Maleimide‐Functionalized Poly(2‐Oxazoline)s and Their Conjugation to Elastin‐Like Polypeptides

Nawroth, Jonas F.; McDaniel, Jonathan R.; Chilkoti, Ashutosh; Jordan, Rainer; Luxenhofer, Robert

doi:10.1002/mabi.201500376

Cited by 30 publications

(15 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, strain‐promoted azide–alkyne cycloaddition is an example of a conjugation tool that has been adapted to create PEG hydrogels and could equally be applied to PAOx with only minor modifications to the previously reported bicyclo[6.1.0]non‐4‐yne terminated PEtOx . Similarly, glyco–PAOx, POxylated–proteins, and PAOx–nucleic acids conjugates that were previously reported have potential to be modified for hydrogel synthesis using physical or covalent cross‐linking.…”

Section: Future Directionsmentioning

confidence: 99%

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Dargaville

Park

Hoogenboom

2018

Macromolecular Bioscience

View full text Add to dashboard Cite

The synthesis of poly(2-oxazoline)s has been known since the 1960s. In the last two decades, they have risen in popularity thanks to improvements in their synthesis and the realization of their potential in the biomedical field due to their "stealth" properties, stimuli responsiveness, and tailorable properties. Even though the bulk of the research to date has been on linear forms of the polymer, they are also of interest for creating network structures due to the relatively easy introduction of reactive functional groups during synthesis that can be cross-linked under a variety of conditions. This opinion article briefly reviews the history of poly(2-oxazoline)s and examines the in vivo data on soluble poly(2-oxazoline)s to date in an effort to predict how hydrogels may perform as implantable materials. This is followed by an overview of the most recent hydrogel synthesis methods and emerging applications, and is concluded with a section on the future directions predicted for these fascinating yet underutilized polymers.

show abstract

Section: Future Directionsmentioning

confidence: 99%

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Dargaville

Park

Hoogenboom

2018

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Another family of polymers which has a rich and versatile thermoresponsive profile and has received a lot of attention in recent years due to their applicability in various biomedical contexts are poly(2-oxazoline)s (POx) [3,14,15,16,17] These structural isomers of polypeptides are accessible via living cationic ring-opening polymerization (LCROP) of 5-membered cyclic imino ethers, the 2-oxazolines [18]. Besides the use of POx in, e.g., protein/drug conjugates [14,19,20,21,22,23,24,25], non-covalent drug delivery systems [26,27,28,29,30,31,32,33,34,35], and anti-fouling modifications/surfaces [36,37,38,39,40,41,42], a plethora of POx-based hydrogels as soft biomaterials have been developed [43,44,45,46]. The emergence of the latter was supported by the low cytotoxicity/high biocompatibility of POx-based hydrogels [43], enabling their application as, e.g., bioink [46].…”

Section: Introductionmentioning

confidence: 99%

Temperature-Dependent Rheological and Viscoelastic Investigation of a Poly(2-methyl-2-oxazoline)-b-poly(2-iso-butyl-2-oxazoline)-b-poly(2-methyl-2-oxazoline)-Based Thermogelling Hydrogel

Lübtow

Mrlík

Hahn

et al. 2019

JFB

View full text Add to dashboard Cite

The synthesis and characterization of an ABA triblock copolymer based on hydrophilic poly(2-methyl-2-oxazoline) (pMeOx) blocks A and a modestly hydrophobic poly(2-iso-butyl-2-oxazoline) (piBuOx) block B is described. Aqueous polymer solutions were prepared at different concentrations (1–20 wt %) and their thermogelling capability using visual observation was investigated at different temperatures ranging from 5 to 80 °C. As only a 20 wt % solution was found to undergo thermogelation, this concentration was investigated in more detail regarding its temperature-dependent viscoelastic profile utilizing various modes (strain or temperature sweep). The prepared hydrogels from this particular ABA triblock copolymer have interesting rheological and viscoelastic properties, such as reversible thermogelling and shear thinning, and may be used as bioink, which was supported by its very low cytotoxicity and initial printing experiments using the hydrogels. However, the soft character and low yield stress of the gels do not allow real 3D printing at this point.

show abstract

“…The assays realized with ELP-POx at different ELR-to-POx molar ratios showed a clear increase in PTX solubilization as the molar ratio of POx to ELR increased (1:2, 1:4 and 1:8). Indeed, these nanoparticles were able to solubilize the anti-cancer drug with up to 8 wt% loading [49].…”

Section: Nanoparticles From Hybrid Elrsmentioning

confidence: 99%

Genetically Engineered Elastin-based Biomaterials for Biomedical Applications

Santos

Serrano-Dúcar

González‐Valdivieso

et al. 2020

CMC

View full text Add to dashboard Cite

Protein-based polymers are some of the most promising candidates for a new generation of innovative biomaterials as recent advances in genetic-engineering and biotechnological techniques mean that protein-based biomaterials can be designed and constructed with a high degree of complexity and accuracy. Moreover, their sequences, which are derived from structural protein-based modules, can easily be modified to include bioactive motifs that improve their functions and material-host interactions, thereby satisfying fundamental biological requirements. The accuracy with which these advanced polypeptides can be produced, and their versatility, self-assembly behavior, stimuli-responsiveness and biocompatibility, means that they have attracted increasing attention for use in biomedical applications such as cell culture, tissue engineering, protein purification, surface engineering and controlled drug delivery. The biopolymers discussed in this review are elastin-derived protein-based polymers which are biologically inspired and biomimetic materials. This review will also focus on the design, synthesis and characterization of these genetically encoded polymers and their potential utility for controlled drug and gene delivery, as well as in tissue engineering and regenerative medicine.

show abstract

Maleimide‐Functionalized Poly(2‐Oxazoline)s and Their Conjugation to Elastin‐Like Polypeptides

Cited by 30 publications

References 52 publications

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Poly(2‐oxazoline) Hydrogels: State‐of‐the‐Art and Emerging Applications

Temperature-Dependent Rheological and Viscoelastic Investigation of a Poly(2-methyl-2-oxazoline)-b-poly(2-iso-butyl-2-oxazoline)-b-poly(2-methyl-2-oxazoline)-Based Thermogelling Hydrogel

Genetically Engineered Elastin-based Biomaterials for Biomedical Applications

Contact Info

Product

Resources

About