Malondialdehyde as an Indicator of Oxidative Stress During Abdominal Aortic Aneurysm Repair

Papalambros, Efstathios; Sigala, Fragiska; Georgopoulos, Sotiris; Paraskevas, Kosmas I.; Andreadou, Ioanna; Menenakos, Xaralambos; Sigalas, Panagiotis; Papalambros, Alexandros; Vourliotakis, George; Γιαννόπουλος, Αθανάσιος; Bakoyiannis, Christos; Bastounis, Elias

doi:10.1177/0003319707305246

Cited by 36 publications

(32 citation statements)

References 44 publications

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“…21 Among them, blood levels of malondialdehyde were significantly increased in AAA patients. 22 Likewise, we have measured MPO in plasma of AAA patients, a wellrecognized oxidative stress biomarker of different cardiovascular pathologies, 23,24 showing that MPO plasma levels are increased not only in large AAA, 7 but also in small AAA. In the other hand, catalase plasma levels were significantly decreased in small and large AAA patients relative to control subjects.…”

Section: Discussionmentioning

confidence: 98%

Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm: Potential Implication of Oxidative Stress in Abdominal Aortic Aneurysm Progression

Ramos-Mozo

Madrigal‐Matute

Martinez-Pinna

et al. 2011

ATVB

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Objective-Polymorphonuclear neutrophils (PMNs) play a main role in abdominal aortic aneurysm (AAA) progression.We have analyzed circulating PMNs isolated from AAA patients and controls by a proteomic approach to identify proteins potentially involved in AAA pathogenesis. Methods and Results-PMNs from 8 AAA patients (4 large AAA Ͼ5 cm and 4 small AAA 3-5 cm) and 4 controls were analyzed by 2D differential in-gel electrophoresis. Among differentially expressed spots, several proteins involved in redox balance were identified by mass spectrometry (eg, cyclophilin, thioredoxin reductase, catalase). Diminished catalase expression and activity were observed in PMNs from AAA patients compared with controls. In contrast, PMNs from AAA patients displayed higher H 2 O 2 and myeloperoxidase levels than PMNs from controls. Moreover, a significant decrease in catalase mRNA levels was observed in PMNs after phorbol 12-myristate 13-acetate incubation. Catalase plasma levels were also decreased in large (nϭ47) and small (nϭ56) AAA patients compared with controls (nϭ34). We observed catalase expression in AAA thrombus and thrombus-conditioned medium, associated with PMN infiltration. Furthermore, increased H 2 O 2 levels were observed in AAA thrombus-conditioned medium compared with the media layer. Key Words: aneurysms Ⅲ antioxidants Ⅲ leukocytes A bdominal aortic aneurysm (AAA) is an important health problem in elderly. In cross-sectional studies, the prevalence varies from 3% to 8%. 1 In elderly men, AAAs may cause as much as 2% to 3% of all deaths. 1 Because AAAs are usually asymptomatic, the present clinical challenges are early diagnosis and deciphering the biological mechanisms responsible for the progressive dilatation and final rupture to develop new diagnostic and therapeutic approaches. Conclusion-DiminishedAlthough polymorphonuclear neutrophils (PMNs) represent the major class of leukocytes, they have received little attention in atherothrombosis. 2,3 However, recent evidence is revealing a previously unappreciated role of PMN in experimental 4,5 and human 6,7 AAAs. PMNs can contribute to main mechanisms of AAA evolution, namely intraluminal thrombus (ILT) formation, oxidative stress, proteolytic degradation of the aortic media, and adventitial inflammation. 8 AAAs are characterized by the presence of a mural ILT-containing platelets, red blood cells (RBCs), and PMNs, particularly abundant within the luminal layer of human thrombus. 6 -8 AAAs are also characterized by destructive connective tissue remodeling, including depletion of aortic elastin and fragmentation of medial elastic fibers. 9 Finally, inflammatory cells (macrophages and neutrophils) are also evident within the adventitia of human AAAs. 10 Interestingly, PMNs depletion is able to inhibit experimental AAA formation. 11 More recently, short-term preoperative doxycycline therapy improved the proteolytic balance in human AAA, presumably via an effect on aortic wall neutrophil content. 12 These data highlight the potential interest of analyzing the PMNs p...

