Male reproductive development: gene expression profiling of maize anther and pollen ontogeny

Ma, Junjun; Skibbe, David S.; Fernandes, John; Walbot, Virginia

doi:10.1186/gb-2008-9-12-r181

Cited by 88 publications

(167 citation statements)

References 32 publications

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“…In the stage T library, the variety of genes was greatly decreased. The phenomenon of the decrease in number of expressed genes in the tetrad stage was also observed in other species (Ma et al 2008). This result might show that pollen development nursed by the tapetum was active in this stage.…”

Section: Discussionsupporting

confidence: 51%

“…In stage M, pollen-specific genes were isolated and the variety of isolated genes increased. In maize, the number of expressed genes increased after meiosis (Ma et al 2008). These authors considered the anther to be an integrated system, in which activation of anther maturation in the somatic cell layers is contingent on the successful completion of meiosis.…”

Section: Discussionmentioning

confidence: 99%

“…1), using the SSH method. Ma et al (2008) reported that approximately 80% of 15,950 transcripts shared across all anther stages are also expressed in leaves. Becker et al (2003) analyzed transcriptional profiling of Arabidopsis pollen.…”

Section: Discussionmentioning

confidence: 99%

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Spatiotemporal gene expression profiles associated with male strobilus development in Cryptomeria japonica by suppression subtractive hybridization

et al. 2011

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To identify the genes associated with the formation and development of the male strobilus of Cryptomeria japonica D. Don (Sugi), male strobilus-specific suppression subtractive hybridization (SSH) libraries were constructed from three different stages of male strobilus development. From these SSH libraries, 1,012 unigene sets including 314 contigs were assembled from 2,448 sequences. The profiles of the genes isolated from the SSH libraries differed strongly according to developmental stage: 49%, 13% and 29% of genes were unique to the early stage (stage E), tetrad stage (stage T), and mature stage (stage M) SSH libraries, respectively. To evaluate the reliability of the SSH libraries, we focused on the eight genes strongly concentrated in the three SSH libraries and performed RT-PCR analysis. All genes tested were expressed more strongly in the male strobilus than in the shoot. Our findings suggest that the gene profiles associated with male strobilus development differ in both number and kind of dominantly expressed genes.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

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Spatiotemporal gene expression profiles associated with male strobilus development in Cryptomeria japonica by suppression subtractive hybridization

et al. 2011

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“…Developmental transcriptomic profiling of anther development was previously reported in Brassica rapa (Dong et al, 2013), rice (Fujita et al, 2010), and maize (Zea mays; Ma et al, 2008), all of which employed microarrays. We applied the same statistical standard using one-way ANOVA analysis in MultiExperiment Viewer (MeV; P value , 0.001) and detected only three up-regulated genes in the F2 stage of B. rapa whole flower bud collection, 87 up-regulated genes in the Q stage of maize anther collection, and 458 up-regulated probes in M2 and M3 stage rice anthers (see "Materials and Methods").…”

Section: Anther Stage 9-specific Module Transiently Expresses a Largementioning

confidence: 99%

“…Transcriptomic studies have been conducted in pollen, sperm, and anthers in diverse plant species, including Arabidopsis, rice, B. rapa, maize, and cotton (Gossypium hirsutum; Honys and Twell, 2004;Ma et al, 2007Ma et al, , 2008Ma et al, , 2012Chen et al, 2010;Fujita et al, 2010;Deveshwar et al, 2011;Yang et al, 2011;Dong et al, 2013;Wuest et al, 2013). While some studies were focused on tapetum and male gametophytes, others compared anthers of male sterile mutants with anthers of the wild type.…”

Section: Anthers Of Floral Stage 9 Transiently Express a Large Numbermentioning

confidence: 99%

Floral Transcriptomes in Woodland Strawberry Uncover Developing Receptacle and Anther Gene Networks

et al. 2014

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ORCID IDs: 0000-0002-6902-740X (C.K.); 0000-0001-9969-9381 (Z.L.).Flowers are reproductive organs and precursors to fruits and seeds. While the basic tenets of the ABCE model of flower development are conserved in angiosperms, different flowering plants exhibit different and sometimes unique characteristics. A distinct feature of strawberry (Fragaria spp.) flowers is the development of several hundreds of individual apocarpous (unfused) carpels. These individual carpels are arranged in a spiral pattern on the subtending stem tip, the receptacle. Therefore, the receptacle is an integral part of the strawberry flower and is of significant agronomic importance, being the precursor to strawberry fruit. Taking advantage of next-generation sequencing and laser capture microdissection, we generated different tissue-and stage-transcriptomic profiling of woodland strawberry (Fragaria vesca) flower development. Using pairwise comparisons and weighted gene coexpression network analysis, we identified modules of coexpressed genes and hub genes of tissue-specific networks. Of particular importance is the discovery of a developing receptacle-specific module exhibiting similar molecular features to those of young floral meristems. The strawberry homologs of a number of meristem regulators, including LOST MERISTEM and WUSCHEL, are identified as hub genes operating in the developing receptacle network. Furthermore, almost 25% of the F-box genes in the genome are transiently induced in developing anthers at the meiosis stage, indicating active protein degradation. Together, this work provides important insights into the molecular networks underlying strawberry's unique reproductive developmental processes. This extensive floral transcriptome data set is publicly available and can be readily queried at the project Web site, serving as an important genomic resource for the plant biology research community.

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Comparative proteomic analysis of mitochondrial proteins from maize CMS‐C sterile, maintainer and restorer anthers

Zhang

Wang

et al. 2020

The Plant Genome

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The maize C system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rf4 have been widely used for maize hybrid production; however, the underlying mechanism is still uncertain. The sterility factor functions in mitochondria, where it interacts directly or indirectly with the restorer. Mitoproteomics can capture all participants involved in CMS and restoration at the organelle level. In the present study, we identified and quantified anther mitochondrial proteins from CMS, maintainer and restorer lines. We obtained 14,528 unique peptides belonging to 3,369 proteins. Comparative analysis of 1840 high-confidence proteins revealed 68 were differentially accumulated proteins likely involved in CMS or its restoration within mitochondria. These proteins were mainly associated with fatty acid metabolism, amino acid metabolism and protein-processing pathways. These results suggest that an energy deficiency caused by the sterility factor hinders other proteins or protein complexes required for pollen development through nuclear-mitochondrial interaction. The restorer factor may boost the energy generation by activating alternative metabolic pathways and by improving the post-translation processing efficiency of proteins in energy-producing complexes to restore pollen fertility. Our findings may aid detailed molecular analysis and contribute to a better understanding of maize CMS-C restoration and sterility. 1 INTRODUCTION Plant cytoplasmic male sterility (CMS), the inability to produce functional pollen, is a widespread plant reproductive feature (Chen & Liu, 2014). This maternally inherited trait has Abbreviations: CMS, cytoplasm male sterility; DAP, differentially accumulated protein; iTRAQ, isobaric tags for relative and absolute quantification. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Male reproductive development: gene expression profiling of maize anther and pollen ontogeny

Cited by 88 publications

References 32 publications

Spatiotemporal gene expression profiles associated with male strobilus development in Cryptomeria japonica by suppression subtractive hybridization

Spatiotemporal gene expression profiles associated with male strobilus development in Cryptomeria japonica by suppression subtractive hybridization

Floral Transcriptomes in Woodland Strawberry Uncover Developing Receptacle and Anther Gene Networks

Comparative proteomic analysis of mitochondrial proteins from maize CMS‐C sterile, maintainer and restorer anthers

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