Maltohexaose-indocyanine green (MH-ICG) for near infrared imaging of endocarditis

Takemiya, Kiyoko; Røise, Joachim Justad; He, Maomao; Taing, Chung; Rodriguez, Alexander; Murthy, Niren; Goodman, Mark M.; Taylor, W. Robert

doi:10.1371/journal.pone.0247673

Cited by 5 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent reports on optical and PA imaging of infection have successfully utilized uorophore-conjugated antibiotics, [5][6][7][8] compounds metabolized by bacteria, [9][10][11] and monoclonal antibodies. 6,12 However, antimicrobial resistance may impede the localization of certain probes to the site of infection, and monoclonal antibodies are known to experience long circulation times and could have a limited ability to penetrate mature bio lms due to their size.…”

Section: Introductionmentioning

confidence: 99%

99m Tc-Labelled Photoacoustic Imaging Probe for Detection of Bacterial Infection

Swann,

Hernández-Valdés,

Silva

et al. 2024

Preprint

View full text Add to dashboard Cite

The development of a non-invasive infection-specific diagnostic probe holds the potential to vastly improve early-stage detection of infection, enabling precise therapeutic intervention and potentially reducing the incidence of antibiotic resistance. Towards this goal, a commercially available bacteria-targeting Zinc (II)-dipicolylamine (ZnDPA)-derived fluorophore, PSVue794, was assessed as a photoacoustic (PA) imaging probe (PIP). A radiolabeled version of the dye, [99mTc]Tc-PSVue794, was developed to facilitate quantitative biodistribution studies beyond optical imaging methods, which showed a target-to-non-target ratio of 10.1 ± 1.1, 12 hrs post-injection. The ability of the PIP to differentiate between bacterial infection, sterile inflammation, and healthy tissue in a mouse model, was then evaluated via PA imaging. The PA signal in sites of sterile inflammation (0.062 ± 0.012 a.u.) was not statistically different from that of the background (0.058 ± 0.006 a.u.). In contrast, high PA contrast was detected at sites of bacterial infection (0.176 ± 0.011 a.u.) as compared to background (0.081 ± 0.04 a.u., where P ≤ 0.03). This work demonstrates the potential of utilizing established fluorophores towards PAI and utilizing PAI as a modality in the distinction of bacterial infection from sites of sterile inflammation.

show abstract