Mammalian Mediator 19 Mediates H1299 Lung Adenocarcinoma Cell Clone Conformation, Growth, and Metastasis

Xu, Lulu; ShuLiang, Guo; Ma, Su-Ren; Luo, Yi

doi:10.7314/apjcp.2012.13.8.3695

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…In line with our and our colleagues' previous discoveries, LCMR1 is positively associated with the proliferative ability of human NSCLC cells [10][11][12][13]. Here, we showed that…”

Section: Discussionsupporting

confidence: 93%

“…In line with our and our colleagues' previous discoveries, LCMR1 is positively associated with the proliferative ability of human NSCLC cells [10][11][12][13]. Here, we showed that LCMR1 can suppress the in vitro and in vivo proliferation of 95D cells-a subcloned cell line derived from a poorly differentiated human LCLC.…”

Section: Discussionsupporting

confidence: 91%

“…Although LCMR1 was first cloned in LCLC cells, many reports have since revealed that it regulates the proliferation and migration of breast cancer [6], prostate cancer [7], gastric cancer [8], and tongue cancer [9] cells. In lung adenocarcinoma cells, knocking down LCMR1 reduces cell proliferation and tumorigenesis [10][11][12]; yet its role in LCLC, where it was originally identified, remains to be established [13,14].…”

Section: Introductionmentioning

confidence: 99%

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LCMR1 Promotes Large-Cell Lung Cancer Proliferation and Metastasis by Downregulating HLA-Encoding Genes

Liu,

Li,

et al. 2023

Cancers

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Lung cancer is notorious for its high global morbidity and mortality. Here, we examined whether the LCMR1 gene, which we previously cloned from a human large-cell lung carcinoma cell line, contributes to the proliferation and metastasis of large-cell lung carcinoma. To this end, we performed pan-cancer and non-small cell lung cancer (NSCLC) cell line-based LCMR1 expression profiling. Results revealed that LCMR1 was expressed at high levels in most solid tumors, including NSCLC. LCMR1 expression was the highest in the 95D large cell lung cancer cell line. Functional studies using lentivirus-based knockdown revealed that LCMR1 was critical for the proliferation, migration, and invasion of cultured large cell lung cancer cells. Moreover, blocking this gene significantly reduced tumor growth in a 95D cell xenograft mouse model. A multiple sequence-based assay revealed a mechanism by which LCMR1 diminished the RNA Pol II occupancy at the promoter of human leukocyte antigen (HLA)-encoding genes to prevent their transcription. The HLA genes play vital roles in cancer-specific antigen presentation and anticancer immunity. A correlation assay using TCGA database identified a negative relationship between the expression levels of LCMR1 and HLA coding genes. Taken together, our findings demonstrate that LCMR1 is required for large cell lung cancer cell growth and invasion and suggest its potential as a valid target in clinical treatment.

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“…In line with our and our colleagues' previous discoveries, LCMR1 is positively associated with the proliferative ability of human NSCLC cells [10][11][12][13]. Here, we showed that…”

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LCMR1 Promotes Large-Cell Lung Cancer Proliferation and Metastasis by Downregulating HLA-Encoding Genes

Liu,

Li,

et al. 2023

Cancers

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“…In breast cancer, Med19 may promote cell proliferation by regulating CBFA2T3/HEB expression [ 13 ]. In lung cancer, MED19 could promote tumor proliferation, tumorigenesis, metastasis, and enhance chemo-sensitivity to cisplatin in non-small cell lung cancer cells [ 14 – 16 ]. The Med19 has been reported to promote tumor growth and/or metastasis in ovarian cancer, gastric cancer, pancreatic cancer, osteosarcoma, and liver cancer [ 5 – 7 , 17 – 18 ].…”

Section: Discussionmentioning

confidence: 99%

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells

Wang

Yuan

et al. 2017

PLoS ONE

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Prostate cancer (PCa) is one of the most common cancers in elderly men. Mediator Complex Subunit 19 (Med19) is overexpressed and plays promotional roles in many cancers. However, the roles of Med19 in PCa are still obscure. In this study, by using immunohistochemical staining, we found higher expression level of Med19 in PCa tissues than in adjacent benign prostate tissues. We then knocked down the Med19 expression in PCa cell lines LNCaP and PC3 by using lentivirus siRNA. Cell proliferation, anchor-independent growth, migration, and invasion were suppressed in Med19 knockdown PCa cells. In nude mice xenograft model, we found that Med19 knockdown PCa cells formed smaller tumors with lower proliferation index than did control cells. In the mechanism study, we found that Med19 could regulate genes involved in cell proliferation, cell cycle, and epithelial-mesenchymal transition, including P27, pAKT, pPI3K, IGF1R, E-Cadherin, N-Cadherin, Vimentin, ZEB2, Snail-1 and Snail-2. Targeting Med19 in PCa cells could inhibit the PCa growth and metastasis, and might be a therapeutic option for PCa in the future.

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Involvement of Mediator complex in malignancy

Schiano

Casamassimi

Rienzo

et al. 2014

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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Mammalian Mediator 19 Mediates H1299 Lung Adenocarcinoma Cell Clone Conformation, Growth, and Metastasis

Cited by 5 publications

References 21 publications

LCMR1 Promotes Large-Cell Lung Cancer Proliferation and Metastasis by Downregulating HLA-Encoding Genes

LCMR1 Promotes Large-Cell Lung Cancer Proliferation and Metastasis by Downregulating HLA-Encoding Genes

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells

Involvement of Mediator complex in malignancy

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