summer temperatures. Bombus magnus, the least abundant species, was found to exhibit an unusually tight biotope association with heathland habitat. This has conservation implications for B. magnus given the current threats to this habitat type.

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Section: Introductionsupporting

confidence: 81%

Revealing the hidden niches of cryptic bumblebees in Great Britain: Implications for conservation

Scriven

Woodall

Tinsley

et al. 2015

Biological Conservation

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“…While existence of the species B. terrestris and B. lucorum has previously been generally accepted, B. magnus and B. cryptarum were considered to be merely morphological variants of the B. lucorum species (Williams, 1991(Williams, , 1998. Not until recent studies based on molecular techniques and chemical analysis of labial glands for male pheromones (Bertsch et al, 2004(Bertsch et al, , 2005 has it been shown that these are indeed separate species. Generic classifi cation of the bumblebees B. cryptarum and B. magnus on the basis of morphological characteristics, however, remains very complicated.…”

mentioning

confidence: 99%

“…Generic classifi cation of the bumblebees B. cryptarum and B. magnus on the basis of morphological characteristics, however, remains very complicated. This problem has been satisfactorily resolved only for the queens of these bumblebees (Bertsch et al, 2004(Bertsch et al, , 2005. Species identifi cation of males and workers is very diffi cult and not always reliable, as to date there are no fully applicable identifi cation keys (AMIET, 1996).…”

mentioning

confidence: 99%

Notes on distribution of Bombus cryptarum (Hymenoptera, Apoidea) in Moravian territory (Czech Republic) and its laboratory rearing

Bučánková¹,

Komzáková²,

Cholastová³

et al. 2014

Acta Univ. Agric. Silvic. Mendelianae Brun.

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BUČÁNKOVÁ, A., KOMZÁKOVÁ, O., CHOLASTOVÁ, T., PTÁČEK, V.: Notes on distribution of Bombus cryptarum (Hymenoptera, Apoidea) in Moravian territory (Czech Republic) and its laboratory rearing. Acta univ. agric. et silvic. Mendel. Brun., 2011, LIX, No. 6, pp. 69-74 B. cryptarum and B. magnus are among the so-called cryptic species whose identifi cation is very diffi cult due to high interspecifi c variability of their morphological characteristics. This greatly limits possibilities for studying their biology, bionomics and ecology. The aim of this research was to contribute to knowledge about distribution of B. cryptarum in Moravia (Czech Republic) and to verify the possibility of its laboratory rearing. During 2006During -2009 collections were performed at Moravian localities. Of the 17 specimens that were assigned to B. cryptarum or B. aff . cryptarum based on morphology, the molecular analysis of mtDNA RFLP confi rmed the identifi cation of 10 speciemens. The molecular analysis even assigned to B. cryptarum one specimen determined morphologically as B. aff . magnus. Of four queens captured in spring, and while applying a laboratory method used for breeding of B. terrestris, two complete nests were successfully reared, including queens of the 2nd generation that then set up their own brood. Species identifi cation of these queens as B. cryptarum was confi rmed by both molecular analysis and analysis of the marking pheromones of males among their off spring. A single B. terrestris worker was able to raise young queens of B. cryptarum of the brood.

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“…This is an impediment to understanding the taxonomy and systematics of bumblebees, such as revealing new species. For example, Bombus (Bombus ) cryptarum Fabricius, 1775, a common and widespread species, remained undetected until 2005 due to the high morphological similarity to B. lucorum (Bertsch et al 2005;Murray et al 2008). According to Williams et al (2012), Bombus s. str.…”

Section: Discussionmentioning

confidence: 99%

An integrative approach identifies a new species of bumblebee (Hymenoptera: Apidae: Bombini) from northeastern Brazil

2015

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-Here, we describe a new species of genus Bombus Latreille, 1802 from northeastern Brazil, Bombus (Thoracobombus ) applanatus Oliveira, Françoso & Arias, sp. nov. Molecular analysis was initially performed to confirm the new species placement within the genus Bombus . Afterward, we performed an integrative approach combining molecular data (DNA barcoding and two nuclear regions) and morphology to confirm its taxonomic status. The genetic and morphological data were very consistent and congruent supporting this group as a new species. B . (Thoracobombus ) applanatus Oliveira, Françoso & Arias, sp. nov. has the body totally covered by black pilosity and can be distinguished from closer and/or sympatric species by clypeus shape, which has a distinct flattened dorsal platform, by length of malar area and the length and shape of the hairs, shorter and aligned as if it was trimmed. In addition, brief taxonomic notes on Bombus (Thoracobombus ) brevivillus Franklin (1913) and Bombus (Thoracobombus ) morio Swederus (1787), the sympatric species, are provided. A key for identification of Brazilian Bombus species, including the new species, was elaborated.Bumblebee / Bombus sp. nov. / DNA barcoding / nuclear markers / traditional taxonomy

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Male labial gland secretions and mitochondrial DNA markers support species status of Bombus cryptarum and B. magnus (Hymenoptera, Apidae)

Cited by 69 publications

References 14 publications

Revealing the hidden niches of cryptic bumblebees in Great Britain: Implications for conservation

Revealing the hidden niches of cryptic bumblebees in Great Britain: Implications for conservation

Notes on distribution of Bombus cryptarum (Hymenoptera, Apoidea) in Moravian territory (Czech Republic) and its laboratory rearing

An integrative approach identifies a new species of bumblebee (Hymenoptera: Apidae: Bombini) from northeastern Brazil

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