Malignant characteristics of circulating tumor cells and corresponding primary tumor in a patient with esophageal squamous cell carcinoma before and after surgery

Ren, Chuanli; He, Ping; Zhang, Jinqiang; Zheng, Zhaoxu; Qiao, Yuanyuan; Zhao, Xiaohang

doi:10.4161/cbt.11.7.14950

Cited by 15 publications

(16 citation statements)

References 25 publications

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“…The collected blood samples were transferred to 50 mL centrifuge tubes (Corning Inc., Corning, NY, USA), and buffer 1 (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO4, 2 mM ethylenediaminetetraacetic acid (EDTA), 0.5% BSA, pH 7.4) was added to a total volume of 50 mL 15–20. The tubes were centrifuged at 450× g for 5 minutes, and the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

“…Double immunofluorescence staining was performed with 100 μL of CK8/18/19-anti-cytokeratin-fluorescein isothiocyanate (anti-cytokeratin-FITC) (1:100, green) and anti-CD45-phycoerythrin (1:1,000, red; Miltenyi Biotec) for indirect immunofluorescence staining. The cells were blotted dry and mounted with medium containing the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich Corp.) 15,16,18. A blind review of the 3-color fluorescent images by 3 technicians confirmed CTC identity.…”

Section: Methodsmentioning

confidence: 99%

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Prognostic value of circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma

Qiao¹,

Shi³

et al. 2017

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ObjectiveCirculating tumor cells (CTCs) of patients with malignant tumors can be used as a prognostic marker. However, there are few relevant reports to date on esophageal squamous cell carcinoma (ESCC). Our study assesses the clinical significance of CTCs in ESCC patients.Patients and methodsCTCs were detected in 103 peripheral blood (PB) samples from 59 ESCC patients. Correlation between CTCs and clinical parameters was analyzed using the χ2 test or Fisher’s exact test. Overall survival (OS) and progression-free survival (PFS) were analyzed using Kaplan–Meier analysis and univariate and multivariate methods.ResultsThe CTC detection rate was 79.7% (47/59) at baseline. The frequency of CTC-positive patients increased as the disease stage advanced (88.0% in stages III–IV, 58.9% in stages I–II). CTC counts ≥0/7.5 mL of PB were correlated with the degree of tumor differentiation, tumor infiltration, and lymph node and distant metastases. Overall, the OS and PFS of patients with CTC counts ≥3 or ≥5/7.5 mL of PB before surgery were significantly shorter than those of patients with CTC counts <3 or <5/7.5 mL. Multivariate analysis showed CTC counts ≥5/7.5 mL of PB to be a strong prognostic indicator of OS (hazard ratio [HR] 12.478; 95% confidence interval [CI], 8.2–34.3; P<0.05) and PFS (HR 6.524; 95% CI, 1.2–34.3; P<0.05) in ESCC patients. Patients in whom CTCs changed from positive at baseline to a negative value after surgery had an excellent prognosis.ConclusionCTCs might serve as a reference indicator for the prognosis and monitoring of disease progression and treatment effects in ESCC.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Prognostic value of circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma

Qiao¹,

Shi³

et al. 2017

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“…We employed a method using CD45-coated immunomagnetic beads to enrich cancer cells from pleural blood (4,8). A total of 30 cancer cells/ml pleural effusion were enriched and detected (Table I).…”

Section: Discussionmentioning

confidence: 99%

Increased plasma microRNA and CD133/CK18-positive cancer cells in the pleural fluid of a pancreatic cancer patient with liver and pleural metastases and correlation with chemoresistance

et al. 2012

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Abstract. We report a case of notably increased plasma levels of microRNA (miR)-21, miR-25, miR-103 and miR-151 in a pancreatic cancer patient with liver and pleural metastases. CD45-coated immunomagnetic beads detected an enrichment of malignant cancer cells in the pleural fluid, and CD133 + CK18 + cancer cells were identified. Using computer tomography (CT) combined with cancer cells stained in the pleural fluid, a previously healthy 60-year-old male was diagnosed with pancreatic cancer with multiple liver tumor metastases. Cancer antigen 19-9 (CA19-9), alkaline phosphatase (ALP) and γ-glutamate-transpeptidase (γ-GT) were notably increased in the serum, and carcinoembryonic antigen (CEA) was increased in the pleural fluid. The patient succumbed to the disease three months following standard chemotherapy. The increased levels of plasma miR-21, miR-25, miR-103 and miR-151, as well as the identification of CD133 + CK18 + cells in the pleural fluid of a pancreatic cancer patient with liver metastases, may regulate the molecular mechanisms involved in chemoresistance. The patient was insensitive to chemotherapy and succumbed 3 months later. Full elucidation of the molecular and pathological features of pancreatic cancer may be a novel strategy for diagnosis and tailored therapy.

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“…It has been shown in esophageal cancer that the malignant characteristics of CTCs and their corresponding primary tumor during the disease process may predict tumor burden and the risk of relapse and metastasis. 29 In breast cancer and prostate cancer, apoptotic CTCs were frequently found in peripheral blood of these patients. 13,15 This can be used to provide relevant information to evaluate the response to therapy in cancer patients.…”

confidence: 99%

Detection of apoptotic circulating tumor cells in advanced pancreatic cancer following 5-fluorouracil chemotherapy

Ren

Han

Zhang³

et al. 2011

Cancer Biology & Therapy

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Malignant characteristics of circulating tumor cells and corresponding primary tumor in a patient with esophageal squamous cell carcinoma before and after surgery

Cited by 15 publications

References 25 publications

Prognostic value of circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma

Prognostic value of circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma

Increased plasma microRNA and CD133/CK18-positive cancer cells in the pleural fluid of a pancreatic cancer patient with liver and pleural metastases and correlation with chemoresistance

Detection of apoptotic circulating tumor cells in advanced pancreatic cancer following 5-fluorouracil chemotherapy

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