Mammalian cell response and bacterial adhesion on titanium healing abutments: effect of multiple implantation and sterilization cycles

Jain, Sanjana S.; Siddiqui, Danyal A.; Wheelis, Sutton E.; Palmer, Kelli L.; Wilson, Thomas G.; Rodrigues, Danieli C.

doi:10.1007/s00784-020-03574-0

Cited by 5 publications

(9 citation statements)

References 35 publications

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“…Out of 812 articles, after elimination of articles by title/abstract, 20 articles were selected for full-text reading. Fourteen articles were provisionally selected, of which 8 articles fulfilled the inclusion criteria (Wadhwani et al 2016; Wheelis et al 2018; Chew et al 2018; Stacchi et al 2018; Sánchez-Garcés et al 2019; Jain et al 2020; Almehmadi 2021; Narvekar et al 2020), and 6 were excluded (Vezeau et al 2000; Browne et al 2012; Cakan et al 2015; Kyaw et al 2020; Barreiros et al 2021; Jain et al 2021). The PRISMA flowchart depicting the search strategy and characteristics of included and excluded articles is described in Figure 1 and Table 2, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Reused HAs exhibited signs of mechanical damage even after single use (Jain et al 2020; Barreiros et al 2021; Jain et al 2021). As decontaminated HA abutments have increased surface energy, reusing not only promotes surface degradation but also stimulates deposition of organic products that may be quite tedious to remove (Souza et al 2010; Wadhwani et al 2016; Noronha Oliveira et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Resterilization is “the repeated application of a terminal process designed to remove or destroy all viable forms of microbial life, including bacterial spores, to an acceptable sterility assurance level” (Dunn 2002). Reusing HAs results in surface alterations, biological contamination, and higher corrosion rate, which can contribute to dental implant complications such as perimplantitis (Jain et al 2021). The feasibility of reusing dental components with stringent sterilization protocols, however, has shown promising results in the past decade (Canullo et al 2014; Stacchi et al 2018; Sánchez-Garcés et al 2019; Kyaw et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Contemporary Sterilization Protocols of Healing Abutments for Reusability: A Systematic Review

Eswaramurthy

Aras

D’Souza

et al. 2021

JDR Clinical & Translational Research

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Introduction: Although healing abutments are designated for single use by most implant manufacturers, it is common practice for clinicians to reuse healing abutments. However, there is a lack of adequate references that describe detailed sterilization protocols for reuse of healing abutments. Objectives: The purpose of this systematic review was to compile, organize, and describe the most common techniques for the sterilization of healing abutments and their efficiency in eliminating traces of microorganisms. Methods: An electronic search in 5 different databases was performed, including the National Library of Medicine (MEDLINE via PubMed), Embase, Cochrane Central Register of Controlled Trials, Web of Science, and Google Scholar from January 2000 to December 2020. Search variables included were dental implant, healing abutment, contaminate, contamination, reuse, and sterilization. Studies reporting with a minimum sample size of 10 healing abutments (5 per group) published in the English language were evaluated. Risk of bias assessment was elaborated for included investigations. Results: In total, 812 articles were identified, of which 8 were included in the analysis. Steam autoclave was the most widely used form of resterilization. Not a single protocol, however, was able to achieve 100% virgin surface of the healing abutments. Conclusion: Although reuse of dental implant healing abutments is a cost-effective measure in dental practice, thorough surface decontamination followed by resterilization is highly recommended before reuse. Knowledge Transfer Statement: With consideration of cost and patient preference, results of this review would be useful in knowing various sterilization protocols for reusing healing abutments that could lead to more appropriate therapeutic decisions.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Contemporary Sterilization Protocols of Healing Abutments for Reusability: A Systematic Review

Eswaramurthy

Aras

D’Souza

et al. 2021

JDR Clinical & Translational Research

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“…The healing abutments subjected to repeated chemical, mechanical and physical processes to provide their reuse may undergo surface changes such as oxidation of the titanium coating layer, thus creating porosities ( 6 , 19 ). These porosities are an additional component for the formation of a complex biofilm that provides the adhesion of several bacterial species, including those present in the pathological processes of peri-implant tissues ( 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…In order for the healing abutments to properly exercise their purpose, a decontamination process of this component must be carried out, which consists of the complete removal of organic matter and sterilization, completely eliminating all forms of viral, bacterial and fungal activity ( 2 , 4 , 5 ). Several decontamination methods are used, however, studies point to the presence of residual organic matter and even the presence of viable bacterial activity ( 3 , 4 , 6 , 7 ). It is suggested that the permanence of organic matter and also the maintenance of bacterial viability would entail biological risks, such as contamination of the implant/healing abutment junction and peri-implant tissues which would imply in the impossibility of reusing these components ( 2 , 4 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

Reuse of healing abutments: Ethical, biological and professional training implications

et al. 2022

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Background The aim was to estimate the prevalence of reuse of healing abutments, the methods used in disinfection and to analyze the reasons that lead to the reuse of these components by professionals who work in rehabilitation with dental implants. Material and Methods For this, an online data collection was carried out through a questionnaire developed in Google Forms. This questionnaire was applied to 284 specialists in implantology, randomized, of the 1,147 registered in the Regional Council of Dentistry of Rio Grande do Sul. The questionnaire was divided into three parts: the first containing the Free and Informed Consent Form; the second referring to the correspondents’ demographic data; and the third part with information on reuse, disinfection and sterilization routines used, risk perception and information from manufacturers. To estimate the prevalence in the reuse of healing abutments by implantologists, the frequency of responses was used. Results The results showed that almost all implantologists reuse healing abutments (98.1%). The main reasons for reuse were cost (71.2%) and practicality (26%). Regarding the limitations, 53.3% do not see limitations in its reuse, 20% associate it with increased roughness, 17.8% with the accumulation of organic matter and 8.9% with cross-infection as limitations for reuse. Already 95.3% did not receive any guidance from manufacturers on the reuse of these components. Enzymatic detergent and ultrasonic bath was the most used cleaning method (50.7%) followed by ultrasonic bath (23.3%). Autoclave was the method used for sterilization for all respondents. Conclusions The reuse of healing abutments is a practice adopted by implantologists in the state of Rio Grande do Sul, Brazil and most professionals do not observe limitations in this practice since these components are used repeatedly. Decontamination with enzymatic detergent and an ultrasonic bath is the most commonly used procedure associated with autoclave sterilization. Key words: Abutment, reuse, decontamination, sterilization.

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D-arginine-loaded pH-responsive mesoporous silica nanoparticles enhances the efficacy of water jet therapy in decontaminating biofilm-coated titanium surface

Wang,

Liu,

Chen

et al. 2025

Journal of Controlled Release

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Mammalian cell response and bacterial adhesion on titanium healing abutments: effect of multiple implantation and sterilization cycles

Cited by 5 publications

References 35 publications

Contemporary Sterilization Protocols of Healing Abutments for Reusability: A Systematic Review

Contemporary Sterilization Protocols of Healing Abutments for Reusability: A Systematic Review

Reuse of healing abutments: Ethical, biological and professional training implications

D-arginine-loaded pH-responsive mesoporous silica nanoparticles enhances the efficacy of water jet therapy in decontaminating biofilm-coated titanium surface

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