Male reproduction is affected by RNA interference of period and timeless in the desert locust Schistocerca gregaria

Tobback, Julie; Boerjan, Bart; Vandersmissen, Hans Peter; Huybrechts, Roger

doi:10.1016/j.ibmb.2011.11.003

Cited by 20 publications

(11 citation statements)

References 48 publications

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“…Due to the inconsistent results of these studies, we suggest that this relationship could be affected by the circadian system, which has been established to be important in mature sperm production process. [27][28][29][30] Inappropriate sleep habits disrupt the rhythmic expression of circadian genes, leading to adverse effects on the male reproductive system. 31,32 Since the circadian sleep-wake cycle is regulated by melatonin, endogenous circadian rhythm disorders could disrupt the cycle by deregulating the production of melatonin and cortisol.…”

Section: Discussionmentioning

confidence: 99%

<p>Association Between Sleep Quality and Semen Parameters and Reproductive Hormones: A Cross-Sectional Study in Zhejiang, China</p>

Du¹,

Yang²,

Chen³

et al. 2020

NSS

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Background: The effects of sleep duration on semen quality have been documented in many epidemiological studies. However, the association between sleep quality and semen parameters and reproductive hormones is still unclear. Patients Enrollment and Methods: We conducted a cross-sectional study among 970 outpatients from the Reproductive Medicine Center in Zhejiang, China between October 2017 and July 2019. All participants delivered a semen sample, underwent a physical examination, and answered a questionnaire to provide the following information: demographics, life habits, and sleep habits. Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). We first divided the patients into two groups according to sleep quality (good sleep: PQSI < 5 and poor sleep: PSQI ≥ 5). Then, we analyzed routine sperm parameters (semen volume, sperm total motility, progressive motility, sperm concentration, total sperm number, and normal sperm morphology) and reproductive hormones (folliclestimulating hormone, luteinizing hormone, estrogen, testosterone, and prolactin) of each group. Finally, we used multivariate linear regression analysis and Spearman correlation coefficients to examine the relationship between sleep quality (discrete variable or dichotomous variable) and sperm parameters, reproductive hormones. Results: A negative correlation was found between the general PSQI scores and several semen parameters: total motility (r= −0.187979, p< 0.001), progressive motility (r= −0.192902, p< 0.001), concentration (r= −0.167063, p< 0.001), total sperm number (r= −0.160008, p< 0.001), and normal sperm morphology (r= −0.124511, p< 0.001). However, there was no significant correlation between the semen volume, all reproductive hormones and the general PSQI scores. After adjusting for confounders, men with poor sleep had lower total motility (β= −9.287; 95% CI, −12.050, −6.523), progressive motility (β= −8.853; 95% CI, −11.526, −6.180), concentration (log scale, β= −0.131; 95% CI, −0.181, −0.082), total sperm number (log scale, β= −0.137; 95% CI, −0.189, −0.084), and normal sperm morphology (β= −1.195; 95% CI, −1.844, −0.547), but semen volume and all reproductive hormones were not markedly altered. Conclusion: Poor sleep quality might be related to impaired semen quality, but we found no evidence that poor sleep quality affects reproductive hormones.

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Section: Discussionmentioning

confidence: 99%

<p>Association Between Sleep Quality and Semen Parameters and Reproductive Hormones: A Cross-Sectional Study in Zhejiang, China</p>

Du¹,

Yang²,

Chen³

et al. 2020

NSS

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“…Since S. gregaria shows a highly potent and systemic RNAi response, a knockdown of the Schgr-sNPFp transcript in this tissue will also be indicative of effects on other tissues. The high efficiency of RNAi in multiple tissues of the desert locust has previously been demonstrated in a number of studies and has been studied in detail by Wynant et al [17,27,28,34]. Total RNA was extracted from the dissected tissues and was reverse transcribed into cDNA and used as template in qRT-PCR analysis, as described previously for the Schgr-sNPF precursor transcript distribution.…”

