Mammalian Cell Cultures on Micropatterned Surfaces of Weak-Acid, Polyelectrolyte Hyperbranched Thin Films on Gold

Amirpour, Mary Lee; Ghosh, Pradyut; Lackowski, William M.; Crooks, Richard M.; Pishko, Michael V.

doi:10.1021/ac000907f

Cited by 63 publications

(57 citation statements)

References 70 publications

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“…On the contrary, the influence of the polymer surface charges on cell adhesion and on the biological functions of attached cells has not been rigorously investigated. At the cellular scale, only a few studies are concerned with the direct evaluation of cellular interactions with synthetic polyelectrolytes, although they have already widely been used to render an artificial substrate either adhesive, as for poly(L-lysine), or nonadhesive, as for polyacrylyc acid coupled to poly (ethylene glycol) (Amirpour et al, 2001). Elbert et al (1999) used alginate polyelectrolyte films in order to build a nonadhesive barrier.…”

Section: Discussionmentioning

confidence: 99%

Cationic polyelectrolyte hydrogel fosters fibroblast spreading, proliferation, and extracellular matrix production: Implications for tissue engineering

Rosa

Cartenı̀

Petillo

et al. 2003

Journal Cellular Physiology

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Fibrous encapsulation is known to occur to many prosthetic implants and is thought to be due to the cells not adhering adequately to the surface. For developing new materials able to enhance cellular adhesion by mimicking extracellular matrix components, polyelectrolyte polymers, characterized by tunable surface charges, have been proposed. Here we demonstrate that panoply of cell functions over a two-dimensional substratum is influenced by surface charge. We have at first generated structurally related polyelectrolyte substrata varying in their positive surface charge amount and subsequently evaluated a variety of behaviors of human primary fibroblasts seeded on these polymers. The proportion of adherent, spreading, and proliferating cells was increased significantly on cationic hydrophilic surfaces when compared with the neutral base surface. The extent of cell spreading correlated with cytoskeleton organization as assessed using immunofluorescence techniques. In the key experiment, the presence of cationic charges on cell adhesion-resistant neutral surface increased the synthesis of collagen I and III, the release of their metabolites, and the expression of their mRNA by fibroblasts. Interestingly, the scarce collagen deposits on neutral polymer consisted, for the most part, of collagen I while collagen III was present only in traces probably due to the secretion of metalloproteinase-2 by non-adherent fibroblasts. Taken together, these results show that polyelectrolyte films may promote the attachment of fibroblast cells as well as their normal secretory phenotype. Both effects could be potentially useful in integrating soft connective tissue to the implant, decreasing the chance of its fibrous encapsulation.

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Section: Discussionmentioning

confidence: 99%

Cationic polyelectrolyte hydrogel fosters fibroblast spreading, proliferation, and extracellular matrix production: Implications for tissue engineering

Rosa

Cartenı̀

Petillo

et al. 2003

Journal Cellular Physiology

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“…normally maintained through a layer of cell adhesion proteins between substrate and cell (Shimizu et al 2003;Amirpour et al 2001), which allows the formation of specific (Haas and Plow 1994;Elbert and Hubbell 1996) and probably also unspecific (Elbert and Hubbell 1996;Lee et al 2006) interactions between cell membrane receptors (e. g. integrins, proteoglycans) and surface proteins. It might, however, be that on some surfaces cell attachment and spreading is also possible in the absence of serum and cell secreted proteins (Curtis and McMurray 1986;Curtis et al 1983).…”

Section: Attachment Of Anchorage Dependent Cells To a Surface Ismentioning

confidence: 99%

“…0 μg (a), 150 μg (b) and 300 μg (c) collagen per ml 3 wt.% alginate solution in PBS vital and proliferating. Below this threshold, apoptosis occurs (Kane et al 1999;Amirpour et al 2001;Neff et al 1999;Kooten et al 1992). …”

Section: Attachment Of Anchorage Dependent Cells To a Surface Ismentioning

confidence: 99%

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Johann

Baiotto²,

Renaud

2007

Biomed Microdevices

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In this paper the easy and reliable preparation of precise micropatterns on PDMS surfaces is described and the growth of HEK 293 cells on those patterns during culture over several days is examined. The first patterning approach described is based on soft-lithography and polyelectrolyte multilayer deposition. Two different soft-lithographic techniques are employed for creating surface patterns of PAH, PSS, untreated and oxidized PDMS. The growth behavior of HEK 293 cells is investigated on all the dual combinations of the four surfaces, and decreasing preference of the cells for the surfaces in the order PAH (-NH2)>ox-PDMS (-OH)>>PSS (-SO3-)>PDMS (-CH3) is revealed. As the second patterning approach a method is introduced, which allows the deposition of gel droplets in a microarray format utilizing differences in the surface wettability. This concept is new and expected to be very useful for various applications. Finally, a speculative explanation for the different cell spreading behavior is provided considering the interplay between individual cell-surface interactions and a permanent cell tractional force.

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“…From Amirpour et al [9] and Datsetan known, that the wettability and the surface structure on the materials surface influence the cell adhesion. chapter shows the results of the geometrical characteriz tion, measurements of the wetting behavior terization of cell reactions on the surface.…”

Section: Resultsmentioning

confidence: 99%

Laser induced surface structure on stainless steel influences cell viability

Vehse

Löbler

Schmitz

et al. 2012

Biomedical Engineering / Biomedizinische Technik

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The interaction of cells and tissue with implant surfaces significantly decides about the reaction of the organism in contact with an implant. The interface between implant and surrounding cell media influences the initiated immune response. An initial point to influence the cell-implant-interaction is to structure the surface of the implant. Laser techniques offer many degrees of freedom to generate micro structures on material surfaces. Using a frequency doubled Nd:YAG laser system, the surface of stainless steel (316L) samples was micro-structured. Micro structures in the range of 20-80 ȝm line width and 10-50 ȝm depth were chosen. Lattice structures, wave structures or nub structures were created on steel plates. L929 mouse fibroblasts were seeded on the plates in order to determine the cell viability and adhesion by confocal laser scanning microscopy. Non-toxic stainless steel (tested in aqueous media) shows significant distinctions in cell adhesion dependent from structure dimensions. Especially surface structures with intersecting square grids with a linewidth near 40 ȝm lead to good cell viability. Cells grown on a square pattern with a linewidth of 40 ȝm show numerous pseudopodia which is an indication of cell adhesion. In contrast, rectangular patterns with only one dimension below 10 ȝm do not favour cell adhesion.

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Mammalian Cell Cultures on Micropatterned Surfaces of Weak-Acid, Polyelectrolyte Hyperbranched Thin Films on Gold

Cited by 63 publications

References 70 publications

Cationic polyelectrolyte hydrogel fosters fibroblast spreading, proliferation, and extracellular matrix production: Implications for tissue engineering

Cationic polyelectrolyte hydrogel fosters fibroblast spreading, proliferation, and extracellular matrix production: Implications for tissue engineering

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Laser induced surface structure on stainless steel influences cell viability

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