Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1)

Makarova, Olga; Roh, Michael H.; Liu, Chia Jen; Laurinec, Stephanie; Margolis, Ben

doi:10.1016/s0378111902010843

Cited by 177 publications

(217 citation statements)

References 27 publications

Supporting

Mentioning

205

Contrasting

Order By: Relevance

“…We never observed delivery of Crumbs3a to the periciliary region. Crumbs3a forms stable attachments with a submembranous anchoring complex that includes the proteins PALS and PATJ (Makarova et al, 2003), and transmembrane gp135 has been shown to interact directly with cytoskeletal components (Ojakian and Schwimmer, 1988;Li et al, 2002;Nielsen et al, 2007;Yu et al, 2007;Francis et al, 2011). Thus, we next wished to examine the behavior of a membrane protein that cannot participate in such interactions.…”

Section: Resultsmentioning

confidence: 99%

The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135

Stoops¹,

Hull²,

Olesen³

et al. 2015

J Gen Physiol

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135

Stoops¹,

Hull²,

Olesen³

et al. 2015

J Gen Physiol

View full text Add to dashboard Cite

“…3D). This is surprising because PATJ associates with tight junctions in MDCK cells (Shin et al, 2005), where it interacts with PALS1 (Roh et al, 2002;Straight et al, 2006) and Crumbs (Makarova et al, 2003).…”

Section: Mals-3 Is Associated With Cell Membranesmentioning

confidence: 99%

MALS-3 regulates polarity and early neurogenesis in the developing cerebral cortex

et al. 2008

View full text Add to dashboard Cite

Apicobasal polarity plays an important role in regulating asymmetric cell divisions by neural progenitor cells (NPCs) in invertebrates, but the role of polarity in mammalian NPCs is poorly understood. Here, we characterize the function of the PDZ domain protein MALS-3 in the developing cerebral cortex. We find that MALS-3 is localized to the apical domain of NPCs. Mice lacking all three MALS genes fail to localize the polarity proteins PATJ and PALS1 apically in NPCs, whereas the formation and maintenance of adherens junctions appears normal. In the absence of MALS proteins, early NPCs progressed more slowly through the cell cycle, and their daughter cells were more likely to exit the cell cycle and differentiate into neurons. Interestingly, these effects were transient; NPCs recovered normal cell cycle properties during late neurogenesis. Experiments in which MALS-3 was targeted to the entire membrane resulted in a breakdown of apicobasal polarity, loss of adherens junctions, and a slowing of the cell cycle. Our results suggest that MALS-3 plays a role in maintaining apicobasal polarity and is required for normal neurogenesis in the developing cortex.

show abstract

“…The Crumbs proteins are good candidates for the mediation for the following reasons: First, Crumbs, a transmembrane protein initially identified in fly that interacts with Stardust, the fly homolog of Nok, has three homologs in vertebrates: Crb1, Crb2, and Crb3 (Tepass and Knust, 1993;Hong, et al, 2001;Makarova et al, 2003;Katoh, 2004;den Hollander, 2001b). Second, Crb1, 2, and 3 have been observed to be expressed in the ONL in the mouse retina and Crb1 physically interacts with Pals1, the mouse homolog of Nok, in retinal lysates (van de Pavert et al, 2004).…”

Section: Evidence For the Possibility Of A Nok-mediated Photoreceptormentioning

confidence: 99%

Nok plays an essential role in maintaining the integrity of the outer nuclear layer in the zebrafish retina

Wei

Zou

Takechi

et al. 2006

Experimental Eye Research

View full text Add to dashboard Cite

Proper visual function of the vertebrate retina requires the maintenance of the integrity of the retinal outer nuclear layer (ONL), which is often affected in many blinding human retinal diseases.While the structural integrity of the ONL has long been considered to be maintained primarily through the outer limiting membrane (OLM), we have little knowledge on the development and maintenance of the OLM itself. Here, by analyzing the adhering properties of photoreceptors in zebrafish N-cad and nok mutants, we demonstrated for the first time that the nok gene is essential for the establishment and/or maintenance of the OLM. In addition, our results imply the possibility that Nok, Crumbs, and their associated proteins may constitute a type of photoreceptor-photoreceptor junctional complex that has not been described before. Thus, our study provides novel insights into the mechanisms by which the integrity of the ONL is maintained in the vertebrate retina.

show abstract

Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1)

Cited by 177 publications

References 27 publications

The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135

The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135

MALS-3 regulates polarity and early neurogenesis in the developing cerebral cortex

Nok plays an essential role in maintaining the integrity of the outer nuclear layer in the zebrafish retina

Contact Info

Product

Resources

About