Mammaglobin Is Found in Breast Tissue as a Complex with BU101

Colpitts, Tracey L.; Billing-Medel, Patricia; Friedman, Paula N.; Granados, Edward N.; Hayden, Mark A.; Hodges, Steve J.; Menhart, Nick; Roberts, Lisa; Russell, John A.; Stroupe, Stephen D.

doi:10.1021/bi010284f

Cited by 42 publications

(48 citation statements)

References 35 publications

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“…The Y-axis represents the copy number for the gene of interest per 1 ng of b-actin. Lane 1, metastatic breast tumor to ovary; 2 ± 19 metastatic breast tumors to lymph node; 20 ± 33 primary breast tumors; 34 normal breast expressed in breast tumors (Colpitts et al, 2001). Among the 62 clones we also found GATA-3 transcription factor, thrombospondin-1, and AP-2.…”

Section: Discussionmentioning

confidence: 82%

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

et al. 2002

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Identifying novel and known genes that are di erentially expressed in breast cancer has important implications in understanding the biology of breast tumorigenesis and developing new diagnostic and therapeutic agents. In this study we have combined two powerful technologies, PCRbased cDNA subtraction and cDNA microarray, as a high throughput methodology designed to identify cDNA clones that are breast tumor-and tissue-speci®c and are overexpressed in breast tumors. Approximately 2000 cDNA clones generated from the subtracted breast tumor library were arrayed on the microarray chips. The arrayed target cDNAs were then hybridized with 30 pairs of¯uorescent-labeled cDNA probes generated from breast tumors and normal tissues to determine the tissue distribution and tumor speci®city. cDNA clones showing overexpression in breast tumors by microarray were further analysed by DNA sequencing, GenBank and EST database searches, and quantitative real time PCR. We identi®ed several known genes, including mammaglobin, cytokeratin 19, ®bronectin, and hair-speci®c type II keratin, which have previously been shown to be overexpressed in breast tumors and may play an important role in the malignance of breast. We also discovered B726P which appears to be an isoform of NY-BR-1, a breast tissue-speci®c gene. Two additional clones discovered, B709P and GABA A receptor p subunit, were not previously described for their overexpression pro®le in breast tumors. Thus, combining PCRbased cDNA subtraction and cDNA microarray allowed for an e cient way to identify and validate genes with elevated mRNA expression levels in breast cancer that may potentially be involved in breast cancer progression. These di erentially expressed genes may be of potential utility as therapeutic and diagnostic targets for breast cancer.

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Section: Discussionmentioning

confidence: 82%

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

et al. 2002

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“…Mammaglobin is a 93-amino-acid protein with homology to other secretoglobineuteroglobin family members [4][5][6][7][8]. Mammaglobin forms a stable disulfide-linked dimer with lipophilin B [9,10]. Unlike other secretoglobin family members, mammaglobin mRNA expression is breast specific; roughly 80% of primary breast cancers express mammaglobin [11].…”

Section: Introductionmentioning

confidence: 99%

Detection of Mammaglobin in the Sera of Patients with Breast Cancer

Fanger¹,

Houghton²,

Retter³

et al. 2002

Tumor Biol

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Current procedures for the diagnosis of breast cancer are cumbersome and invasive, making detection of this disease difficult. A rapid screening test for early detection of breast cancer would allow for better management of this deadly disease. In this report, we show that, with the exception of the skin, mammaglobin mRNA is specifically expressed in mammary tissue and commonly overexpressed in breast cancer. Mammaglobin is not expressed in other types of cancer including colon, lung, ovarian, and prostate cancer. Breast-specific expression of mammaglobin protein was shown using immunohistochemical methods. Mammaglobin is secreted from both established breast cancer cell lines and primary breast carcinoma cells cultured in vitro. Using a monoclonal antibody-based assay for monitoring the presence of mammaglobin in serum, elevated levels of mammaglobin were detected in sera of patients with breast cancer, but not in healthy women. Thus, mammaglobin, which is overexpressed and secreted from breast carcinoma cells, is detectable in sera of patients with breast cancer and may provide a rapid screening test for the diagnosis and management of breast cancer.

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“…Moreover, the predicted protein is homologous to a family of proteins that includes rat steroid-binding protein (prostatein) subunit C3 (rPSC3), rabbit uteroglobin (rUg), The Clara cell 10-kDa protein, human mammaglobin B protein (also designated as lacryglobin or lipophilin C), and lipophilin B (also known as BU101) and cat major allergan. Uteroglobin family members form homodimers and heterodimers, and mammaglobin heterodimerizes with lipophilin B in an antiparallel manner that allows for formation of three disulfide bridges between the two molecules (7,8). These genes are localized and clustered at 11q13 and this family of proteins has recently been designated secretoglobins to emphasize their secreted nature and globin-like structural features (9).…”

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confidence: 99%

Identification of Mammaglobin as a Novel Serum Marker for Breast Cancer

Bernstein

Godbold

Raptis

et al. 2005

Clinical Cancer Research

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Purpose: Early detection of breast cancer has implications for the management and treatment of patients with this disease. Currently, there exist no highly sensitive and specific serologic biomarkers for detection of breast cancer. Mammaglobin is predicted to be a secreted protein, and expression of this gene seems to be highly specific in breast cancer. The present studies were undertaken to develop the mammaglobin protein as a serum biomarker for detection of breast cancer. Experimental Design: We characterized the mammaglobin protein as a secreted, 14-to 21-kDa species, which is likely post-translationally processed based on its predicted 7-kDa size. Immunostaining for mammaglobin was conducted. An ELISA was developed for the detection of the mammaglobin protein in serum, and levels were compared between women with and without breast cancer. A receiver operating characteristic curve was used to show sensitivity and specificity for cut points on the continuous mammaglobin scale. Results:The protein was detectable by immunostaining in 72% of breast tumors and not in other tumor types.The ELISA was highly sensitive and specific for detection of mammaglobin protein in tissue culture fluids of breast cancer cells and sera of breast cancer patients. The ELISA differentiated healthy women from those with breast cancer with accurate, repeatable results across time and under varying storage conditions. Conclusion: Our results indicate that mammaglobin, as measured by the ELISA, holds significant promise for breast cancer screening with the realistic potential to impact management of this disease.

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Mammaglobin Is Found in Breast Tissue as a Complex with BU101

Cited by 42 publications

References 35 publications

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays

Detection of Mammaglobin in the Sera of Patients with Breast Cancer

Identification of Mammaglobin as a Novel Serum Marker for Breast Cancer

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