Section: Forward Primer (5 -3 )mentioning

confidence: 99%

Identification of the short neuropeptide F precursor in the desert locust: Evidence for an inhibitory role of sNPF in the control of feeding

et al. 2014

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Peptides of the short neuropeptide F (sNPF) family have been shown to modulate feeding behavior in a wide variety of insect species. While these peptides stimulate feeding and food-searching behavior in Drosophila melanogaster and Apis mellifera, an opposite effect has recently been demonstrated in the desert locust, Schistocerca gregaria. In this study, we elaborate on these observations with the identification of the nucleotide sequence encoding the Schgr-sNPF precursor and the study of its role in the regulation of locust feeding behavior. We confirm that both Schgr-sNPF-like peptides, previously identified in mass spectrometric studies, are genuine precursor-encoded peptides. RNA interference mediated silencing of the Schgr-sNPF precursor transcript generates novel evidence for an inhibitory role of Schgr-sNPF in the regulation of feeding in S. gregaria. Furthermore, we show that starvation reduces the Schgr-sNPF precursor transcript level in the optic lobes, the primary visual centers of the locust brain. Our data indicate that Schgr-sNPF exerts an inhibitory effect on food uptake in the desert locust, which contrasts with effects of sNPF reported for several other insect species.

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“…This gene has been found to regulate the cell cycle (Unsal‐Kacmaz et al ., 2005, 2007; Gotter et al ., 2007). However, it should be noted that previous RNAi studies on tim in the desert locust did not yield a lethal phenotype (Tobback et al ., 2011, 2012) and mammalian tim is more similar to the Drosophila timeout gene than to the tim gene (Gotter, 2006) but one cannot exclude the possibility of a similar function for locust TIM based on what we know so far. Moreover, in both C. elegans and D. melanogaster , a knockout of the timeout gene is lethal as it interferes with normal development (Chan et al ., 2003; Benna et al ., 2010).…”

Section: Discussionmentioning

confidence: 90%

“…Applying an RNA interference (RNAi) approach that had already been proven to be very successful in the desert locust for studying its reproduction and development (Badisco et al ., 2011; Boerjan et al ., 2011, 2012; Marchal et al ., 2011a, b; Ott et al ., 2011), it was found that per and tim affect reproduction in both males and females of the desert locust, as is also the case in other insects. Intriguingly, a knock down of clk resulted in the premature death of the treated animals (Tobback et al ., 2011, 2012).…”

Section: Introductionmentioning

confidence: 99%

RNA interference mortality points to noncircadian functions for the clock gene in the desert locust Schistocerca gregaria

Tobback¹,

Vuerinckx²,

Boerjan³

et al. 2012

Insect Molecular Biology

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One of the core genes in the circadian regulation network is clock (clk). By forming a heterodimer with CYCLE (CYC) that binds on an E-box in the promoter region, it induces the transcription of other elements in the circadian transcriptional feedback loops and different clock output genes. In contrast to other insects, a clk double-stranded RNA (dsRNA) treatment is lethal in adults and fifth instar nymphs of the desert locust, Schistocerca gregaria, in a dose-dependent manner. Clk knock down fifth instar nymphs are able to undergo their imaginal moult but, depending on the amount of dsRNA, it takes them longer than the controls to reach adulthood. As adults, clk knock down animals do not develop their fat body and ovaries like the control animals. Therefore, we tested the expression of different genes involved in energy metabolism and reproduction to see the effect of the clk RNA interference knock down. Surprisingly, the expression of the vitellogenin gene was up-regulated in the clk knock down females who did not appear to invest their energy in egg development. Taken together, our results point out that the clk gene in the desert locust has an additional function in development besides its established role in maintaining the circadian rhythms in the brain.

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Male reproduction is affected by RNA interference of period and timeless in the desert locust Schistocerca gregaria

Cited by 20 publications

References 48 publications

<p>Association Between Sleep Quality and Semen Parameters and Reproductive Hormones: A Cross-Sectional Study in Zhejiang, China</p>

<p>Association Between Sleep Quality and Semen Parameters and Reproductive Hormones: A Cross-Sectional Study in Zhejiang, China</p>

Identification of the short neuropeptide F precursor in the desert locust: Evidence for an inhibitory role of sNPF in the control of feeding

RNA interference mortality points to noncircadian functions for the clock gene in the desert locust Schistocerca gregaria

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