Maleness, its inheritance and control in the aquatic phycomycete Allomyces macrogynus

Olson, Lauritz W.; Nielsen, Tom A. B.; Heldt-Hansen, Hans Peter; Grant, Neil G.

doi:10.1016/s0007-1536(82)80017-x

Cited by 13 publications

(5 citation statements)

References 16 publications

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“…A. macrogynus all-male and all-female mutants have been isolated through a variety of mutagenesis techniques. Non-Mendelian mutants producing nearly 100% male gametangia have been isolated by means of acridine dye treatment, resulting in mitochondrial DNA damage (218,224,256). No all-female A. macrogynus non-Mendelian mutants have been isolated, although both all-male and all-female strains have been generated through mutagenesis of the nuclear genome (218).…”

Section: Allomyces Macrogynus: a Model Homothallic Fungusmentioning

confidence: 99%

The Evolution of Sex: a Perspective from the Fungal Kingdom

Lee

et al. 2010

Microbiol Mol Biol Rev

323

344

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SUMMARY Sex is shrouded in mystery. Not only does it preferentially occur in the dark for both fungi and many animals, but evolutionary biologists continue to debate its benefits given costs in light of its pervasive nature. Experimental studies of the benefits and costs of sexual reproduction with fungi as model systems have begun to provide evidence that the balance between sexual and asexual reproduction shifts in response to selective pressures. Given their unique evolutionary history as opisthokonts, along with metazoans, fungi serve as exceptional models for the evolution of sex and sex-determining regions of the genome (the mating type locus) and for transitions that commonly occur between outcrossing/self-sterile and inbreeding/self-fertile modes of reproduction. We review here the state of the understanding of sex and its evolution in the fungal kingdom and also areas where the field has contributed and will continue to contribute to illuminating general principles and paradigms of sexual reproduction.

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Section: Allomyces Macrogynus: a Model Homothallic Fungusmentioning

confidence: 99%

The Evolution of Sex: a Perspective from the Fungal Kingdom

Lee

et al. 2010

Microbiol Mol Biol Rev

323

344

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“…The germination of P. sedebokerense resting cysts is comparable to Physoderma species, where the central vacuole melts before the protrusion of the inner content (endosporangium) through the cell wall [ 23 , 26 ], and our observations suggest that the neutral storage lipids are consumed and polar membrane lipids are produced (change of the Nile Red fluorescence color from yellow to red, Figure 3 ). Morphologically, the resting cysts of Physoderma species have one side flattened where a lid detach (operculum) during germination, allowing the endosporangium to protrude [ 23 , 27 ], while the resting cysts of P. sedebokerense are spherical and the protrusion of the endo-sporangium was observed after breakage ( Figure 3 ), after that stage we observed propagules (amoeboid swarmers) indicating the release.…”

Section: Discussionmentioning

confidence: 61%

Drought Resistant Resting Cysts of Paraphysoderma sedebokerense Preserves the Species Viability and its Virulence

Alors,

Boussiba,

Zarka

2023

Plants

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The blastocladialean fungus P. sedebokerense is a facultative parasite of economically important microalgae and for this reason it has gained a lot of interest. P. sedebokerense has a complex life cycle which includes vegetative and resting stages. The resting cysts were assumed to play an essential role in survival by resisting drought, but this ability was never tested and the factors that trigger their formation were not evaluated. This study was aimed to induce resting cyst formation and germination in P. sedebokerense. At first, we tested the survival of P. sedebokerense liquid cultures and found that infectivity is retained for less than two months when the cultures were stored on the bench at room temperature. We noticed that dry cultures retained the infectivity for a longer time. We, thus, developed a method, which is based on dehydration and rehydration of the biomass, to produce, maintain, and germinate resting cysts of P. sedebokerense in both saprophytic and parasitic modes of growth. When the dry cultures were rehydrated and incubated at 30 °C, resting cysts asynchronously germinated after 5 h and the “endosporangium” was protruding outside of the cyst. Our method can be used to preserve P. sedebokerense for research purposes with the advantage of no need for expensive equipment.

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“…The gametophyte is hermaphroditic and there appears to be a relationship between respiration and sexual expression (24)(25)(26). We have found (15) that by adding antimycin, an inhibitor of the Cyt-mediated respiration to the culture medium for wild type A. macrogynus, the gametophyte which develops is predominantly male. In addition, acridine dye-induced mutants of A. macrogynus have been isolated which are predominantly male, and these mutants segregate in a nonMendelian fashion when crossed to wild type, and have low rates of respiration.…”

mentioning

confidence: 92%

Respiration of Gametangia of the Aquatic Phycomycete Allomyces macrogynus

Heldt-Hansen¹,

Grant²,

Olson³

1983

Plant Physiol.

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Gametagia of the aquatic phycomycete Allomyces macrogyus have a cyanide-and antimycin A-insensitive respiration, which is sensitive to salicylhydroxamic acid (alternative respiration). Propyl gallate is also an inhibitor of this alternative pathway, and propyl gallate is more efficient than hydroxamic acid. Gametangial respiration is insensitive to propyl gallate, but propyl gallate sensitivity is gradually established when the gametangia are titrated with cyanide. Carbonyl cyanide m-chlorophenyl hydrazone stimulates the cyanide-sensitive respiration and engages the alternative sensitive respiration. Sodium azide inhibits both the alternative and the cyanide-sensitive respiration, but the cyanide-sensitive respiration is inhibited 10 times more efficiently than the alternative respiration. Rotenone inhibits the total respiration and the propyl gallateinsensitive respiration by 33% and the cyanide-insensitive respiration by 43%.The (6) (Chytridiomycetes). This subgenus is characterized by having alternating, isomorphic, mycelial generations (6), which can be cultured separately. The gametophyte is hermaphroditic and there appears to be a relationship between respiration and sexual expression (24-26). We have found (15) that by adding antimycin, an inhibitor of the Cyt-mediated respiration to the culture medium for wild type A. macrogynus, the gametophyte which develops is predominantly male. In addition, acridine dye-induced mutants of A. macrogynus have been isolated which are predominantly male, and these mutants segregate in a nonMendelian fashion when crossed to wild type, and have low rates of respiration. This has led us to an investigation of gametangial

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Maleness, its inheritance and control in the aquatic phycomycete Allomyces macrogynus

Cited by 13 publications

References 16 publications

The Evolution of Sex: a Perspective from the Fungal Kingdom

The Evolution of Sex: a Perspective from the Fungal Kingdom

Drought Resistant Resting Cysts of Paraphysoderma sedebokerense Preserves the Species Viability and its Virulence

Respiration of Gametangia of the Aquatic Phycomycete Allomyces macrogynus

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