Mammalian Sperm Contain a Ca2+-Sensitive Phospholipase C Activity That Can Generate InsP3 from PIP2 Associated with Intracellular Organelles

Rice, Anne; Parrington, John; Jones, Kevin; Swann, Karl

doi:10.1006/dbio.2001.0477

Cited by 38 publications

(58 citation statements)

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“…The contention that this sperm PLC activity is significant is supported by the finding that it is two orders of magnitude greater than that found in other mammalian tissue extracts prepared in the same way as spermatozoa (Rice et al, 2000). The PLC activity in a single spermatozoon is such that a rough estimate indicates it may be sufficient to account for Ca 2+ release in a mammalian egg (Rice et al, 2000).…”

Section: How Does the Sperm Factor Cause Ca 2+ Release?supporting

confidence: 65%

Potential role of a sperm-derived phospholipase C in triggering the egg-activating Ca2+ signal at fertilization

Swann

Parrington²,

Jones³

2001

Reproduction

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An increase in intracellular Ca2+ at fertilization is the trigger for egg activation in all species that have been studied. Exactly how sperm-egg interaction leads to this Ca2+ increase has not been established. There is increasing support for the hypothesis that the spermatozoon introduces a Ca2+-releasing protein into the egg cytoplasm after gamete membrane fusion. This review discusses the merits of this 'sperm factor' hypothesis and presents evidence indicating that the sperm factor, at least in mammals, consists of a phospholipase C with distinctive properties. This evidence leads us to propose that, after gamete fusion, a sperm-derived phospholipase C causes production of inositol 1,4,5- trisphosphate, which then generates Ca2+ waves from within the egg cytoplasm.

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Section: How Does the Sperm Factor Cause Ca 2+ Release?supporting

confidence: 65%

Potential role of a sperm-derived phospholipase C in triggering the egg-activating Ca2+ signal at fertilization

Swann

Parrington²,

Jones³

2001

Reproduction

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“…In this regard, in vitro PLC assays demonstrated that boar sperm extracts have PLC activity, and that this activity is stimulated by increases in Ca 2+ concentration (Jones et al, 1998aRice et al, 2000). In considering the role of a sperm PLC(s) as SF, it is worth noting that in terms of volume and protein content, the egg is exceedingly larger than the sperm, limiting the plausibility that SF is a PLC isozyme already present in eggs.…”

Section: Sperm Plcmentioning

confidence: 99%

Mammalian Fertilization: From Sperm Factor to Phospholipase Cζ

Kurokawa

Sato

Fissore

2004

Biology of the Cell

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In mammalian eggs, the fertilizing sperm evokes intracellular Ca 2+ ([Ca 2+ ] i ) oscillations that are essential for initiation of egg activation and embryonic development. Although the exact mechanism leading to initiation of [Ca 2+ ] i oscillations still remains unclear, accumulating studies suggest that a presently unknown substance, termed sperm factor (SF), is delivered from the fertilizing sperm into the ooplasm and triggers [Ca 2+ ] i oscillations. Based on findings showing that production of inositol 1,4,5-trisphosphate (IP 3 ) underlies the generation of [Ca 2+ ] i oscillations, it has been suggested that SF functions either as a phospholipase C (PLC), an enzyme that catalyzes the generation of IP 3 , or as an activator of a PLC(s) pre-existing in the egg. This review discusses the role of SF as the molecule responsible for the production of IP 3 and the initiator of [Ca 2+ ] i oscillations in mammalian fertilization, with particular emphasis on the possible involvement of egg-and sperm-derived PLCs, including PLCf, a novel sperm specific PLC.

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“…1). The recent finding that the mammalian sperm factor is possibly a Ca 2+ -activated phospholipase C (PLC) (Rice et al, 2000;Saunders et al, 2002) argues in favor of Ins(1,4,5)P3 oscillations driving sperm-triggered Ca 2+ oscillations in eggs. Indeed, the prolonged stimulation of a Ca 2+ -activated PLC can result in Ca 2+ oscillations regulated by an oscillating production of Ins(1,4,5)P3 (for details, see Meyer and Stryer, 1988).…”

Section: The Organization Of the Er Network May Regulate The Ca 2+ Wamentioning

confidence: 99%

Calcium wave pacemakers in eggs

Dumollard

Carroll

Dupont

et al. 2002

Journal of Cell Science

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During the past 25 years, the characterization of sperm-triggered calcium signals in eggs has progressed from the discovery of a single calcium increase at fertilization in the medaka fish to the observation of repetitive calcium waves initiated by multiple meiotic calcium wave pacemakers in the ascidian. In eggs of all animal species, sperm-triggered inositol (1,4,5)-trisphosphate[Ins(1,4,5)P3] production regulates the vast array of calcium wave patterns observed in the different species. The spatial organization of calcium waves is driven either by the intracellular distribution of the calcium release machinery or by the localized and dynamic production of calcium-releasing second messengers. In the highly polarized egg cell, cortical endoplasmic reticulum (ER)-rich clusters act as pacemaker sites dedicated to the initiation of global calcium waves. The extensive ER network made of interconnected ER-rich domains supports calcium wave propagation throughout the egg. Fertilization triggers two types of calcium wave pacemakers depending on the species: in mice, the pacemaker site in the vegetal cortex of the egg is probably a site that has enhanced sensitivity to Ins(1,4,5)P3; in ascidians, the calcium wave pacemaker may rely on a local source of Ins(1,4,5)P3 production apposed to a cluster of ER in the vegetal cortex.

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Mammalian Sperm Contain a Ca2+-Sensitive Phospholipase C Activity That Can Generate InsP3 from PIP2 Associated with Intracellular Organelles

Cited by 38 publications

References 0 publications

Potential role of a sperm-derived phospholipase C in triggering the egg-activating Ca2+ signal at fertilization

Potential role of a sperm-derived phospholipase C in triggering the egg-activating Ca2+ signal at fertilization

Mammalian Fertilization: From Sperm Factor to Phospholipase Cζ

Calcium wave pacemakers in eggs

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