Male sterility in onion (Allium cepa L.): origin: origin, evolutionary status, and their prospectus

Manjunathagowda, Dalasanuru Chandregowda; Muthukumar, P.; Gopal, J.; Prakash, M; Bommesh, J. C.; Nagesh, G. C.; Megharaj, Kaginalli Chandrashekarappa; Manjesh, G N; Anjanappa, M.

doi:10.1007/s10722-020-01077-1

Cited by 19 publications

(12 citation statements)

References 89 publications

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“…Numbers without superscripts indicate predicted PCR products with primers having 0-2 mismatches per primer on the target template. b Allium cepa male-sterilizing (KU318712.1) and normal (AP018390.1) cytoplasms representing monocot order Asparagales with the male sterilizing cytoplasm of inter-specific origin ( Manjunathagowda et al., 2021 ). c Cocos nucifera (KX028885.1) representing monocot order Arecacales.…”

Section: Resultsmentioning

confidence: 99%

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Plant mitochondrial introns as genetic markers - conservation and variation

et al. 2023

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Plant genomes are comprised of nuclear, plastid and mitochondrial components characterized by different patterns of inheritance and evolution. Genetic markers from the three genomes provide complementary tools for investigations of inheritance, genetic relationships and phenotypic contributions. Plant mitochondrial genomes are challenging for universal marker development because they are highly variable in terms of size, gene order and intergenic sequences and highly conserved with respect to protein-coding sequences. PCR amplification of introns with primers that anneal to conserved, flanking exons is effective for the development of polymorphic nuclear genome markers. The potential for plant mitochondrial intron polymorphisms to distinguish between congeneric species or intraspecific varieties has not been systematically investigated and is possibly constrained by requirements for intron secondary structure and interactions with co-evolved organelle intron splicing factors. To explore the potential for broadly applicable plant mitochondrial intron markers, PCR primer sets based upon conserved sequences flanking 11 introns common to seven angiosperm species were tested across a range of plant orders. PCR-amplified introns were screened for indel polymorphisms among a group of cross-compatible Citrus species and relatives; two Raphanus sativus mitotypes; representatives of the two Phaseolus vulgaris gene pools; and congeneric pairs of Cynodon, Cenchrus, Solanum, and Vaccinium species. All introns were successfully amplified from each plant entry. Length polymorphisms distinguishable by gel electrophoresis were common among genera but infrequent within genera. Sequencing of three introns amplified from 16 entries identified additional short indel polymorphisms and nucleotide substitutions that separated Citrus, Cynodon, Cenchrus and Vaccinium congeners, but failed to distinguish Solanum congeners or representatives of the Phaseolus vulgaris major gene pools. The ability of primer sets to amplify a wider range of plant species’ introns and the presence of intron polymorphisms that distinguish congeners was confirmed by in silico analysis. While mitochondrial intron variation is limited in comparison to nuclear introns, these exon-based primer sets provide robust tools for the amplification of mitochondrial introns across a wide range of plant species wherein useful polymorphisms can be identified.

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Section: Resultsmentioning

confidence: 99%

“… b Allium cepa male-sterilizing (KU318712.1) and normal (AP018390.1) cytoplasms representing monocot order Asparagales with the male sterilizing cytoplasm of inter-specific origin ( Manjunathagowda et al., 2021 ). …”

Section: Resultsmentioning

confidence: 99%

Plant mitochondrial introns as genetic markers - conservation and variation

et al. 2023

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“…The potential export value of Indian onion is approximately $460.52 [7]. Due to the constraints in traditional breeding of onion including signi cant heterozygosity, self-incompatibility, severe inbreeding depression, a biennial crop cycle and a restricted genetic base, the genetic improvement of onion has been limited [27,29,31]. The key prerequisites for the signi cant improvement of any crop are the availability of su cient genetic variability and rich genetic diversity [41].…”

