Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species

Rossello, Ricardo; Chen, Chun‐Chun; Dai, Rui; Howard, Jason T.; Hochgeschwender, Ute; Jarvis, Erich D.

doi:10.7554/elife.00036

Cited by 79 publications

(76 citation statements)

References 87 publications

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“…iPSCs were first derived from mouse fibroblasts by overexpression of four reprogramming factors-Oct4, Sox2, Klf4, and c-Myc-and subsequently from human fibroblasts by overexpression of OCT4, SOX2, LIN28, and NANOG (Takahashi and Yamanaka, 2006;Yu et al, 2007) after retroviral transfection. Retroviral and lentiviral delivery of core reprogramming factors allowed the derivation of iPSCs from several other mammals, including rat (Liao et al, 2009), dog (Shimada et al, 2010), pig (Esteban et al, 2009), rhesus monkey (Liu et al, 2008), sheep (Bao et al, 2011), goat , horse (Nagy et al, 2011), cattle (Han et al, 2011), buffalo (Deng et al, 2012), and of iPS-like cells from nonmammalian vertebrates (Rossello et al, 2013). iPSCs derived from somatic tissue are particularly promising in farm animals in which true, germline-competent embryonic stem cells (ESCs) could not yet be derived (Kumar et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Talluri

Kumar

Glage

et al. 2015

Cellular Reprogramming

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Induced pluripotent stem cells (iPSCs) are a seminal breakthrough in stem cell research and are promising tools for advanced regenerative therapies in humans and reproductive biotechnology in farm animals. iPSCs are particularly valuable in species in which authentic embryonic stem cell (ESC) lines are yet not available. Here, we describe a nonviral method for the derivation of bovine iPSCs employing Sleeping Beauty (SB) and piggyBac (PB) transposon systems encoding different combinations of reprogramming factors, each separated by self-cleaving peptide sequences and driven by the chimeric CAGGS promoter. One bovine iPSC line (biPS-1) generated by a PB vector containing six reprogramming genes was analyzed in detail, including morphology, alkaline phosphatase expression, and typical hallmarks of pluripotency, such as expression of pluripotency markers and formation of mature teratomas in immunodeficient mice. Moreover, the biPS-1 line allowed a second round of SB transposon-mediated gene transfer. These results are promising for derivation of germ linecompetent bovine iPSCs and will facilitate genetic modification of the bovine genome.

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Section: Introductionmentioning

confidence: 99%

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Talluri

Kumar

Glage

et al. 2015

Cellular Reprogramming

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“…We showed that Xenopus differentiated skeletal muscle fibers could be reprogrammed to pluripotency after transfecting the Yamanaka factors (mouse Oct4, Sox2 and Klf4). This was the first time that induced reprogramming was reported to be feasible in vivo and that mammalian factors were shown to be able to reprogram nonmammalian cells (Vivien et al 2012); others have confirmed these observations (Abad et al 2013;Rosselló et al 2013). This safe and inexpensive technique has been adapted to fish (Hansen et al 1991) including zebrafish (L. Coen, unpublished data), Xenopus tropicalis (Rowe et al 2002), and used in various species to develop immunization protocols (Donnelly et al 1995;Lewis and Babiuk 1999;Pachuk et al 2000).…”

Section: Discussionmentioning

confidence: 72%

In Vivo Transfection of Naked DNA into Xenopus Tadpole Tail Muscle

Marshall

Girardot

Demeneix

et al. 2017

Cold Spring Harb Protoc

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In vivo gene transfer systems are important to study foreign gene expression and promoter regulation in an organism, with the benefit of exploring this in an integrated environment. Direct injection of plasmids encoding exogenous promoters and genes into muscle has numerous advantages: the protocol is easy, efficient, and shows time-persistent plasmid expression in transfected muscular cells. After injecting naked-DNA plasmids into tadpole tail muscle, transgene expression is strong, reproducible, and correlates with the amount of DNA injected. Moreover, expression is stable as long as the tadpoles remain, or are maintained, in premetamorphic stages. By directly expressing genes and regulated promoters in Xenopus tadpole muscle in vivo, one can exploit the powerful experimental advantages of gene transfer systems in an intact, physiologically normal animal.

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“…A recent report has also demonstrated the development of ciPSCs capable of producing chimeric chickens (Rossello et al, 2013). These cells were developed using viral approaches, yet they still demonstrated limited incorporation in embryonic chicks, even when using low-passage cells.…”

Section: Discussionmentioning

confidence: 96%

Nonviral Minicircle Generation of Induced Pluripotent Stem Cells Compatible with Production of Chimeric Chickens

Jordan

et al. 2014

Cellular Reprogramming

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Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such as meat and egg production or even disease resistance. However, these cell types have significant limitations because they are hard to culture long term while maintaining developmental plasticity. Induced pluripotent stem cells (iPSCs) are a novel class of stem cells that have proven to be robust, leading to the successful development of transgenic mice, rats, quail, and pigs and may potentially overcome the limitations of previous pluripotent stem cell systems in chickens. In this study we generated chicken (c) iPSCs from fibroblast cells for the first time using a nonviral minicircle reprogramming approach. ciPSCs demonstrated stem cell morphology and expressed key stem cell markers, including alkaline phosphatase, POU5F1, SOX2, NANOG, and SSEA-1. These cells were capable of rapid growth and expressed high levels of telomerase. Late-passage ciPSCs transplanted into stage X embryos were successfully incorporated into tissues of all three germ layers, and the gonads demonstrated significant cellular plasticity. These cells provide an exciting new tool to create transgenic chickens with broad implications for agricultural and transgenic animal fields at large.

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Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species

Cited by 79 publications

References 87 publications

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming

In Vivo Transfection of Naked DNA into Xenopus Tadpole Tail Muscle

Nonviral Minicircle Generation of Induced Pluripotent Stem Cells Compatible with Production of Chimeric Chickens

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