Male Sterility Systems in Major Field Crops and Their Potential Role in Crop Improvement

Saxena, K B; Hingane, Anupama

doi:10.1007/978-81-322-2286-6_25

Cited by 17 publications

(17 citation statements)

References 40 publications

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“…Male sterility (MS) is important in crop breeding because it greatly facilitates the production of hybrid seeds and utilization of heterosis on a large scale [ 3 ]. MS exists widely in flowering plants since male reproductive development can be disturbed in various processes, including stamen specification, anther cell division or differentiation, microsporocyte meiosis, tetrad microspore release, microspore mitosis, pollen wall development, and anther dehiscence [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Wang

et al. 2018

IJMS

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DNA methylation is an essential epigenetic modification that dynamically regulates gene expression during plant development. However, few studies have determined the DNA methylation profiles of male-sterile rapeseed. Here, we conducted a global comparison of DNA methylation patterns between the rapeseed genic male sterile line 7365A and its near-isogenic fertile line 7365B by whole-genome bisulfite sequencing (WGBS). Profiling of the genome-wide DNA methylation showed that the methylation level in floral buds was lower than that in leaves and roots. Besides, a total of 410 differentially methylated region-associated genes (DMGs) were identified in 7365A relative to 7365B. Traditional bisulfite sequencing polymerase chain reaction (PCR) was performed to validate the WGBS data. Eleven DMGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. In particular, Bnams4 was hypo-methylated in 7365A, and its expression was up-regulated, which might affect other DMGs and thus control the male sterility. This study provided genome-wide DNA methylation profiles of floral buds and important clues for revealing the molecular mechanism of genic male sterility in rapeseed.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Wang

et al. 2018

IJMS

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“…Male sterility is an important trait in crop breeding and for the conservation of natural genetic diversity in plants [10,33,34].…”

Section: Discussionmentioning

confidence: 99%

Male Sterility is linked to the Flavonoid Biosynthesis Pathways in Prunus mira

Zhang¹

2020

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The authors state that they adhere with COPE guidelines on publishing ethics as described elsewhere at https://publicationethics.org/. The authors also undertake that they are not associated with any other third party (governmental or non-governmental agencies) linking with any form of unethical issues connecting to this publication. The authors also declare that they are not withholding any information that is misleading to the publisher in regard to this article.

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“…Heterosis in CMS-based hybrid breeding system in pigeonpea, world's first and only pulse, has achieved increased grain yield (> 30%) and disease resistance as compared to the pure line varieties (Saxena et al 2010). Commercial success of the pigeonpea hybrid system using the only available A4 cytoplasm-based CMS, not only depends on a stable, non-reverting source of male sterility, but also on the identification of robust genotypes that reversibly suppresses the male sterility trait (Saxena and Hingane 2015). While the CMS trait is inherited maternally, very little is known about the underlying molecular mechanism to efficiently control pollination for hybrid seed production.…”

Section: Discussionmentioning

confidence: 99%

“…Seeds of an A4 cytoplasm (C. cajanifolius) containing CMS line (ICPA 2039), the corresponding maintainer line (ICPB 2039), restorer line (ICPR 2438) and a restored hybrid (ICPH 2438) from medium maturity group were procured from Pigeonpea Breeding Unit of the International Crops Research Institute for Semi-Arid Tropics (ICRISAT) in Hyderabad, India. Details of the cytoplasmic nuclear/genetic male sterility have been described earlier (Saxena and Hingane 2015). Plants were grown in pots containing autoclaved sand and soil (1:1) mixture and maintained in glasshouse under a 16 h light, 8 h dark photoperiod at 25/23 °C (light/ dark) with 70-80% relative humidity.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence

et al. 2018

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A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytoplasmic male sterility (CMS). We identified a novel ORF (orf147) from the A4 cytoplasm of C. cajanifolius that was created via rearrangements in the CMS line and co-transcribes with the known and unknown sequences. The bi/poly-cistronic transcripts cause gain-of-function variants in the mitochondrial genome of CMS pigeonpea lines having distinct processing mechanisms and transcription start sites. In presence of orf147, significant repression of Escherichia coli growth indicated its toxicity to the host cells and induced partial to complete male sterility in transgenic progenies of Arabidopsis thaliana and Nicotiana tabacum where phenotype co-segregated with the transgene. The male sterile plants showed aberrant floral development and reduced lignin content in the anthers. Gene expression studies in male sterile pigeonpea, Arabidopsis and tobacco plants confirmed down-regulation of several anther biogenesis genes and key genes involved in monolignol biosynthesis, indicative of regulation of retrograde signaling. Besides providing evidence for the involvement of orf147 in pigeonpea CMS, this study provides valuable insights into its function. Cytotoxicity and aberrant programmed cell death induced by orf147 could be important for mechanism underlying male sterility that offers opportunities for possible translation for these findings for exploiting hybrid vigor in other recalcitrant crops as well.

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Male Sterility Systems in Major Field Crops and Their Potential Role in Crop Improvement

Cited by 17 publications

References 40 publications

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Male Sterility is linked to the Flavonoid Biosynthesis Pathways in Prunus mira

A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence

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