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Section: Discussionmentioning

confidence: 98%

Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm: Potential Implication of Oxidative Stress in Abdominal Aortic Aneurysm Progression

Ramos-Mozo

Madrigal‐Matute

Martinez-Pinna

et al. 2011

ATVB

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“…In a previous study, lower TAS levels were found to be related to lower perioperative complications (11). Papalambros et al found MDA levels to be higher in patients with long lengths of stay in intensive care after elective or urgent AAA (2).…”

Section: Discussionmentioning

confidence: 99%

“…Being a less invasive procedure, less intense OS may contribute to EVAR's lower mortality and morbidity rates. IRI in OR not only results in local damage but also in distant organ injury (2,7,14). In AAA patients, it is reported that OS is correlated with myocardial infarction and lung damage (3,11,15,16).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of perioperative oxidative stress in endovascular and open repair of abdominal aortic aneurysm

Doğan¹,

Menteşe²,

Doğan³

et al. 2015

Turk J Med Sci

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Background/aim: To investigate the differences in perioperative oxidative stress (OS) in abdominal aortic aneurysm (AAA) patients treated with either endovascular repair (EVAR) or open repair (OR).Materials and methods: Twenty patients (11 OR, 9 EVAR) treated for AAA with no known malignant or inflammatory disease and an aneurysm diameter of over 5 cm and no rupture were included in the study. Blood samples were obtained preoperatively, during aortic occlusion, and 1 h and 24 h after reperfusion. Total antioxidant status (TAS), total oxidative stress (TOS), and malondialdehyde (MDA) levels were measured and oxidative stress index (OSI) was calculated.Results: OSI at 1 h and 24 h after reperfusion was higher in the EVAR group (P = 0.004 and P = 0.002, respectively). TAS levels were higher (P = 0.001, P = 0,029). MDA levels showed no difference (P = 0.291, P = 0.076). TOS levels were lower 24 h after reperfusion in the EVAR group (P = 0.018). Conclusion:Markers of oxidative stress were lower in the EVAR group. This may be associated with advantages of EVAR. More studies are required for a clear-cut conclusion.

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“…The main effect of I/R is the activation of inflammatory pathways with oxidative stress occurring during aortic clamping and subsequent reperfusion [7]. The synthesis of cytokines during the inflammatory processes generates an increase in concentrations of acute-phase proteins as a response to tissue destruction [8], while high-grade oxidative stress during AAA repair operation has been related with a complicated postoperative course and transfer to intensive care unit [9].…”

Section: Discussionmentioning

confidence: 99%

The effects of n-acetylcysteine and desferoxamine on IL-6, TNF-a, and oxLDL after infrarenal aortic clamping

Katseni¹,

Chalkias

Kaparos³

et al. 2015

Hellenic J Surg

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Aim-Background: Treatment of abdominal aortic aneurysms remains a challenging task. We examined the effects of n-acetylcysteine and desferoxamine on interleukin-6, tumour necrosis factor-a, and oxidized low density lipoprotein after infrarenal aortic clamping.Methods: Fifteen piglets were assigned to three groups. The control group (Group C) received saline as placebo (10-mL dilution, bolus), whereas the Group of n-acetylcysteine (Group NAC) received 150mg/kg IV n-acetylcysteine 30 min prior to aortic clamping and 50mg/kg/h after its release (reperfusion). The desferoxamine group (Group D) received IM 100mg/kg/d desferoxamine for 3 days prior to the experiment.Results: Interleukin-6 increased significantly one hour after aortic clamping, while administration of desferoxamine and n-acetylcysteine had no effect on its production. Tumour necrosis factor-a increased after aortic clamping and failed to normalise during the experiment in all groups. At 2 hours post-reperfusion, desferoxamine had a modest effect on tumour necrosis factor-a values, while in Group NAC, tumour necrosis factor-a had decreased by 50% at 10 min post-reperfusion. Oxidized low density lipoprotein in Group C did not increase significantly from baselines values. In Groups D and NAC, oxidized low density lipoprotein had increased at 10 min post-reperfusion, after which it progressively decreased until 2 hours post-reperfusion.Conclusions: Interleukin-6 and tumour necrosis factor-a increased significantly after aortic clamping. Administration of desferoxamine and n-acetylcysteine had no effect on interleukin-6, while administration of n-acetylcysteine reduced tumour necrosis factor-a by 50% at 10 min post-reperfusion. Although in Groups D and NAC oxidized low density lipoprotein increased post-reperfusion, it progressively decreased with time.

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Malondialdehyde as an Indicator of Oxidative Stress During Abdominal Aortic Aneurysm Repair

Cited by 36 publications

References 44 publications

Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm: Potential Implication of Oxidative Stress in Abdominal Aortic Aneurysm Progression

Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm: Potential Implication of Oxidative Stress in Abdominal Aortic Aneurysm Progression

Comparison of perioperative oxidative stress in endovascular and open repair of abdominal aortic aneurysm

The effects of n-acetylcysteine and desferoxamine on IL-6, TNF-a, and oxLDL after infrarenal aortic clamping

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