Maleimide-acetylcholine headed bolaamphiphilic vesicles made from ricinoleic acid: Prospective active targeted drug delivery systems

Ewonkem, Monique B.; Grinberg, Sarina

doi:10.1016/j.chemphyslip.2018.01.009

Cited by 1 publication

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…synthesized bolaamphiphiles vesicles, which both ends of which could be covalently conjugated with ACh and maleimide. [ 36 ] Compared with these two methods, our SiO 2 @PAA‐MT/ACh nanospheres were more facile to load ACh or MT cargos on them. Finally, we characterized the successful conjugation of Cy3‐modified aptamers on the SiO 2 @PAA‐MT/ACh nanospheres by observing the fluorescent signals at 566 nm.…”

Section: Resultsmentioning

confidence: 99%

A “Nano‐Courier” for Precise Delivery of Acetylcholine and Melatonin by C5a‐Targeted Aptamers Effectively Attenuates Reperfusion Injury of Ischemic Stroke

Xie

Gao

Liu

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

Overactive inflammatory response and excessive oxidative stress are the main pathophysiological culprits for cerebral ischemia/reperfusion injury (IRI) that arouse neuronal damage . The neurotransmitter acetylcholine (ACh) exerts anti‐inflammatory roles by stimulating α7 nicotinic acetylcholine receptor (α7nAChR) on microglia to activate the cholinergic anti‐inflammatory pathway. Simultaneously, as a circadian rhythm‐dependent hormone , melatonin (MT) possesses promising neuroprotective effects that eliminate reactive oxygen species (ROS) in the ischemic region. Relying on these, a biocompatible fluorescein isothiocyanate (FITC)‐labeled SiO2@PAA‐MT/ACh nanospheres are constructed to effectively alleviate oxidative stress and polarize microglial phenotype to suppress inflammatory response in cerebral IRI. Despite of biosafety and curative effects of ACh and MT, the poor aggregation in ischemic penumbra hinders their neuroprotection. To address that, complement component 5a (C5a) is used as a molecular target for delivery of ACh and MT. C5a conspicuously exists at local inflammatory sites of cerebral IRI, recruits immune cells, and mediates further release of inflammatory cytokines. Upon binding of anti‐C5a (aC5a) aptamers onto FITC‐labeled SiO2@PAA‐MT/ACh nanospheres, they can effectively target the ischemic penumbra and promote neurological recovery. Taken together, the current study suggests that the FITC‐labeled SiO2@PAA‐MT/ACh‐aC5a nanospheres after intravenous (i.v.) administration can act as an effective targeted nanotherapy to salvage neurons in cerebral IRI.

show abstract

Section: Resultsmentioning

confidence: 99%