Mammalian Fuel Cells Produce Electric Current

Shlosberg, Yaniv; Faynus, Mohamed A.; Huang, Ailun; Carlini, Andrea S.; Clegg, Dennis O.; Kaner, Richard B.

doi:10.1021/acsami.3c06019

Cited by 7 publications

(8 citation statements)

References 51 publications

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“…1b ). Voltammograms show a peak with a maximum at 0.9 V, which we correlate with the fingerprint of NADH 41 At this potential, NADH can be oxidized at the anode. To assess this, the fluorescence spectra (λ Ex = 300 nm, λ Em = 450 nm) of LB after 1, 3 and 5 cycles of CV was measured ( Fig.…”

Section: Resultssupporting

confidence: 61%

“…This can be addressed by using photosynthetic microorganisms [33][34][35] or live macro-organisms (e.g. seaweeds, plant leaves/roots, mammalian fuel cells, and sea-anemones [36][37][38][39][40][41][42] ) that act as electron donors in bioelectrochemical cells (BECs). Live organisms can act as a continuous source of reducing molecule generation for long term electricity production.…”

Section: Introductionmentioning

confidence: 99%

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Redox-active molecules in bacterial cultivation media produce photocurrent

Shlosberg

Smith

Carlini

2022

Preprint

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In recent years, global climate change has urged the importance of developing new clean energy technologies that may replace the combustion of fossil fuels and help to reduce carbon emissions. A promising method is the utilization of bacteria as electron donors in microbial fuel cells. In this work, we show that the cultivation media of bacteria that is based on yeast extract consists of redox active molecules that can be used as electron donors reducing the electron acceptor Cytochrome C and producing an electrical current in a bio-electrochemical cell. We apply cyclic voltammetry and 2-dimensional fluorescence spectra to identify the redox active molecules NADH and flavines that may play a key role in the electron donation mechanism. Finally, we show that upon illumination, the current production is enhanced.

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Section: Resultssupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Redox-active molecules in bacterial cultivation media produce photocurrent

Shlosberg

Smith

Carlini

2022

Preprint

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“…A small current increase of about 1 µA / cm 2 was obtained around 400s which turned into a relatively constant current from 700 to 1500 s. We suggest that this current represent an equilibrium in which the rate of the electron donors release is almost equal to the rate of their consumption. The maximal current density between 400 to 1500 s was about 7.5 µA / cm 2 which is similar to the current density previously obtained by ARPE19 cells 39 .…”

Section: Live Fibroblast Cells Produce Electrical Current In a Becsupporting

confidence: 89%

“…A previous work has reported for the first time the use of mammalian cells as electron donors when integrated in a BEC 39 using ARPE19 eyes cell line. The current production by these cells was partially generated by the release of NADH and flavins to the external cell solution (ECS) which can act as electron donors.…”

Section: Resultsmentioning

confidence: 99%

Bio-electricity production from Fibroblasts and their cultivation media

Shlosberg,

Lesman

2024

Preprint

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The recent understanding of the critical future damage that might happen on earth by climate change has urged scientists to initiate new creative ideas for clean energy technologies that will reduce carbon emissions. A promising approach is the utilization of living cells as electron donors in bio-electrochemical cells (BECs). This concept has been intensively studied for micro-organisms such as non-photosynthetic bacteria, cyanobacteria, and microalgae, but not for mammalian cells. In this work, we report for the first-time integrating live fibroblast cells in a BEC to produce electrical current that is about 3 times higher than intact micro-organisms. Furthermore, we apply 2D-fluorescence and electrochemical measurements to show that like in micro-organisms based BECs, NADH and flavins play a role in the electron mediation between the cells and the anode. Finally, we show that the cultivation medium of fibroblasts also consists of redox species that may produce dark and photocurrent.

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“…The refinement of MFC technology for bacterial cell culture creates opportunities for applications in multicellular organisms, such as mammalian fuel cells that produce electrical current from electron donors produced by cellular metabolism . At the subcellular scale, the immobilization of proteins such as glucose oxidase on an electrode’s surface allows for the generation of electricity from glucose, a readily available molecule in multicellular organisms . Metabolic fuel cells have been used to power a variety of implanted devices, such as an implanted brain stimulator in a bird, offering a significant weight advantage compared to an onboard battery .…”

Section: Electrical Interfacesmentioning

confidence: 99%

Information Transmission through Biotic–Abiotic Interfaces to Restore or Enhance Human Function

Kelly,

Glover

2024

ACS Appl. Bio Mater.

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Advancements in reliable information transfer across biotic− abiotic interfaces have enabled the restoration of lost human function. For example, communication between neuronal cells and electrical devices restores the ability to walk to a tetraplegic patient and vision to patients blinded by retinal disease. These impactful medical achievements are aided by tailored biotic− abiotic interfaces that maximize information transfer fidelity by considering the physical properties of the underlying biological and synthetic components. This Review develops a modular framework to define and describe the engineering of biotic and abiotic components as well as the design of interfaces to facilitate biotic−abiotic information transfer using light or electricity. Delineating the properties of the biotic, interface, and abiotic components that enable communication can serve as a guide for future research in this highly interdisciplinary field. Application of synthetic biology to engineer light-sensitive proteins has facilitated the control of neural signaling and the restoration of rudimentary vision after retinal blindness. Electrophysiological methodologies that use brain− computer interfaces and stimulating implants to bypass spinal column injuries have led to the rehabilitation of limb movement and walking ability. Cellular interfacing methodologies and on-chip learning capability have been made possible by organic transistors that mimic the information processing capacity of neurons. The collaboration of molecular biologists, material scientists, and electrical engineers in the emerging field of biotic−abiotic interfacing will lead to the development of prosthetics capable of responding to thought and experiencing touch sensation via direct integration into the human nervous system. Further interdisciplinary research will improve electrical and optical interfacing technologies for the restoration of vision, offering greater visual acuity and potentially color vision in the near future.

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Mammalian Fuel Cells Produce Electric Current

Cited by 7 publications

References 51 publications

Redox-active molecules in bacterial cultivation media produce photocurrent

Redox-active molecules in bacterial cultivation media produce photocurrent

Bio-electricity production from Fibroblasts and their cultivation media

Information Transmission through Biotic–Abiotic Interfaces to Restore or Enhance Human Function

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