Malignancy, mortality, and medicosurgical management of Clostridium septicum infection

Larson, Christopher M.; Bubrick, Melvin P.; Jacobs, Donald M.; West, Michael A.

doi:10.1016/s0039-6060(05)80023-6

Cited by 123 publications

(85 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mutant cells retain some sensitivity to aerolysin because this Aeromonas toxin contains the carbohydrate-binding small lobe that mediates binding to membrane proteins, such as glycophorin, that are not GPI-anchored (39). 2 …”

Section: Gpi-deficient Cells That Are Completely Insensitive To Alphamentioning

confidence: 99%

“…Distal myonecrosis due to C. septicum occurs mainly in individuals with certain predisposing conditions such as colon cancer, leukemia, neutropenia, and diabetes (2). Although this pathogen secretes a number of toxic proteins, including deoxyribonuclease and hyaluronidase, the lethal, cytolytic alpha toxin is implicated as the major virulence factor and appears to be the immunodominant extracellular antigen (3).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Clostridium septicum Alpha Toxin Uses Glycosylphosphatidylinositol-anchored Protein Receptors

Gordon¹,

Nelson²,

Buckley³

et al. 1999

Journal of Biological Chemistry

139

111

View full text Add to dashboard Cite

The alpha toxin produced by Clostridium septicum is a channel-forming protein that is an important contributor to the virulence of the organism. Chinese hamster ovary (CHO) cells are sensitive to low concentrations of the toxin, indicating that they contain toxin receptors. Using retroviral mutagenesis, a mutant CHO line (BAG15) was generated that is resistant to alpha toxin. FACS analysis showed that the mutant cells have lost the ability to bind the toxin, indicating that they lack an alpha toxin receptor. The mutant cells are also resistant to aerolysin, a channel-forming protein secreted by Aeromonas spp., which is structurally and functionally related to alpha toxin and which is known to bind to glycosylphosphatidylinositol (GPI)-anchored proteins, such as Thy-1. We obtained evidence that the BAG15 cells lack N-acetylglucosaminyl-phosphatidylinositol deacetylase-L, needed for the second step in GPI anchor biosynthesis. Several lymphocyte cell lines lacking GPIanchored proteins were also shown to be less sensitive to alpha toxin. On the other hand, the sensitivity of CHO cells to alpha toxin was increased when the cells were transfected with the GPI-anchored folate receptor. We conclude that alpha toxin, like aerolysin, binds to GPIanchored protein receptors. Evidence is also presented that the two toxins bind to different subsets of GPIanchored proteins.

show abstract

Section: Gpi-deficient Cells That Are Completely Insensitive To Alphamentioning

confidence: 99%

mentioning

confidence: 99%

Clostridium septicum Alpha Toxin Uses Glycosylphosphatidylinositol-anchored Protein Receptors

Gordon¹,

Nelson²,

Buckley³

et al. 1999

Journal of Biological Chemistry

139

111

View full text Add to dashboard Cite

show abstract

“…Even with eff ective treatment, including debridement and antibiotics, the mortality rate approaches 60% (10). Th e drug of choice for this infection is penicillin G. Th e extended-spectrum cephalosporins, carbapenems, and metronidazole are the usual alternatives in patients allergic to penicillins.…”

Section: Discussionmentioning

confidence: 99%

FatalClostridium SepticumInfection in a Patient with a Hematological Malignancy

Panikkath¹,

Konala²,

Panikkath³

et al. 2014

Baylor University Medical Center Proceedings

View full text Add to dashboard Cite

“…septicum is a gram positive, spore forming, motile, anaerobic rod that can be found in normal human colonic flora [2]. It forms a number of exotoxins including a-toxin which produces hemolysis and is particularly potent at causing tissue necrosis [3]. Clostridium spores have a predilection to localize and germinate in necrotic tissue [4].…”

Section: Discussionmentioning

confidence: 99%

Clostridium septicum Infection Associated with Necrotizing Pancreatitis, Transmural Gastric Necrosis, and Liquefaction of the Spleen

Zhou

Martindale

2007

Eur J Trauma Emerg Surg

View full text Add to dashboard Cite

Spontaneous infection with Clostridium septicum (C. septicum) is rare, but when isolated it is commonly associated with malignancy. We report a case of a 47-year-old man with infected pancreatic necrosis involving greater than 80% of the pancreas, transmural gastric necrosis, and liquefaction necrosis of the spleen. Initial cultures revealed C. septicum infection. He was managed with prompt aggressive surgical debridément, resection, appropriate broad spectrum antibiotics, and early enteral nutrition.

show abstract

Malignancy, mortality, and medicosurgical management of Clostridium septicum infection

Cited by 123 publications

References 10 publications

Clostridium septicum Alpha Toxin Uses Glycosylphosphatidylinositol-anchored Protein Receptors

Clostridium septicum Alpha Toxin Uses Glycosylphosphatidylinositol-anchored Protein Receptors

FatalClostridium SepticumInfection in a Patient with a Hematological Malignancy

Clostridium septicum Infection Associated with Necrotizing Pancreatitis, Transmural Gastric Necrosis, and Liquefaction of the Spleen

Contact Info

Product

Resources

About