2013
DOI: 10.1002/elps.201200605
|View full text |Cite
|
Sign up to set email alerts
|

Mammalian cell metabolomics: Experimental design and sample preparation

Abstract: Metabolomics represents the global assessment of metabolites in a biological sample and reports the closest information to the phenotype of the biological system under study. Mammalian cell metabolomics has emerged as a promising tool with potential applications in many biotechnology and research areas. Metabolomics workflow includes experimental design, sampling, sample processing, metabolite analysis, and data processing. Given their influence on metabolite content and biological interpretation of data, a go… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
152
0
1

Year Published

2013
2013
2023
2023

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 176 publications
(162 citation statements)
references
References 81 publications
(167 reference statements)
1
152
0
1
Order By: Relevance
“…Six volumes of PBS (m/v) containing 50 mM NEM were added to each tissue sample. Then, homogenization for 25 s at 6,000×g at 4°C was repeated twice in a Precellys 24 were centrifuged at 10,000×g for 5 min at 4°C and supernatants were transferred to clean tubes. A second consecutive tissue extraction was performed by using four volumes of PBS containing 50 mM NEM.…”
Section: Sample Treatmentsmentioning
confidence: 99%
See 1 more Smart Citation
“…Six volumes of PBS (m/v) containing 50 mM NEM were added to each tissue sample. Then, homogenization for 25 s at 6,000×g at 4°C was repeated twice in a Precellys 24 were centrifuged at 10,000×g for 5 min at 4°C and supernatants were transferred to clean tubes. A second consecutive tissue extraction was performed by using four volumes of PBS containing 50 mM NEM.…”
Section: Sample Treatmentsmentioning
confidence: 99%
“…By 24 h, hepatocytes were shifted to a serum-free medium supplemented with 10 nM insulin and dexamethasone and exposed to 500 μM cumene hydroperoxide for 24 h. After treatments, the culture medium was centrifuged at 5,000×g for 3 min and hepatocyte monolayers were washed twice with PBS. Both culture medium supernatants and cells were immediately frozen in liquid N 2 and stored at −80°C until processed [24].…”
Section: Cultured Rat Hepatocytesmentioning
confidence: 99%
“…Metabolomic analysis aims to define the metabolome, the collection of small biological compounds present in a particular biological sample [6]. While serum or urine samples are typically used, an array of biological samples, including plasma, bronchoalveolar lavage fluid, cerebrospinal fluid, and tissue samples are also suitable for metabolomic analysis [6].…”
Section: Applications Of Metabolomics In Critical Care Medicinementioning
confidence: 99%
“…While serum or urine samples are typically used, an array of biological samples, including plasma, bronchoalveolar lavage fluid, cerebrospinal fluid, and tissue samples are also suitable for metabolomic analysis [6]. Given the relatively small number of metabolites and the relative ease of sample collection, metabolomics presents an efficient and cost-effective alternative to genomics and proteomics analyses.…”
Section: Applications Of Metabolomics In Critical Care Medicinementioning
confidence: 99%
“…Metabolism quenching is always considered as the preliminary step for biological sample preparation, especially before the analysis of cellular metabolome. Many metabolomic studies have optimized the quenching protocols using cold organic solvents, liquid nitrogen and so on [13].…”
Section: Sample Collectionmentioning
confidence: 99%