Male Pheromones Induce Ovulation in Female Honeycomb Groupers (Epinephelus merra): A Comprehensive Study of Spawning Aggregation Behavior and Ovarian Development

Abstract

This study characterizes the spawning phenomena of the honeycomb grouper (Epinephelus merra), which is a lunar-synchronized spawner that spawns a few days after full moon. To elucidate the aggregation characteristics of wild honeycomb groupers, the numbers of males and females at the spawning grounds were counted before and after the full moon. Approximately 20 males were consistently observed at the spawning grounds throughout the study period. Females appeared several days after full moon and rapidly increased in number, peaking four days after full moon (41 individuals). The maturation status of the females aggregating at the spawning grounds was investigated. The gonadosomatic index increased rapidly three days after full moon, and ovulation was confirmed. Individuals with ovulatory eggs were present for three days, after which the number of females at the spawning grounds decreased. Additionally, the role of males in final oocyte maturation (FOM) and ovulation in females during the spawning phase was investigated in captivity. FOM was induced in females reared in water with mature males, suggesting that male pheromones in the water induced FOM via activation of the hypothalamic–pituitary–gonadal axis. This suggests that spawning at the natural spawning grounds was the result of male–female interactions via pheromones.

Endocrine Regulation of Maturation and Sex Change in Groupers

“…To avoid visual cues, the structure of the rearing system was designed so the females in the lower aquarium could not visually recognize the males in the upper aquarium ( Figure 2 ). The results indicated that final maturation progressed and ovulation occurred in mature females exposed to the rearing water of mature males, but not that of the control tank [ 51 ]. These results demonstrate that the execution of FOM and ovulation in honeycomb grouper females requires pheromones from mature males.…”