Malignant Neoplasm

Maeda, Yoshinobu; Kawahara, Akihiko; Nishikawa, Takeshi; Norimatsu, Yoshiaki

doi:10.1007/978-981-16-5011-6_11

Cited by 2 publications

(4 citation statements)

References 44 publications

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“…As findings to imply infiltration to the stroma, the malignant glands show a closely packed glandular pattern, back-to-back glands, with scanty stroma, and a complex papillary growth pattern with blood vessel, and these histologic changes are also reflected in cellular samples. [ 8 13 ] Thus, corresponding to a histologic closely packed glandular pattern in G1-EEC cases, AB staining showed reactivity with CS distributed in stromal regions[ 5 ] between the glands appearing as finely branched. [ 4 ] In cases where papillary cell clusters appeared, the tubular AB-positive material penetrating the cluster and branching inside the clusters of cancer cells was identified as a microtubular pattern.…”

Section: Discussionmentioning

confidence: 99%

Staining Pattern of Alcian Blue in Endometrial Cytology: Utility in Distinguishing Grade 1-Endometrial Endometrioid Carcinoma from Endometrial Glandular Stromal Breakdown

Hosokawa,

Yoshiaki,

Nishikawa

et al. 2024

Journal of Cytology

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Background and Objective: In endometrial cytology, differentiating endometrial glandular stromal breakdown (EGBD) from endometrial endometrioid carcinoma (G1-EEC) is often difficult. In this study, we provided a new focus on chondroitin sulfate (CS), a major substrate component of the endometrial stroma, and assessed the diagnostic utility of Alcian Blue (AB) staining in the differential diagnosis in liquid-based cytological (LBC) samples. Materials and Methods: LBC specimens from 19 patients with a proliferative endometrium, 36 with EGBD, and 30 with G1-EEC who underwent endometrial cytology were stained with AB (pH 1.0), and their reactivity was observed. In addition, immunocytochemical staining of CS and CD31 was performed for five cases each to evaluate their interrelationship with blood vessels. Results: Regarding the 30 G1-EEC cases, at least one of the three representative staining patterns was observed by AB staining: dot-like, microtubular, and finely branched linear patterns. Moreover, the inner portion of the tubular material observed by AB staining expressed CD31. Conversely, in the 36 EGBD cases, only five metaplastic clusters with irregular protrusions and condensed stromal clusters (CSCs) showed a dot-like positive pattern, and background CSCs did not show reactivity to AB staining in any of the cases. Furthermore, the vascular structure expressing CD31 in cell clusters was also unclear. Conclusions: We demonstrated that AB staining shows different staining patterns in G1-EEC and EGBD, reflecting their different tissue structures. Our data provide new insights into endometrial cell diagnosis changes and demonstrate that AB staining is a potential new diagnostic aid tool for the differentiation of G1-EEC from EGBD.

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Section: Discussionmentioning

confidence: 99%

Staining Pattern of Alcian Blue in Endometrial Cytology: Utility in Distinguishing Grade 1-Endometrial Endometrioid Carcinoma from Endometrial Glandular Stromal Breakdown

Hosokawa,

Yoshiaki,

Nishikawa

et al. 2024

Journal of Cytology

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“…However, these are not constant. 1,8,13,14,22 -Endometrial endometrioid carcinoma (EEC) EEC is the most common histological type of EC, accounting for more than 75% of all endometrial carcinomas. Early menarche, late menopause, nulliparity, obesity and diabetes are well-known risk factors for EEC.…”

Section: Endometrial Atypical Hyperplasia/mentioning

confidence: 99%

“…These tumours are referred to as 'endometrioid' due to their similarity to proliferative phase endometrium. 22,23 On histologic samples, G1-EEC shows the following features: (1) confluent glandular pattern, (2) an extensive papillary pattern and (3) cribriform bridging. Well-defined nuclear abnormalities and an obvious necrotic background are recognized, but these are not constant.…”

Section: Malignant Neoplasmmentioning

confidence: 99%

“…EEC is a malignant epithelial neoplasm displaying varying proportions of glandular, papillary and solid architecture, with neoplastic cells showing endometrioid differentiation. These tumours are referred to as ‘endometrioid’ due to their similarity to proliferative phase endometrium 22,23 …”

Section: Microarchitectural Features Of Cell Clusters In Lbc Reflecti...mentioning

confidence: 99%

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A review of the directly sampled endometrial cytology on LBC samples: Classification, microscopic criteria and beyond

Norimatsu,

Maeda,

Malara

et al. 2023

Cytopathology

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The Yokohama System for Reporting Endometrial Cytology (TYS) has been proposed by an expert meeting under the auspices of the International Academy of Cytology (IAC) in May 2016 at the IAC in Yokohama. Since its introduction, the TYS has been receiving worldwide acceptance, and this review aims to assess its global impact. The adoption of endometrial cytology as a diagnostic procedure has been hampered in the past by difficulties arising in interpreting the cellular findings due to a number of factors (such as excess blood, cellular overlapping and the complex physiology of endometrium). Recently, the use of liquid‐based cytology (LBC), with its ability to remove blood and mucus and to distribute cells uniformly in a thin layer on the slide, has provided an opportunity to re‐evaluate the role of endometrial cytology. LBC is a useful tool in the cytologic diagnosis and follow‐up of endometrial abnormalities, which remains complementary to the emerging molecular diagnostic cytopathology. The study of LBC from endometrial cytology could be challenging since it is affected by numerous look‐alikes and diagnostic pitfalls. This review discusses these various entities and takes into consideration the ancillary techniques that may be useful in the diagnostic procedure. In conclusion, our review of the published data suggests that the TYS is a valid classification scheme that has been widely accepted by cytopathologists globally, is highly reproducible and makes a valuable contribution to clinical therapeutic management. At present, molecular cytopathology is a rapidly evolving field of modern cytopathology, which underlines the effective interplay between genomics and cytology. This review aims to provide a comprehensive review of the drawbacks of endometrial cytopathology, particularly in terms of endometrial cancer diagnosis and molecular testing.

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Malignant Neoplasm

Cited by 2 publications

References 44 publications

Staining Pattern of Alcian Blue in Endometrial Cytology: Utility in Distinguishing Grade 1-Endometrial Endometrioid Carcinoma from Endometrial Glandular Stromal Breakdown

Staining Pattern of Alcian Blue in Endometrial Cytology: Utility in Distinguishing Grade 1-Endometrial Endometrioid Carcinoma from Endometrial Glandular Stromal Breakdown

A review of the directly sampled endometrial cytology on LBC samples: Classification, microscopic criteria and beyond

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