Section: Introductionmentioning

confidence: 99%

“…Hence, molecular markers are considered to be more reliable in assessment of genetic variability than morphological data since they are not in uenced by environmental variables and are effective, rapid and able to test a large number of distinct genotypes at the DNA level [32]. Various molecular markers such as restriction fragment length polymorphism (RFLP) [9], random ampli ed polymorphic DNA (RAPD) [54][55], inter-simple sequence repeat (ISSR) [58], microsatellite or simple sequence repeat (SSR) [49], ampli ed fragment length polymorphism (AFLP) [53] and gene speci c markers [24,[27][28][29] have been employed for molecular analysis of onion genotypes.…”

Section: Introductionmentioning

confidence: 99%

Characterizing genetic diversity and population structure in onion (Allium cepa L.) genotypes using agro-morphological and molecular markers

Kapoor

Sood

Jayaswall

et al. 2023

Preprint

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Background It is crucial to gain understanding of crop diversity at the genetic and phenotypic levels. For breeding onion with higher yield and quality together with greater resilience to biotic and abiotic stressors agro-morphological and molecular characterization of onion genotypes is of utmost importance. Methods and results In the present study, genetic diversity in 49 onion genotypes were assessed using 6 agro-morphological descriptors, 19 quantitative traits along with 23 ISSR markers. All the agro-morphological descriptors were found polymorphic with Bulb: basic colour of dry skin (1.44) exhibiting the highest diversity index. The multivariate analysis using Mahalanobis D2 statistic grouped 49 genotypes into seven clusters with highest inter cluster distance between V and VII (364.35). A total of 78 fragments were produced from 13 polymorphic primers with a mean of 6 alleles per primer. The polymorphic information content (PIC) ranged from 0.42 (UBC 835) to 0.75 (UBC 825) with a mean of 0.61 per primer. With a mean of 0.36, the inter-genotype genetic distance ranged from 0.12 to 0.72. Based on cluster analysis using UPGMA algorithm, the genotypes were divided into two major clusters, whereas the cluster tree constructed using the described ISSR markers identified three major groups. The structure analysis divided the population into two main groups. Conclusion From the findings of present study, it can be stated that characterization at both molecular and morphological basis is of utmost importance to understand the genetic diversity in onion. Hybridization between distantly related genotypes can produce desirable transgressive segregants in future onion breeding programmes.

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“…The SEM was utilized to analyze the pollen morphology of species of the Acanthaceae family, allowing for microscale examination of pollen structure and surface variation (Al-Hakimi et al 2017). Analyses of male gametophytes utilizing light (Manjunathagowda and Anjanappa 2020; Manjunathagowda et al 2021) and SEM techniques have substantially aided the characterization of pollen structure, a further aid to the systematic understanding of plant breeding systems (Walker 1979). The topography of the tested sample and distributions of elements were captured using FESEM image analysis.…”

Section: Introductionmentioning

confidence: 99%

Characterization and relative mineral quantification of male gametophytes of garlic chives (Allium tuberosum Rottler ex Sprengel)

Manjunathagowda

Benke

Bhagat³

et al. 2021

Genet Resour Crop Evol

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Garlic chives (Allium tuberosum Rottler ex Sprengel) are an underutilized and neglected food crop of the Allium genus. For the future genetic development of alliums, phenotypic characterization and insights of breeding behaviour could be the aid to breed the new garlic chives varieties. Field Emission Scanning Electron Microscopy (FESEM) was used to reveal the morphological features of male gametophytes. The anther was reticulate, conspicuous, folded surface with scaly plates, and pollen was found to be sub-ovoid morphologies with long regulate and striated sexine. Spectra from FESEM-based Energy Dispersive X-ray Spectroscopy (EDS) revealed the elemental composition and distribution. The micrograph of an anther sample revealed the presence of O,

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Male sterility in onion (Allium cepa L.): origin: origin, evolutionary status, and their prospectus

Cited by 19 publications

References 89 publications

Plant mitochondrial introns as genetic markers - conservation and variation

Plant mitochondrial introns as genetic markers - conservation and variation

Characterizing genetic diversity and population structure in onion (Allium cepa L.) genotypes using agro-morphological and molecular markers

Characterization and relative mineral quantification of male gametophytes of garlic chives (Allium tuberosum Rottler ex Sprengel)

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