Malignant tumor cell lines produce interleukin-1-like factor

Miyauchi, Shunsuke; Moroyama, Takamasa; Kyoizumi, Seishi; Asakawa, Jun‐ichi; Okamoto, Tomoyoshi; Takada, Kazuaki

doi:10.1007/bf02623644

Cited by 36 publications

(10 citation statements)

References 24 publications

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“…Ability to manipulate the machinery of cell death is an obvious goal of medical research and effect on regulation of apoptosis might lead to new possibilities for cancer treatment [30]. It is well documented that the secondary metabolites of Sinularia sps have induced apoptotic DNA fragmentation and condensation of chromatin in A431 cells [31]. Keeping in view, investigations were conducted for the first time to study the action mechanism of MDTFC on human leukemia THP-1 cells.…”

Section: Discussionmentioning

confidence: 99%

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

et al. 2009

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Bioassay directed fractionation and purification led to the successful isolation of a furano sesquiterpene, Methyl 5-[(1E,5E)-2,6-Dimethyl octa-1,5,7-trienyl] furan-3-carboxylate (MDTFC), a bioactive component from a soft coral, Sinularia kavarittiensis. Its structure was determined by analyzing (1)H, (13)C NMR and FAB-MS. The results show that MDTFC could efficiently and selectively inhibit the proliferation of several human cancer cell lines. Among all the cell lines, THP-1 was found to be most sensitive (IC(50) 29.59 microM), whereas the peripheral blood mononuclear cells were least effected (IC(50) 464.16 microM). The molecular mechanism of MDTFC mediated apoptosis was investigated for the first time. Induction of apoptosis in THP-1 cells was characterized by cell membrane blebbing, chromatin condensation, DNA fragmentation, and decrease in level of pro-caspases 3, 9 and increase in Bax/Bcl-2 ratio. Our results were further strengthened through cleavage of poly (ADP-ribose) polymerase, reduction of mitochondrial membrane potential (Psim) and cytosolic release of cytochrome c, which are key events during apoptosis. Moreover, phosphatidyl serine exposure and appearance of sub-G1 peak also demonstrated cell death, when analyzed by flow cytometry. DNA fragmentation was prevented moderately when pretreated with caspase-9 inhibitor (Z-LEHD-FMK) and largely with caspase-3 inhibitor (Z-DEVD-FMK). In summary, MDTFC mediated apoptosis involves mitochondria-dependent pathway and the present compound of marine origin might have a therapeutic value against human cancer cell lines and especially on leukemia cells.

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Section: Discussionmentioning

confidence: 99%

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

et al. 2009

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“…The A431 human vulval-derived epidermoid carcinoma cell line 7) and Nakata oral squamous cell carcinoma cell line 8) were used in this study. These cell lines were maintained in a humidified atmosphere of 5% CO 2 /95% air at 37 C in an RD medium (1:1 mixture of an RPMI1640 medium and Dulbecco's modified Eagle's medium (DMEM; Sigma Co., St. Louis, MO, USA) supplemented with 5% calf serum (CS; Hyclone Laboratories, Logan, UT, USA).…”

Section: Methodsmentioning

confidence: 99%

“…To obtain information on the structure-activity relationship, we tested the cytotoxicity of this metabolite in comparison with that of the perhydro derivative, DHA, and other common fatty acids by using two human tumor cell lines, A431 and Nakata cells. 7,8) The results are summarized in Table 3; the IC 50 values for ZL, DHA, ZL-H 12 , linoleic acid, and linolenic acid were determined to be 0.23, 0.21, 7.1, 2.2, and 2.2 M, respectively, against A431 cells, and 0.27, 0.24, 3.5, 1.7, and 2.3 M, respectively, against Nakata cells. The low specific activity of ZL in comparison with that of the crude EtOAc extract would have been due to other cytotoxic metabolites in the EtOAc extract, one of which was confirmed to be the carotenoid, peridinin.…”

Section: Distribution Of Zl Within 11 Dinoflagellate Isolatesmentioning

confidence: 98%

Zooxanthellactone, a Novel γ-Lactone-type Oxylipine from Dinoflagellates ofSymbiodiniumsp.: Structure, Distribution, and Biological Activity

Onodera¹,

Fukatsu²,

Kawai³

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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A novel fatty acid derivative named zooxanthellactone (ZL) was isolated from several strains of symbiotic microalgae, dinoflagellates of the genus Symbiodinium. The metabolite is structurally related to docosahexaenoic acid (DHA) and seems to be biosynthesized by oxidation and subsequent lactonization. The absolute stereochemistry was determined from the specific rotation of the perhydro derivative. The distribution of ZL within several Symbiodinium isolates was quantitatively analyzed by HPLC techniques and suggested a relationship between the productivity of this metabolite and the Symbiodinium phylogeny. The cytotoxicity of ZL was evaluated by using human squamous cell carcinoma cell lines in comparison with that of DHA and other common fatty acids, suggesting that the long unsaturated chain was important rather than the gamma-lactone moiety.

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“…Tumour cells can also activate directly factor X by procoagulant cysteine proteinase, which has been found in some patients with lung, prostate, colon, breast and kidney cancers as well as in patients with leukaemia [34, 35]. Tumour cells can also activate the clotting system indirectly by stimulating mononuclear cells to synthesize and release various procoagulants [36, 37, 38]. …”

Section: Biological Plausibilitymentioning

confidence: 99%

Venous Thromboembolism as First Manifestation of Cancer

Piccioli

Prandoni

2001

Acta Haematol

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Since Trousseau’s time numerous studies have addressed the relationship between cancer and venous thromboembolism (VTE) providing firm evidence of the increased risk of subsequent clinically overt malignancy during the follow-up of patients with idiopathic VTE. These malignancies are not limited to certain subtypes, but involve virtually all body systems. This knowledge has led to a long-standing debate on the need to screen for occult malignancies patients with idiopathic VTE with no clinical evidence of cancer at the time of the index thrombotic event. In fact the high incidence of newly discovered cancers does not automatically imply that screening is indicated in these patients since it is unknown whether a substantial proportion of cancers can be diagnosed, whether the diagnosed cancers are treatable and what the impact on cancer-related mortality is. Proper clinical trials are being conducted to find an answer to these questions.

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Malignant tumor cell lines produce interleukin-1-like factor

Cited by 36 publications

References 24 publications

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

Furano-sesquiterpene from soft coral, Sinularia kavarittiensis: induces apoptosis via the mitochondrial-mediated caspase-dependent pathway in THP-1, leukemia cell line

Zooxanthellactone, a Novel γ-Lactone-type Oxylipine from Dinoflagellates ofSymbiodiniumsp.: Structure, Distribution, and Biological Activity

Venous Thromboembolism as First Manifestation of Cancer

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